Embolic Effects of <italic style="font-style: italic">Bletilla striata</italic> Microspheres in Renal Artery and Transplanted VX2 Liver Tumor Model in Rabbits

Shi-hua LUO; Song-lin SONG; Chuan-sheng ZHENG; Wei-yong LI; Yong WANG; Xiang-wen XIA; Gan-sheng FENG

doi:10.1007/s11655-017-2953-3

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Embolic Effects of Bletilla striata Microspheres in Renal Artery and Transplanted VX2 Liver Tumor Model in Rabbits

Original Article | Updated：2021-08-27

- Embolic Effects of Bletilla striata Microspheres in Renal Artery and Transplanted VX2 Liver Tumor Model in Rabbits
- Embolic Effects of Bletilla striata Microspheres in Renal Artery and Transplanted VX2 Liver Tumor Model in Rabbits
- Chinese Journal of Integrative Medicine 2019年25卷第6期页码：431-438
- Affiliations：
  
  1.Department of Radiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan (430022), China
  2.Department of Pharmacy, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan (430022), China
- Author bio：
  
  Correspondence to: Prof. ZHENG Chuan-sheng, Tel: 86-27-85726432, E-mail: hqzcsxh@sina.com
- Funds：
  
  Department of Pharmacy, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology
- DOI：10.1007/s11655-017-2953-3
  中图分类号：
- 纸质出版日期：2019-06-01，
  
  网络出版日期：2017-05-11，
  
  录用日期：2016-07-26
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Shi-hua LUO, Song-lin SONG, Chuan-sheng ZHENG, 等. Embolic Effects of Bletilla striata Microspheres in Renal Artery and Transplanted VX2 Liver Tumor Model in Rabbits[J]. Chinese Journal of Integrative Medicine, 2019,25(6):431-438.

Shi-hua LUO, Song-lin SONG, Chuan-sheng ZHENG, et al. Embolic Effects of Bletilla striata Microspheres in Renal Artery and Transplanted VX2 Liver Tumor Model in Rabbits[J]. Chinese Journal of Integrative Medicine, 2019,25(6):431-438.
Shi-hua LUO, Song-lin SONG, Chuan-sheng ZHENG, 等. Embolic Effects of Bletilla striata Microspheres in Renal Artery and Transplanted VX2 Liver Tumor Model in Rabbits[J]. Chinese Journal of Integrative Medicine, 2019,25(6):431-438. DOI： 10.1007/s11655-017-2953-3.

Shi-hua LUO, Song-lin SONG, Chuan-sheng ZHENG, et al. Embolic Effects of Bletilla striata Microspheres in Renal Artery and Transplanted VX2 Liver Tumor Model in Rabbits[J]. Chinese Journal of Integrative Medicine, 2019,25(6):431-438. DOI： 10.1007/s11655-017-2953-3.

摘要

Abstract

Objective:

To evaluate the characteristics of

Bletilla striata

microspheres (BSMs) and its effects as an embolic agent in a rabbit model.

Methods:

BSMs were prepared with an emulsification-cool condensation-chemical cross-linking method. The characteristics of BSMs

in vitro

were observed. Embolization experiments were performed in renal artery of rabbit and in a rabbit liver VX2 carcinoma model. Seventy-two New Zealand rabbits were divided into 2 groups

and the right renal artery was embolized with BSMs (200 μm in diameter) in the experimental group and with polyvinyl alcohol (PVA) of the same size in the control group. The pathological findings were examined with hematoxylin-eosin and Masson stainings. Liver and renal functions were tested before and after embolization. VX2 tumor was transplanted in 15 New Zealand rabbits

which were randomly divided into 3 groups (

=5). Group A were treated with saline

group B with a mixture of doxorubicin and lipiodol

and group C with hepatic arterial infusion of BSMs (200 μm in diameter). Tumor growth rate was evaluated by magnetic resonance imaging scan. Apoptosis-related factors (bax

bcl-2) and tumor vascular endothelial cell growth factor (VEGF) were evaluated through immunohistochemical staining.

Results:

The characteristics of BSMs

in vitro

were in full compliance with the requirements for use in interventional procedures. In the renal artery embolization experiment

after BSMs intervention

it was more difficult to form collateral circulation than that with PVAs

and the kidney manifested atrophy and calcification. There were no significant difference of liver and renal functions in rabbits between groups. In the liver VX2 carcinoma embolization experiment

compared with group A

the growth rate of VX2 liver tumor and Bcl-2 levels was reduced

while apoptosis index

Bax

and VEGF were increased in group B (

0.05). There were no significant difference between groups B and C (

0.05).

Conclusions:

The characteristics of BSMs

in vitro

and

in vivo

meet the requirements for its use as an embolic agent in interventional approaches.

关键词

Keywords

Bletilla striataChinese medicinemicrospherecharacteristicembolization

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Embolic Effects of Bletilla striata Microspheres in Renal Artery and Transplanted VX2 Liver Tumor Model in Rabbits

Embolic Effects of Bletilla striata Microspheres in Renal Artery and Transplanted VX2 Liver Tumor Model in Rabbits

DOI：10.1007/s11655-017-2953-3

摘要

Abstract

关键词

Keywords

references