Antipyretic Effect of Herba Ephedrae-Ramulus Cinnamomi Herb Pair on Yeast-Induced Pyrexia Rats: A Metabolomics Study

Xiao-ming Wang; Wen-jie Xu; Liang-kui Xu; Shuai Song; Xue-feng Xing; Jia-bo Luo

doi:10.1007/s11655-017-2778-0

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Antipyretic Effect of Herba Ephedrae-Ramulus Cinnamomi Herb Pair on Yeast-Induced Pyrexia Rats: A Metabolomics Study

OriginalPaper | Updated：2021-08-27

- Antipyretic Effect of Herba Ephedrae-Ramulus Cinnamomi Herb Pair on Yeast-Induced Pyrexia Rats: A Metabolomics Study
- Antipyretic Effect of Herba Ephedrae-Ramulus Cinnamomi Herb Pair on Yeast-Induced Pyrexia Rats: A Metabolomics Study
- 中国结合医学杂志（英文版） 2018年24卷第9期页码：676-682
- Affiliations：
  
  1. School of Traditional Chinese Medical Sciences, Southern Medical University,Guangzhou,China
  2. School of Pharmacy, Guangdong Pharmaceutical University,Guangzhou,China
  3. Department of Traditional Chinese Medicine Pharmaceutics, Guangdong Second Traditional Chinese Medicine Hospital,Guangzhou,China
  4. Guangdong Provincial Key Laboratory of Research and Development of Traditional Chinese Medicine, Guangdong Province Engineering, Technology Research Institute of Traditional Chinese Medicine,Guangzhou,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81030066)
- DOI：10.1007/s11655-017-2778-0
  中图分类号：
- 纸质出版日期：2018，
  
  网络出版日期：2018-1-9，
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Wang, Xm., Xu, Wj., Xu, Lk. et al. Antipyretic Effect of Herba Ephedrae-Ramulus Cinnamomi Herb Pair on Yeast-Induced Pyrexia Rats: A Metabolomics Study., Chin. J. Integr. Med. 24, 676–682 (2018). https://doi.org/10.1007/s11655-017-2778-0

Xiao-ming Wang, Wen-jie Xu, Liang-kui Xu, et al. Antipyretic Effect of Herba Ephedrae-Ramulus Cinnamomi Herb Pair on Yeast-Induced Pyrexia Rats: A Metabolomics Study[J]. Chinese Journal of Integrative Medicine, 2018,24(9):676-682.
Wang, Xm., Xu, Wj., Xu, Lk. et al. Antipyretic Effect of Herba Ephedrae-Ramulus Cinnamomi Herb Pair on Yeast-Induced Pyrexia Rats: A Metabolomics Study., Chin. J. Integr. Med. 24, 676–682 (2018). https://doi.org/10.1007/s11655-017-2778-0 DOI：

Xiao-ming Wang, Wen-jie Xu, Liang-kui Xu, et al. Antipyretic Effect of Herba Ephedrae-Ramulus Cinnamomi Herb Pair on Yeast-Induced Pyrexia Rats: A Metabolomics Study[J]. Chinese Journal of Integrative Medicine, 2018,24(9):676-682. DOI： 10.1007/s11655-017-2778-0.

摘要

To investigate the antipyretic mechanism of Herba Ephedrae (Eph)-Ramulus Cinnamomi (RC) herb pair on yeast-induced pyrexia in rats. Totally 30 qualified male SD rats were randomly assigned to the normal control (NC) group

the pyrexia model (model) group

the Eph

RC and Eph-RC treatment groups by a random digital table

6 rats in each group. Each rat received a 20% aqueous suspension of yeast (10 mL/kg) except the NC group. The 3 treatment groups were administered 8.1

5.4 and 13.5 g/kg Eph

RC and Eph-RC respectively at 5 and 12 h after yeast injection

the NC group and the model groups were administered equal volume of distilled water. Rectal temperatures were measured at 0

24 and 30 h and urine was collected prior to yeast injection and at 6

and 36 h after yeast injection. Then urine metabolomic profiling by gas chromatography tandem mass spectrometry

coupled with multivariate statistical analysis and pattern recognition techniques were used to explore the antipyretic effects of Eph-RC. Partial least squares discriminate analysis was used to analyze the metabolomics dataset including classification and regression in metabolomics plot profiling. Compared with the NC group

rectal temperatures were significantly higher in the model group (P<0.01)

while 3 treatment groups decreased significantly compared with the model group (P<0.05 or P<0.01). Rectal temperatures of Eph-RC-treated rats started to go down at 6 h

and markedly decreased at 8

18 and 24 h (P<0.05 or P<0.01)

while those of the Eph and RC groups had decreased firstly at 8 h and were markedly lower at 12 h (P<0.05 or P<0.01). Seventeen potential biomarkers related to pyrexia were confirmed and identified

including pyruvic acid

L-phenylalanine

L-tyrosine

phenylacetic acid

hippuric acid

succinic acid

citrate and so on. Eight potential alterations of metabolic pathways including phenylalanine metabolism

citrate cycle

tryptophan metabolism

biosynthesis of valine

leucine and isoleucine

were identified in relation to the antipyretic effects of Eph-RC using MetPA software. The antipyretic effect of Eph-RC herb pair on yeast-induced pyrexia in rats involved correction of perturbed amino acid

fatty acid

and carbohydrate metabolism according to the metabolic pathway analysis with MetPA.

Abstract

the pyrexia model (model) group

the Eph

RC and Eph-RC treatment groups by a random digital table

6 rats in each group. Each rat received a 20% aqueous suspension of yeast (10 mL/kg) except the NC group. The 3 treatment groups were administered 8.1

5.4 and 13.5 g/kg Eph

RC and Eph-RC respectively at 5 and 12 h after yeast injection

the NC group and the model groups were administered equal volume of distilled water. Rectal temperatures were measured at 0

24 and 30 h and urine was collected prior to yeast injection and at 6

and 36 h after yeast injection. Then urine metabolomic profiling by gas chromatography tandem mass spectrometry

rectal temperatures were significantly higher in the model group (P<0.01)

while 3 treatment groups decreased significantly compared with the model group (P<0.05 or P<0.01). Rectal temperatures of Eph-RC-treated rats started to go down at 6 h

and markedly decreased at 8

18 and 24 h (P<0.05 or P<0.01)

while those of the Eph and RC groups had decreased firstly at 8 h and were markedly lower at 12 h (P<0.05 or P<0.01). Seventeen potential biomarkers related to pyrexia were confirmed and identified

including pyruvic acid

L-phenylalanine

L-tyrosine

phenylacetic acid

hippuric acid

succinic acid

citrate and so on. Eight potential alterations of metabolic pathways including phenylalanine metabolism

citrate cycle

tryptophan metabolism

biosynthesis of valine

leucine and isoleucine

fatty acid

and carbohydrate metabolism according to the metabolic pathway analysis with MetPA.

关键词

Chinese MedicineHerba EphedraeRamulus Cinnamomiherb pairprincipal component analysispartial least squares-discriminant analysisbiomarker

Keywords

Chinese MedicineHerba EphedraeRamulus Cinnamomiherb pairprincipal component analysispartial least squares-discriminant analysisbiomarker

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