Puerarin Up-regulates Methyl-CpG Binding Protein 2 Phosphorylation in Hippocampus of Vascular Dementia Rats

Hu-qing Wang; Meng Zhang; Jia-xin Zhao; Hai-qin Wu; Zhen Gao; Gui-lian Zhang; Ru Zhang

doi:10.1007/s11655-018-2822-0

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Puerarin Up-regulates Methyl-CpG Binding Protein 2 Phosphorylation in Hippocampus of Vascular Dementia Rats

OriginalPaper | Updated：2021-08-27

- Puerarin Up-regulates Methyl-CpG Binding Protein 2 Phosphorylation in Hippocampus of Vascular Dementia Rats
- Puerarin Up-regulates Methyl-CpG Binding Protein 2 Phosphorylation in Hippocampus of Vascular Dementia Rats
- 中国结合医学杂志（英文版） 2018年24卷第5期页码：372-377
- Affiliations：
  
  Department of Neurology, the Second Affiliated Hospital of Xi’an Jiaotong University,Xi’an,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81270415/H2501)
- DOI：10.1007/s11655-018-2822-0
  中图分类号：
- 纸质出版日期：2018，
  
  网络出版日期：2018-1-9，
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Wang, Hq., Zhang, M., Zhao, Jx. et al. Puerarin Up-regulates Methyl-CpG Binding Protein 2 Phosphorylation in Hippocampus of Vascular Dementia Rats., Chin. J. Integr. Med. 24, 372–377 (2018). https://doi.org/10.1007/s11655-018-2822-0

Hu-qing Wang, Meng Zhang, Jia-xin Zhao, et al. Puerarin Up-regulates Methyl-CpG Binding Protein 2 Phosphorylation in Hippocampus of Vascular Dementia Rats[J]. Chinese Journal of Integrative Medicine, 2018,24(5):372-377.
Wang, Hq., Zhang, M., Zhao, Jx. et al. Puerarin Up-regulates Methyl-CpG Binding Protein 2 Phosphorylation in Hippocampus of Vascular Dementia Rats., Chin. J. Integr. Med. 24, 372–377 (2018). https://doi.org/10.1007/s11655-018-2822-0 DOI：

Hu-qing Wang, Meng Zhang, Jia-xin Zhao, et al. Puerarin Up-regulates Methyl-CpG Binding Protein 2 Phosphorylation in Hippocampus of Vascular Dementia Rats[J]. Chinese Journal of Integrative Medicine, 2018,24(5):372-377. DOI： 10.1007/s11655-018-2822-0.

摘要

To observe the effect of puerarin on methyl-CpG binding protein 2 (MeCP2) phosphorylation (pMeCP2) in the hippocampus of a rat model of vascular dementia (VD). Thirty-six healthy Sprague-Dawley rats were randomly assigned to the sham-operated group

dementia group and puerarintreated group using a random number table (n=12 per group). The modifified permanent bilateral common carotid artery occlusion method was used to establish the VD model. The sham-operated and dementia groups were given 2 mL/d of saline

while the puerarin-treated group was given 100 mg/(kg•d) of puerarin for 17 days. The learning and memory abilities were evaluated by the Morris water maze test. Hematoxylin-eosin staining

immunohistochemical (IHC) staining and Western blot analysis were carried out to observe changes in neuron morphology and in level of pMeCP2 in the hippocampus

respectively. The morphologies of rat hippocampal neurons in the puerarintreated group were markedly improved compared with the dementia group. The escape latency of the dementia group was significantly longer than the sham-operated group (P<0.05)

while the puerarin-treated group was obviously shorter than the dementia group (P<0.05). Cross-platform times of the dementia group were signifificantly decreased compared with the sham-operated group (P<0.05)

while the puerarin-treated group was obviously increased compared with the dementia group (P<0.05). IHC staining showed no significant difference in the number of MeCP2 positive cells among 3 groups (P>0.05). The number of pMeCP2 positive cells in the CA1 region of hippocampus in the dementia group was signifificantly increased compared with the sham-operated group

and the puerarin-treated group was signifificantly increased compared with the dementia group (both P<0.05). Western blot analysis showed no signifificant difference of MeCP2 expression among 3 groups (P>0.05). The expression of pMeCP2 in the dementia group was signifificantly increased compared with the sham-operated group

while it in the puerarin-treated group was signifificantly increased compared with the dementia group (P<0.05). Puerarin could play a role in the protection of nerve cells through up-regulating pMeCP2 in the hippocampus

improving neuron morphologies

and enhancing learning and memory ablities in a rat model of VD.

Abstract

while the puerarin-treated group was given 100 mg/(kg•d) of puerarin for 17 days. The learning and memory abilities were evaluated by the Morris water maze test. Hematoxylin-eosin staining

immunohistochemical (IHC) staining and Western blot analysis were carried out to observe changes in neuron morphology and in level of pMeCP2 in the hippocampus

improving neuron morphologies

and enhancing learning and memory ablities in a rat model of VD.

关键词

Vascular DementiaPuerarinmethyl-CpG binding protein 2phosphorylation

Keywords

Vascular DementiaPuerarinmethyl-CpG binding protein 2phosphorylation

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