Effects of Heyan Kuntai Capsule (和颜坤泰胶囊) on Follicular Development and Oocyte Cohesin Levels in Aged Mice

Bin Zhang; Nan Chu; Xue-min Qiu; Wei Tang; Hans-Jürgen Gober; Da-jin Li; Ling Wang

doi:10.1007/s11655-018-2835-3

Your Location：

Home >

Browse articles >

Effects of Heyan Kuntai Capsule (和颜坤泰胶囊) on Follicular Development and Oocyte Cohesin Levels in Aged Mice

OriginalPaper | Updated：2021-08-27

- Effects of Heyan Kuntai Capsule (和颜坤泰胶囊) on Follicular Development and Oocyte Cohesin Levels in Aged Mice
- Effects of Heyan Kuntai Capsule (和颜坤泰胶囊) on Follicular Development and Oocyte Cohesin Levels in Aged Mice
- 中国结合医学杂志（英文版） 2018年24卷第10期页码：768-776
- Affiliations：
  
  1. Institute of Obstetrics and Gynecology, Obstetrics and Gynecology Hospital of Fudan University,Shanghai,China
  2. The Academy of Integrative Medicine of Fudan University,Shanghai,China
  3. Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases,Shanghai,China
  4. Hepato-Biliary-Pancreatic Surgery Division, Department of Surgery, Graduate School of Medicine, the University of Tokyo,Tokyo,Japan
  5. Department of Pharmacy, Kepler University Clinic, Neuromed Campus, Linz,Austria
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 31571196, 81401171, 30801502), the 2015 Program to Guide Medicine of the Shanghai Municipal Science and Technology Commission (No. 15401932200), the FY2008 JSPS Postdoctoral Fellowship for Foreign Researchers (No. P08471), the Shanghai Pujiang Program (No. 11PJ1401900), the Development Project of Shanghai Peak Disciplines-Integrated Chinese and Western Medicine, the Program for Outstanding Medical Academic Leader, and Development Project of Shanghai Peak Disciplines-Integrated Chinese and Western Medicine (No. 20150407)
- DOI：10.1007/s11655-018-2835-3
  中图分类号：
- 纸质出版日期：2018，
  
  网络出版日期：2018-4-17，
Scan for full text
Zhang, B., Chu, N., Qiu, Xm. et al. Effects of Heyan Kuntai Capsule (和颜坤泰胶囊) on Follicular Development and Oocyte Cohesin Levels in Aged Mice., Chin. J. Integr. Med. 24, 768–776 (2018). https://doi.org/10.1007/s11655-018-2835-3

Bin Zhang, Nan Chu, Xue-min Qiu, et al. Effects of Heyan Kuntai Capsule (和颜坤泰胶囊) on Follicular Development and Oocyte Cohesin Levels in Aged Mice[J]. Chinese Journal of Integrative Medicine, 2018,24(10):768-776.
Zhang, B., Chu, N., Qiu, Xm. et al. Effects of Heyan Kuntai Capsule (和颜坤泰胶囊) on Follicular Development and Oocyte Cohesin Levels in Aged Mice., Chin. J. Integr. Med. 24, 768–776 (2018). https://doi.org/10.1007/s11655-018-2835-3 DOI：

Bin Zhang, Nan Chu, Xue-min Qiu, et al. Effects of Heyan Kuntai Capsule (和颜坤泰胶囊) on Follicular Development and Oocyte Cohesin Levels in Aged Mice[J]. Chinese Journal of Integrative Medicine, 2018,24(10):768-776. DOI： 10.1007/s11655-018-2835-3.

摘要

To evaluate the effect of Heyan Kuntai Capsule (和颜坤泰胶囊

HYKT) on the ovarian function of aged mice and expressions of cohesion complexes in oocytes. Twenty-five 9-month-old female C57BL/6J mice were randomly divided into 5 groups by block randomization method (n=5 per group)

including the control group (saline)

17β-estradiol group [E2

100 μg/(kg•d)]

and low-

medium-

and highdose of HYKT groups [0.3

0.9

2.7 g/(kg•d)

respectively]. All mice were treated by intragastric administration for 4 weeks. Hematoxylin and eosin staining and anti-VASA staining were used to detect the amounts of follicles. The apoptosis of follicles was measured by anti-gamma H2A histone family member X (γH2AX) staining and TdT-mediated dUTP Nick-End Labeling (TUNEL) assay. The density of cohesin subunits

REC8 meiotic recombination protein (REC8)

structural maintenance of chromosome (SMC) 1β and SMC3 in oocytes were evaluated by immunofluorescent staining. After administration of E2 and high-dose of HYKT

the total number of follicles as well as the number of primordial and primary follicles were significantly increased (P<0.05). Anti-γH2AX staining and TUNEL assay demonstrated that high-dose of HYKT and E2 partly suppressed the apoptosis of follicles (P<0.05). Furthermore

it showed an increased trend in the levels of REC8 and SMC1β

after administration with E2 and HYKT

and no obvious change in the level of SMC3. HYKT could enhance the number of follicles

suppress apoptosis of oocytes and have a trend to elevate the meiotic-specific cohesin subunits (REC8 and SMC1β) in oocytes of aged mice

indicating a beneficial effect on the ovarian function in terms of the quantity and quality of follicles.

