Therapeutic Effect of Ershen Pill (二神丸) Extract on Pi (Spleen)-Shen (Kidney) Yang Deficiency-Induced Diarrhea in Rat Model

Xin PAN; Kang XU; Rui XIONG; Zhi-min CHEN; Chang-jiang HU; Jian FENG; Wen-bin LI

doi:10.1007/s11655-017-2766-4

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Therapeutic Effect of Ershen Pill (二神丸) Extract on Pi (Spleen)-Shen (Kidney) Yang Deficiency-Induced Diarrhea in Rat Model

Original Article | Updated：2021-08-27

- Therapeutic Effect of Ershen Pill (二神丸) Extract on Pi (Spleen)-Shen (Kidney) Yang Deficiency-Induced Diarrhea in Rat Model
- Therapeutic Effect of Ershen Pill (二神丸) Extract on Pi (Spleen)-Shen (Kidney) Yang Deficiency-Induced Diarrhea in Rat Model
- Chinese Journal of Integrative Medicine 2019年25卷第7期页码：536-542
- Affiliations：
  
  1.College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu (610075), China
  2.College of Pharmacy, South Central University for Nationalities, Wuhan (430074), China
  3.National Innovation and Attracting Talents "111" Base, Bioengineering College, Chongqing University, Chongqing (400030), China
  4.University of California, Berkeley-Chongqing University Joint PhD. Student Program in Bioengineering, Stanley Hall, Berkeley, CA (94720), United States
  5.Key Research Institute of Quality and Efficacy Evaluation of Traditional Chinese Medicine Formula, State Administration of Traditional Chinese Medicine, Chengdu (610075), China
- Author bio：
  
  Correspondence to: Dr. LI Wen-bin, Tel: 86-28-83705888, E-mail: 285892232@qq.com
- Funds：
  
  the National Natural Science Foundation of China(81274085)
- DOI：10.1007/s11655-017-2766-4
  中图分类号：
- 纸质出版日期：2019-07-01，
  
  网络出版日期：2018-03-21，
  
  录用日期：2016-04-12
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Xin PAN, Kang XU, Rui XIONG, 等. Therapeutic Effect of Ershen Pill (二神丸) Extract on Pi (Spleen)-Shen (Kidney) Yang Deficiency-Induced Diarrhea in Rat Model[J]. Chinese Journal of Integrative Medicine, 2019,25(7):536-542.

Xin PAN, Kang XU, Rui XIONG, et al. Therapeutic Effect of Ershen Pill (二神丸) Extract on Pi (Spleen)-Shen (Kidney) Yang Deficiency-Induced Diarrhea in Rat Model[J]. Chinese Journal of Integrative Medicine, 2019,25(7):536-542.
Xin PAN, Kang XU, Rui XIONG, 等. Therapeutic Effect of Ershen Pill (二神丸) Extract on Pi (Spleen)-Shen (Kidney) Yang Deficiency-Induced Diarrhea in Rat Model[J]. Chinese Journal of Integrative Medicine, 2019,25(7):536-542. DOI： 10.1007/s11655-017-2766-4.

Xin PAN, Kang XU, Rui XIONG, et al. Therapeutic Effect of Ershen Pill (二神丸) Extract on Pi (Spleen)-Shen (Kidney) Yang Deficiency-Induced Diarrhea in Rat Model[J]. Chinese Journal of Integrative Medicine, 2019,25(7):536-542. DOI： 10.1007/s11655-017-2766-4.

摘要

Abstract

Objective:

To investigate whether Ershen Pill (ESP

二神丸) could alleviate the symptom of Pi (Spleen)-Shen (Kidney) yang deficiency (PSYD)-induced diarrhea in rat model and explore its anti-diarrhea mechanism.

Methods:

Seventy-five Sprague-Dawley rats were divided into 5 groups by a random number table

including control

positive

model

low-dose (LD) and high-dose (HD) ESP groups

15 rats in each group. All the rats

except those in the control group

were developed PSYD induced-diarrhea based on its pathology and etiology. The rats in positive

LD and HD ESP groups were treated with Shenling Baizhu Pill (参苓白术丸)

LD (1.05 g/kg) or HD (3.50 g/kg) ESP petroleum ether extract once a day for 2 weeks

respectively. Body weight change and diarrhea index were measured. The histology scores of the kidney were evaluated via hematoxylin and eosin (HE) staining. Aquaporin-3 (AQP3) expression in the colon was analyzed by immunofluorescence

quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and Western blot

respectively.

Results:

Compared with the model group

oral administration of LD and HD ESP prevented body weight loss and inhibited diarrhea after 2-week treatment (

0.05). Kidney deterioration was impeded

and the histology score in LD and HD ESP groups were 8.2 and 10.5

respectively

which were both higher than those in the model group (

0.05). In addition

ESP treatment alleviated rat colitis

and HD ESP significantly improved the AQP3 positive staining intensity in the colon tissue compared with the model group. The result from Western blot revealed that AQP3 protein synthesis in colon tissue of LD and HD ESP groups increased by 2.1- and 5.9-fold compared with the model group (

0.05). qRT-PCR result showed that AQP3 gene expression in the HD ESP group was also up-regulated by 2.5-fold normalized to the model group (

0.05).

Conclusion:

ESP extract effectively alleviates the symptoms of PSYD and relieves PSYD-induced diarrhea by improving AQP3 synthesis in the colon.

关键词

Keywords

Ershen PillChinese medicinepetroleum ether extractanti-diarrheaAQP3colon

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