Scutellaria barbata D. Don inhibits colorectal cancer growth via suppression of Wnt/β-catenin signaling pathway

Li-hui Wei; Jiu-mao Lin; Jian-feng Chu; Hong-wei Chen; Qing-yu Li; Jun Peng

doi:10.1007/s11655-017-2775-3

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Scutellaria barbata D. Don inhibits colorectal cancer growth via suppression of Wnt/β-catenin signaling pathway

OriginalPaper | Updated：2021-08-27

- Scutellaria barbata D. Don inhibits colorectal cancer growth via suppression of Wnt/β-catenin signaling pathway
- Scutellaria barbata D. Don inhibits colorectal cancer growth via suppression of Wnt/β-catenin signaling pathway
- 中国结合医学杂志（英文版） 2017年23卷第11期页码：858-863
- Affiliations：
  
  1. Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine,Fuzhou,China
  2. Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese Medicine,Fuzhou,China
- Author bio：
- Funds：
  
  Supported by the Natural Science Foundation of Fujian Province of China (No. 2013J01333), the Youth Science Foundation of the Health Department of Fujian Province (2012-2-60) and the Developmental Fund of Chen Keji Integrative Medicine (No. CKJ2015008)
- DOI：10.1007/s11655-017-2775-3
  中图分类号：
- 纸质出版日期：2017，
  
  网络出版日期：2017-10-28，
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Wei, Lh., Lin, Jm., Chu, Jf. et al. Scutellaria barbata D. Don inhibits colorectal cancer growth via suppression of Wnt/β-catenin signaling pathway., Chin. J. Integr. Med. 23, 858–863 (2017). https://doi.org/10.1007/s11655-017-2775-3

Li-hui Wei, Jiu-mao Lin, Jian-feng Chu, et al. Scutellaria barbata D. Don inhibits colorectal cancer growth via suppression of Wnt/β-catenin signaling pathway[J]. Chinese Journal of Integrative Medicine, 2017,23(11):858-863.
Wei, Lh., Lin, Jm., Chu, Jf. et al. Scutellaria barbata D. Don inhibits colorectal cancer growth via suppression of Wnt/β-catenin signaling pathway., Chin. J. Integr. Med. 23, 858–863 (2017). https://doi.org/10.1007/s11655-017-2775-3 DOI：

Li-hui Wei, Jiu-mao Lin, Jian-feng Chu, et al. Scutellaria barbata D. Don inhibits colorectal cancer growth via suppression of Wnt/β-catenin signaling pathway[J]. Chinese Journal of Integrative Medicine, 2017,23(11):858-863. DOI： 10.1007/s11655-017-2775-3.

摘要

To investigate the effect of the ethanol extract of Scutellaria barbata D. Don (EESB) on colorectal cancer (CRC) growth and Wnt/β-catenin signaling pathway in vivo and in vitro. In vivo experiment

CRC xenograft mouse model was constructed with injection of HT-29 cells. Following xenograft implantation

twenty mice were randomly divided into EESB-treated group (n=10) and control group (n=10) by a random number table

and were given with intra-gastric administration of 2 g/kg EESB or saline

5 days a week for 16 days

respectively. At the end of experiment

tumors were removed and weighed by electronic scales. The proliferation biomarker Ki-67 of tumor was evaluated by immunohistochemistry (IHC) assay. In vitro study

HT-29 cells were treated with 0

0.5

1.5

2.5 mg/mL EESB for 24 h. At the end of the treatment

the viability and survival of HT-29 cells were determined by methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay and colony formation assay

respectively. The mRNA expression of c-Myc

Survivin and adenomatous polyposis coli (APC) was examined by reverse transcription-polymerase chain reaction (RT-PCR) both in tumor tissues of CRC xenograft mice and HT-29 cells. Protein expression of c-Myc

Survivin

APC

and β-catenin as well as β-catenin phosphorylation level were evaluated by IHC assay or Western blotting. EESB significantly reduced tumor weight in CRC xenografts mice

compared with the control group (P<0.05). IHC assay showed that EESB significantly inhibited protein expression of Ki-67 in tumor tissues (P<0.05). MTT assay showed that EESB significantly reduced HT-29 cell viability in a dose-dependent manner (P<0.05). Colony formation assay showed that EESB dose-dependently decreased the survival of HT-29 cells (P<0.05). In addition

RT-PCR assay showed that EESB decreased the mRNA expression of c-Myc and Survivin and increased APC expression

both in tumor tissues of CRC xenograft mice and HT-29 cells (P<0.05). IHC assay or Western blotting showed that EESB decreased protein expression of β-catenin

c-Myc and Survivin

as well as increased APC expression and β-catenin phosphorylation in tumor tissues or HT-29 cells (P<0.05). EESB significantly reduced tumor growth in CRC xenografts mice

and inhibited the viability and survival of HT-29 cells. EESB could suppress the activation of the Wnt/β-catenin pathway

which might be one of the mechanisms whereby Scutellaria barbata D. Don exerts its anticancer activity.

Abstract

To investigate the effect of the ethanol extract of Scutellaria barbata D. Don (EESB) on colorectal cancer (CRC) growth and Wnt/β-catenin signaling pathway in vivo and in vitro. In vivo experiment

CRC xenograft mouse model was constructed with injection of HT-29 cells. Following xenograft implantation

twenty mice were randomly divided into EESB-treated group (n=10) and control group (n=10) by a random number table

and were given with intra-gastric administration of 2 g/kg EESB or saline

5 days a week for 16 days

respectively. At the end of experiment

tumors were removed and weighed by electronic scales. The proliferation biomarker Ki-67 of tumor was evaluated by immunohistochemistry (IHC) assay. In vitro study

HT-29 cells were treated with 0

0.5

1.5

2.5 mg/mL EESB for 24 h. At the end of the treatment

the viability and survival of HT-29 cells were determined by methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay and colony formation assay

respectively. The mRNA expression of c-Myc

Survivin

APC

and β-catenin as well as β-catenin phosphorylation level were evaluated by IHC assay or Western blotting. EESB significantly reduced tumor weight in CRC xenografts mice

RT-PCR assay showed that EESB decreased the mRNA expression of c-Myc and Survivin and increased APC expression

both in tumor tissues of CRC xenograft mice and HT-29 cells (P<0.05). IHC assay or Western blotting showed that EESB decreased protein expression of β-catenin

c-Myc and Survivin

as well as increased APC expression and β-catenin phosphorylation in tumor tissues or HT-29 cells (P<0.05). EESB significantly reduced tumor growth in CRC xenografts mice

and inhibited the viability and survival of HT-29 cells. EESB could suppress the activation of the Wnt/β-catenin pathway

which might be one of the mechanisms whereby Scutellaria barbata D. Don exerts its anticancer activity.

关键词

Scutellaria barbata D. Doncolorectal cancerproliferationWnt/β-catenin pathway

Keywords

Scutellaria barbata D. Doncolorectal cancerproliferationWnt/β-catenin pathway

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