Genomewide DNA Methylation Responses in Patients with β-Thalassemia Treated with Yisui Shengxue Granules (益髓生血颗粒)

Yan-ling CHENG; Xin-hua ZHANG; Yu-wen SUN; Wen-juan WANG; Jie HUANG; Na-li CHU; Su-ping FANG; Zhi-kui WU

doi:10.1007/s11655-018-2777-9

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Genomewide DNA Methylation Responses in Patients with β-Thalassemia Treated with Yisui Shengxue Granules (益髓生血颗粒)

Original Article | Updated：2021-08-27

- Genomewide DNA Methylation Responses in Patients with β-Thalassemia Treated with Yisui Shengxue Granules (益髓生血颗粒)
- Genomewide DNA Methylation Responses in Patients with β-Thalassemia Treated with Yisui Shengxue Granules (益髓生血颗粒)
- Chinese Journal of Integrative Medicine 2019年25卷第7期页码：490-496
- Affiliations：
  
  1.Molecular Biology Labaratory, South of Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing (102618), China
  2.Department of Hematology, 303rd Hospital of People's Liberatory Army, Nanning (530021), China
  3.Molecular Biology Laboratory of Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing (100053), China
  4.School of Traditional Chinese Medicine, Capital Medical University, Beijing (100069), China
- Author bio：
  
  Correspondence to: Prof. WU Zhi-kui, Tel: 86-10-88001149, E-mail: gamwuzhikui@sina.com
- Funds：
  
  the National Basic Research Program of China(2010CB530406);National Natural Science Foundation of China(81173167);the Natural Science Foundation of Guangxi(2012GXNSFAA053156)
- DOI：10.1007/s11655-018-2777-9
  中图分类号：
- 纸质出版日期：2019-07-01，
  
  网络出版日期：2018-05-03，
  
  录用日期：2017-01-09
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Yan-ling CHENG, Xin-hua ZHANG, Yu-wen SUN, 等. Genomewide DNA Methylation Responses in Patients with β-Thalassemia Treated with Yisui Shengxue Granules (益髓生血颗粒)[J]. Chinese Journal of Integrative Medicine, 2019,25(7):490-496.

Yan-ling CHENG, Xin-hua ZHANG, Yu-wen SUN, et al. Genomewide DNA Methylation Responses in Patients with β-Thalassemia Treated with Yisui Shengxue Granules (益髓生血颗粒)[J]. Chinese Journal of Integrative Medicine, 2019,25(7):490-496.
Yan-ling CHENG, Xin-hua ZHANG, Yu-wen SUN, 等. Genomewide DNA Methylation Responses in Patients with β-Thalassemia Treated with Yisui Shengxue Granules (益髓生血颗粒)[J]. Chinese Journal of Integrative Medicine, 2019,25(7):490-496. DOI： 10.1007/s11655-018-2777-9.

Yan-ling CHENG, Xin-hua ZHANG, Yu-wen SUN, et al. Genomewide DNA Methylation Responses in Patients with β-Thalassemia Treated with Yisui Shengxue Granules (益髓生血颗粒)[J]. Chinese Journal of Integrative Medicine, 2019,25(7):490-496. DOI： 10.1007/s11655-018-2777-9.

摘要

Abstract

Objective:

To examine the clinical effects of Yisui Shengxue Granules (益髓生血颗粒) in the treatment of β-thalassemia and explore its mechanism on DNA methylation levels.

Methods:

A randomized placebo-controlled double-blinded trial was conducted. Forty patients with β-thalassemia were recruited and distributed randomly by envelope method into an experimental group and a control group

20 patients in each group. The patients were given Yisui Shengxue Granules in the experimental group and placebo in the control group (12 g/bag

3 times a day) during a 3-month intervention. Before and after 1

and 3 months of treatment

peripheral intravenous blood was sampled

and blood parameters such as hemoglobin (Hb)

red blood cells (RBCs)

reticulocytes (Ret)

and fetal hemoglobin (HbF) were analyzed. Mononuclear cells from 5 patients

who showed an obvious treatment effect

were isolated by density gradient centrifugation. DNA methylation was analyzed using an Affymetrix USA GeneChip Human Promoter 1.0 Array and Input-promoter 1.0.

Results:

Compared with pre-treatment

there was an obvious increase in Hb and RBCs counts after 1

and 3 months in the experiment group (

0.01 or

0.05). Meanwhile

HbF increased from the 2nd to the 3rd month (

0.05). In the control group

Hb and RBCs showed no obvioas change. After 3-month treatment

DNA methylation results from 5 patients revealed that there were 24 hypomethylated genes and 3

685 hypermethylated genes compared with pre-treatment. Genes of insulin-like growth factor 1 receptor (IGF1R) and Janus kinase 3 (JAK3) revealed the most relations with other genes (degree: 21) and genes of 1-phosphatidylinositol-4

5-bisphosphate phosphodiesterase gamma 2 (PLCG2) and mitogen-activated protein kinase 10 (MAPK10) showed a stronger intermediary role (betweenness centrality=0.04).

Conclusions:

JAK3 and MAPK10 are two key genes in bone marrow and the lymphatic system

and JAK3 is likely to be related to hematopoietic cytokines in the process of early hematopoiesis. (Registration No. NCT01549080)

关键词

Keywords

β-thalassemiaYisui Shengxue GranuleChinese medicineDNA-methylation

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