Xuebijing Injection (血必净注射液) and Resolvin D1 Synergize Regulate Leukocyte Adhesion and Improve Survival Rate in Mice with Sepsis-Induced Lung Injury

Shu-kun Zhang; Yu-zhen Zhuo; Cai-xia Li; Lei Yang; Hong-wei Gao; Xi-mo Wang

doi:10.1007/s11655-017-2959-x

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Xuebijing Injection (血必净注射液) and Resolvin D1 Synergize Regulate Leukocyte Adhesion and Improve Survival Rate in Mice with Sepsis-Induced Lung Injury

OriginalPaper | Updated：2021-08-27

- Xuebijing Injection (血必净注射液) and Resolvin D1 Synergize Regulate Leukocyte Adhesion and Improve Survival Rate in Mice with Sepsis-Induced Lung Injury
- Xuebijing Injection (血必净注射液) and Resolvin D1 Synergize Regulate Leukocyte Adhesion and Improve Survival Rate in Mice with Sepsis-Induced Lung Injury
- 中国结合医学杂志（英文版） 2018年24卷第4期页码：272-277
- Affiliations：
  
  1. Department of Cell and Molecular Biology, Institute of Acute Abdominal Diseases of Intergrated Traditional Chinese and Western Medicine, Tianjin Nankai Hospital,Tianjin,China
  2. Center for Experimental Therapeutics and Reperfusion Injury, Department of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women’s Hospital, Harvard Medical School,Boston,USA
  3. Department of Surgery, Tianjin Nankai Hospital,Tianjin,China
- Author bio：
- Funds：
- DOI：10.1007/s11655-017-2959-x
  中图分类号：
- 纸质出版日期：2018，
  
  网络出版日期：2017-5-17，
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Zhang, Sk., Zhuo, Yz., Li, Cx. et al. Xuebijing Injection (血必净注射液) and Resolvin D1 Synergize Regulate Leukocyte Adhesion and Improve Survival Rate in Mice with Sepsis-Induced Lung Injury., Chin. J. Integr. Med. 24, 272–277 (2018). https://doi.org/10.1007/s11655-017-2959-x

Shu-kun Zhang, Yu-zhen Zhuo, Cai-xia Li, et al. Xuebijing Injection (血必净注射液) and Resolvin D1 Synergize Regulate Leukocyte Adhesion and Improve Survival Rate in Mice with Sepsis-Induced Lung Injury[J]. Chinese Journal of Integrative Medicine, 2018,24(4):272-277.
Zhang, Sk., Zhuo, Yz., Li, Cx. et al. Xuebijing Injection (血必净注射液) and Resolvin D1 Synergize Regulate Leukocyte Adhesion and Improve Survival Rate in Mice with Sepsis-Induced Lung Injury., Chin. J. Integr. Med. 24, 272–277 (2018). https://doi.org/10.1007/s11655-017-2959-x DOI：

Shu-kun Zhang, Yu-zhen Zhuo, Cai-xia Li, et al. Xuebijing Injection (血必净注射液) and Resolvin D1 Synergize Regulate Leukocyte Adhesion and Improve Survival Rate in Mice with Sepsis-Induced Lung Injury[J]. Chinese Journal of Integrative Medicine, 2018,24(4):272-277. DOI： 10.1007/s11655-017-2959-x.

摘要

To investigate the effect of combined application of Xuebijing Injection (血必净注射液

XBJ) and resolvin D1 (RvD1) on survival rate and the underlying mechanisms in mice with sepsisinduced lung injury. The cecal ligation and puncture (CLP) method was used to develop a mouse sepsis model. Specific pathogen free male C57BL/6 mice were randomly divided into 5 groups (n=20 each): sham

CLP

CLP+XBJ

CLP+RvD1 and CLP+XBJ+RvD1. After surgery

mice in the CLP+XBJ

CLP+RvD1 and CLP+XBJ+RvD1 groups were given XBJ (25 μL/g body weight)

RvD1 (10 ng/g body weight)

and their combination (the same dose of XBJ and RvD1)

respectively. In each group

12 mice were used to observe 1-week survival rate

while the rest were executed at 12 h. Whole blood was collected for flow cytometric analysis of leukocyte adhesion molecules CD18

lung tissues were harvested for observing pathological changes

and testing the activity of myeloperoxidase (MPO) and the expression of intercellular cell adhesion molecule 1 (ICAM-1). Compared with the CLP group

the histopathological damage of the lung tissues was mitigated

MPO activity was decreased in the CLP+XBJ and CLP+RvD1 groups (P<0.05). In addition

the 1-week survival rate was improved

proportion of CD18-expressing cells in whole blood and ICAM-1 protein expression in lung tissue were decreased in the CLP+XBJ+RvD1 group (P<0.05 or P<0.01). XBJ together with RvD1 could effectively inhibit leukocyte adhesion

reduce lung injury

and improve the survival rate of mice with sepsis.

Abstract

To investigate the effect of combined application of Xuebijing Injection (血必净注射液

CLP

CLP+XBJ

CLP+RvD1 and CLP+XBJ+RvD1. After surgery

mice in the CLP+XBJ

CLP+RvD1 and CLP+XBJ+RvD1 groups were given XBJ (25 μL/g body weight)

RvD1 (10 ng/g body weight)

and their combination (the same dose of XBJ and RvD1)

respectively. In each group

12 mice were used to observe 1-week survival rate

while the rest were executed at 12 h. Whole blood was collected for flow cytometric analysis of leukocyte adhesion molecules CD18

lung tissues were harvested for observing pathological changes

and testing the activity of myeloperoxidase (MPO) and the expression of intercellular cell adhesion molecule 1 (ICAM-1). Compared with the CLP group

the histopathological damage of the lung tissues was mitigated

MPO activity was decreased in the CLP+XBJ and CLP+RvD1 groups (P<0.05). In addition

the 1-week survival rate was improved

reduce lung injury

and improve the survival rate of mice with sepsis.

关键词

Xuebijing InjectionChinese Medicineresolvin D1sepsislung injury

Keywords

Xuebijing InjectionChinese Medicineresolvin D1sepsislung injury

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