Ginseng-Derived Panaxadiol Saponins Promote Hematopoiesis Recovery in Cyclophosphamide-Induced Myelosuppressive Mice: Potential Novel Treatment of Chemotherapy-Induced Cytopenias

Xin Sun; Yan-na Zhao; Song Qian; Rui-lan Gao; Li-ming Yin; Li-pei Wang; Beng-hock Chong; Su-zhan Zhang

doi:10.1007/s11655-017-2754-8

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Ginseng-Derived Panaxadiol Saponins Promote Hematopoiesis Recovery in Cyclophosphamide-Induced Myelosuppressive Mice: Potential Novel Treatment of Chemotherapy-Induced Cytopenias

OriginalPaper | Updated：2021-08-27

- Ginseng-Derived Panaxadiol Saponins Promote Hematopoiesis Recovery in Cyclophosphamide-Induced Myelosuppressive Mice: Potential Novel Treatment of Chemotherapy-Induced Cytopenias
- Ginseng-Derived Panaxadiol Saponins Promote Hematopoiesis Recovery in Cyclophosphamide-Induced Myelosuppressive Mice: Potential Novel Treatment of Chemotherapy-Induced Cytopenias
- 中国结合医学杂志（英文版） 2018年24卷第3期页码：200-206
- Affiliations：
  
  1. Department of Oncology, The Second Affiliated Hospital, Medical College of Zhejiang University,Hangzhou,China
  2. Department of Oncology, Zhejiang Provincial People’s Hospital,Hangzhou,China
  3. Institution of Hematology Research, The First Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou,China
  4. Department of Hematology, St George Sutherland Hospital,Sydney,Australia
- Author bio：
- Funds：
  
  Supported by the Science and Technology Program Project of Zhejiang Province (No. 2015C33173), and Chinese Medicine Foundation of Young Talents in Zhejiang Province (No. 2014ZQ006), Australia-China Institutional Links Research Program sponsored by the International Development Program of Education Australia (No. IDP 2-8)
- DOI：10.1007/s11655-017-2754-8
  中图分类号：
- 纸质出版日期：2018，
  
  网络出版日期：2017-4-22，
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Sun, X., Zhao, Yn., Qian, S. et al. Ginseng-Derived Panaxadiol Saponins Promote Hematopoiesis Recovery in Cyclophosphamide-Induced Myelosuppressive Mice: Potential Novel Treatment of Chemotherapy-Induced Cytopenias., Chin. J. Integr. Med. 24, 200–206 (2018). https://doi.org/10.1007/s11655-017-2754-8

Xin Sun, Yan-na Zhao, Song Qian, et al. Ginseng-Derived Panaxadiol Saponins Promote Hematopoiesis Recovery in Cyclophosphamide-Induced Myelosuppressive Mice: Potential Novel Treatment of Chemotherapy-Induced Cytopenias[J]. Chinese Journal of Integrative Medicine, 2018,24(3):200-206.
Sun, X., Zhao, Yn., Qian, S. et al. Ginseng-Derived Panaxadiol Saponins Promote Hematopoiesis Recovery in Cyclophosphamide-Induced Myelosuppressive Mice: Potential Novel Treatment of Chemotherapy-Induced Cytopenias., Chin. J. Integr. Med. 24, 200–206 (2018). https://doi.org/10.1007/s11655-017-2754-8 DOI：

Xin Sun, Yan-na Zhao, Song Qian, et al. Ginseng-Derived Panaxadiol Saponins Promote Hematopoiesis Recovery in Cyclophosphamide-Induced Myelosuppressive Mice: Potential Novel Treatment of Chemotherapy-Induced Cytopenias[J]. Chinese Journal of Integrative Medicine, 2018,24(3):200-206. DOI： 10.1007/s11655-017-2754-8.

摘要

To investigate the potential efficacy of panaxadiol saponins component (PDS-C)

a biologically active fraction isolated from total ginsenosides

to reverse chemotherapy-induced myelosuppression and pancytopenia caused by cyclophamide (CTX). Mice with myelosuppression induced by CTX were treated with PDS-C at a low- (20 mg/kg)

moderate- (40 mg/kg)

or high-dose (80 mg/kg) for 7 consecutive days. The level of peripheral white blood cell (WBC)

neutrophil (NEU) and platelet (PLT) were measured

the histopathology and colony formation were observed

the protein kinase and transcription factors in hematopoietic cells were determined by immunohistochemical staining and Western blot. In response to PDS-C therapy

the peripheral WBC

NEU and PLT counts of CTX-induced myelosuppressed mice were significantly increased in a dose-dependent manner. Similarly

bone marrow histopathology examination showed reversal of CTX-induced myelosuppression with increase in overall bone marrow cellularity and the number of hematopoietic cells (P<0.01). PDS-C also promoted proliferation of granulocytic and megakaryocyte progenitor cells in CTX-treated mice

as evidenced by significantly increase in colony formation units-granulocytes/monocytes and -megakaryocytes (P<0.01). The enhancement of hematopoiesis by PDS-C appears to be mediated by an intracellular signaling pathway

this was evidenced by the up-regulation of phosphorylated mitogen-activated protein kinase (p-MEK) and extracellular signal-regulated kinases (p-ERK)

and receptor tyrosine kinase (C-kit) and globin transcription factor 1 (GATA-1) in hematopoietic cells of CTX-treated mice (P<0.05). PDS-C possesses hematopoietic growth factor-like activities that promote proliferation and also possibly differentiation of hematopoietic progenitor cells in myelosuppressed mice

probably mediated by a mechanism involving MEK and ERK protein kinases

and C-kit and GATA-1 transcription factors. PDS-C may potentially be a novel treatment of myelosuppression and pancytopenia caused by chemotherapy.

Abstract

To investigate the potential efficacy of panaxadiol saponins component (PDS-C)

a biologically active fraction isolated from total ginsenosides

to reverse chemotherapy-induced myelosuppression and pancytopenia caused by cyclophamide (CTX). Mice with myelosuppression induced by CTX were treated with PDS-C at a low- (20 mg/kg)

moderate- (40 mg/kg)

or high-dose (80 mg/kg) for 7 consecutive days. The level of peripheral white blood cell (WBC)

neutrophil (NEU) and platelet (PLT) were measured

the histopathology and colony formation were observed

the protein kinase and transcription factors in hematopoietic cells were determined by immunohistochemical staining and Western blot. In response to PDS-C therapy

the peripheral WBC

NEU and PLT counts of CTX-induced myelosuppressed mice were significantly increased in a dose-dependent manner. Similarly

this was evidenced by the up-regulation of phosphorylated mitogen-activated protein kinase (p-MEK) and extracellular signal-regulated kinases (p-ERK)

probably mediated by a mechanism involving MEK and ERK protein kinases

and C-kit and GATA-1 transcription factors. PDS-C may potentially be a novel treatment of myelosuppression and pancytopenia caused by chemotherapy.

关键词

panaxadiol saponins componentGinsenosideschemotherapy-induced myelosuppressionmitogen-activated protein kinaseextracellular signal-regulated kinasereceptor tyrosine kinaseglobin transcription factor 1Chinese Medicine

Keywords

references

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