Dampness-Heat Accelerates DMBA-Induced Mammary Tumors in Rats

Wei Dong; Xi-lan Tang; Guang-bin Shang; Guo-liang Xu; Wei-feng Zhu; Hong-ning Liu

doi:10.1007/s11655-017-2821-1

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Dampness-Heat Accelerates DMBA-Induced Mammary Tumors in Rats

OriginalPaper | Updated：2021-08-27

- Dampness-Heat Accelerates DMBA-Induced Mammary Tumors in Rats
- Dampness-Heat Accelerates DMBA-Induced Mammary Tumors in Rats
- 中国结合医学杂志（英文版） 2018年24卷第10期页码：758-762
- Affiliations：
  
  1. Key Laboratory of Modern Preparation of Traditional Chinese Medicine, Jiangxi University of Traditional Chinese Medicine,Nanchang,China
  2. Jiangxi Provincial Key Laboratory of Drug Design and Evaluation, School of Pharmacy, Jiangxi Science and Technology Normal University,Nanchang,China
  3. Research Center for Differentiation and Development of Basic Theory of Traditional Chinese Medicine, Jiangxi University of Traditional Chinese Medicine,Nanchang,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81260523)
- DOI：10.1007/s11655-017-2821-1
  中图分类号：
- 纸质出版日期：2018，
  
  网络出版日期：2017-9-15，
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Dong, W., Tang, Xl., Shang, Gb. et al. Dampness-Heat Accelerates DMBA-Induced Mammary Tumors in Rats., Chin. J. Integr. Med. 24, 758–762 (2018). https://doi.org/10.1007/s11655-017-2821-1

Wei Dong, Xi-lan Tang, Guang-bin Shang, et al. Dampness-Heat Accelerates DMBA-Induced Mammary Tumors in Rats[J]. Chinese Journal of Integrative Medicine, 2018,24(10):758-762.
Dong, W., Tang, Xl., Shang, Gb. et al. Dampness-Heat Accelerates DMBA-Induced Mammary Tumors in Rats., Chin. J. Integr. Med. 24, 758–762 (2018). https://doi.org/10.1007/s11655-017-2821-1 DOI：

Wei Dong, Xi-lan Tang, Guang-bin Shang, et al. Dampness-Heat Accelerates DMBA-Induced Mammary Tumors in Rats[J]. Chinese Journal of Integrative Medicine, 2018,24(10):758-762. DOI： 10.1007/s11655-017-2821-1.

摘要

To investigate the impact of dampness-heat (DH) on the development of mammary tumors in 7

12-dimethylbenz(a)anthracene (DMBA)-induced rats. Forty rats were randomly divided into 3 groups in a randomized block design

including the control group (n=13)

DMBA group (n=14)

and DMBA plus DH group (n=13). Rats in the DMBA group and DMBA plus DH group were intragastrically administrated with DMBA (100 mg/kg) for twice

once per week

while rats in the control group were treated with equivalent volumes of sesame oil. After DMBA administration

rats in the DMBA plus DH group were exposed to a simulated climate chamber with ambient temperature (33.0±0.5°C) and humidity (90%±5%) for 8 weeks

8 h per day. The body weight

time of tumor formation

and number of tumors were measured weekly to calculate tumor incidence

average latency period

average number of tumors

and average tumor weight. At the end of the experiment

the levels of matrix metalloproteinase 9 (MMP-9) and tissue inhibitor of metalloproteinases 1 (TIMP-1) in serum

and the contents of tumor necrosis factor-α (TNF-α) and interleukin (IL)-1β in serum and tumor tissue were measured

respectively. Some tumor tissues were processed for hematoxylin-eosin staining to determine the histopathological changes. Compared with DMBA

DMBA plus DH significantly increased the average number of tumors

average tumor weight

levels of serum MMP-9

TIMP-1

TNF-α and IL-1β

and contents of tumor tissue TNF-α and IL-1β (P<0.05 or P<0.01). DH could accelerate the development of mammary tumors through increasing the expressions of MMP-9

TIMP-1

TNF-α and IL-1β in DMBA-induced rats.

Abstract

To investigate the impact of dampness-heat (DH) on the development of mammary tumors in 7

12-dimethylbenz(a)anthracene (DMBA)-induced rats. Forty rats were randomly divided into 3 groups in a randomized block design

including the control group (n=13)

DMBA group (n=14)

and DMBA plus DH group (n=13). Rats in the DMBA group and DMBA plus DH group were intragastrically administrated with DMBA (100 mg/kg) for twice

once per week

while rats in the control group were treated with equivalent volumes of sesame oil. After DMBA administration

rats in the DMBA plus DH group were exposed to a simulated climate chamber with ambient temperature (33.0±0.5°C) and humidity (90%±5%) for 8 weeks

8 h per day. The body weight

time of tumor formation

and number of tumors were measured weekly to calculate tumor incidence

average latency period

average number of tumors

and average tumor weight. At the end of the experiment

the levels of matrix metalloproteinase 9 (MMP-9) and tissue inhibitor of metalloproteinases 1 (TIMP-1) in serum

and the contents of tumor necrosis factor-α (TNF-α) and interleukin (IL)-1β in serum and tumor tissue were measured

respectively. Some tumor tissues were processed for hematoxylin-eosin staining to determine the histopathological changes. Compared with DMBA

DMBA plus DH significantly increased the average number of tumors

average tumor weight

levels of serum MMP-9

TIMP-1

TNF-α and IL-1β

and contents of tumor tissue TNF-α and IL-1β (P<0.05 or P<0.01). DH could accelerate the development of mammary tumors through increasing the expressions of MMP-9

TIMP-1

TNF-α and IL-1β in DMBA-induced rats.

关键词

dampness-heatmammary tumor712-dimethylbenz(a)anthraceneinflammatory response

Keywords

dampness-heatmammary tumor712-dimethylbenz(a)anthraceneinflammatory response

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Intelligent Chinese Medicine: A New Direction Approach for Integrative Medicine in Diagnosis and Treatment of Cardiovascular Diseases