Abstract

To evaluate the effect of Heyan Kuntai Capsule (和颜坤泰胶囊

including the control group (saline)

17β-estradiol group [E2

100 μg/(kg•d)]

and low-

medium-

and highdose of HYKT groups [0.3

0.9

2.7 g/(kg•d)

REC8 meiotic recombination protein (REC8)

structural maintenance of chromosome (SMC) 1β and SMC3 in oocytes were evaluated by immunofluorescent staining. After administration of E2 and high-dose of HYKT

it showed an increased trend in the levels of REC8 and SMC1β

after administration with E2 and HYKT

and no obvious change in the level of SMC3. HYKT could enhance the number of follicles

suppress apoptosis of oocytes and have a trend to elevate the meiotic-specific cohesin subunits (REC8 and SMC1β) in oocytes of aged mice

indicating a beneficial effect on the ovarian function in terms of the quantity and quality of follicles.

关键词

Follicular DevelopmentcohesinoocyteHeyan Kuntai CapsuleChinese Medicine

Keywords

Follicular DevelopmentcohesinoocyteHeyan Kuntai CapsuleChinese Medicine

references

Te Velde ER, Pearson PL. The variability of female reproductive ageing. Hum Reprod Update 2002;8:141–154.

Group ECW. Fertility and ageing. Hum Reprod Update 2005;11:261–276.

Ottolenghi C, Uda M, Hamatani T, Crisponi L, Garcia J-E, Ko M, et al. Aging of oocyte, ovary, and human reproduction. Annals New York Acad Sci 2004;1034:117–131.

Broekmans FJ, Knauff EaH, Te Velde ER, Macklon NS, Fauser BC. Female reproductive ageing: current knowledge and future trends. Trends Endocrinol Metabol 2007;18:58–65.

Broer SL, Broekmans FJM, Laven JSE, Fauser BCJM. Anti-Müllerian hormone: ovarian reserve testing and its potential clinical implications. Hum Reprod Update 2014;20:688–701.

Tilly JL, Kowalski KI, Johnson AL, Hsueh AJW. Involvement of apoptosis in ovarian follicular atresia and postovulatory regression. Endocrinology 1991;129:2799–2801.

Jiang JY, Cheung CKM, Wang Y, Tsang BK. Regulation of cell death and cell survival gene expression during ovarian follicular development and atresia. Front Biosci 2003;8:d222–d237.

Craig J, Orisaka M, Wang H, Orisaka S, Thompson W, Zhu C, et al. Gonadotropin and intra-ovarian signals regulating follicle development and atresia: the delicate balance between life and death. Front Biosci 2007;12:3628–3639.

Tatone C, Amicarelli F, Carbone MC, Monteleone P, Caserta D, Marci R, et al. Cellular and molecular aspects of ovarian follicle ageing. Hum Reprod Update 2008;14:131–142.

Faddy MJ, Gosden RG, Gougeon A, Richardson SJ, Nelson JF. Accelerated disappearance of ovarian follicles in mid-life: implications for forecasting menopause. Hum Reprod 1992;7:1342–1346.

Tilly JL. Commuting the death sentence: how oocytes strive to survive. Nat Rev Mol Cell Biol 2001;2:838–848.

Fujino Y, Ozaki K, Yamamasu S, Ito F, Matsuoka I, Hayashi E, et al. Ovary and ovulation: DNA fragmentation of oocytes in aged mice. Hum Reprod 1996;11:1480–1483.

Tatone C, Carbone MC, Gallo R, Delle Monache S, Di Cola M, Alesse E, et al. Age-associated changes in mouse oocytes during postovulatory in vitro culture: possible role for meiotic kinases and survival factor Bcl2. Biol Reprod 2006;74:395–402.

Hassold T, Hunt P. To err (meiotically) is human: the genesis of human aneuploidy. Nat Rev Genet 2001;2:280–291.

Nagaoka SI, Hassold TJ, Hunt PA. Human aneuploidy: mechanisms and new insights into an age-old problem. Nat Rev Genet 2012;13:493–504.

Haering CH, Löwe J, Hochwagen A, Nasmyth K. Molecular architecture of SMC proteins and the yeast cohesin complex. Mol Cell 2002;9:773–788.

Parisi S, Mckay MJ, Molnar M, Thompson MA, Van Der Spek PJ, et al. REC8p, a meiotic recombination and sister chromatid cohesion phosphoprotein of the Rad21p family conserved from fission yeast to humans. Mol Cell Biol 1999;19:3515–3528.

Watanabe Y, Nurse P. Cohesin REC8 is required for reductional chromosome segregation at meiosis. Nature 1999;400:461–464.

Pezzi N, Prieto I, Kremer L, Pérez Jurado LA, Valero C, Del Mazo J, et al. STAG3, a novel gene encoding a protein involved in meiotic chromosome pairing and location of STAG3-related genes flanking the Williams-Beuren syndrome deletion. FASEB J 2000;14:581–592.

Prieto I, Suja JA, Pezzi N, Kremer L, Martínez-A C, Rufas JS, et al. Mammalian STAG3 is a cohesin specific to sister chromatid arms in meiosis?. Nat Cell Biol 2001;3:761–766.

Revenkova E, Eijpe M, Heyting C, Gross B, Jessberger R. Novel meiosis-specific isoform of mammalian SMC1. Mol Cell Biol 2001;21:6984–6998.

Chiang T, Schultz RM, Lampson MA. Meiotic origins of maternal age-related aneuploidy. Biol Reprod 2012;86:1–7.

Jessberger R. Age-related aneuploidy through cohesion exhaustion. EMBO Reports 2012;13:539–546.

Jones KT, Lane SIR. Chromosomal, metabolic, environmental, and hormonal origins of aneuploidy in mammalian oocytes. Exp Cell Res 2012;318:1394–1399.

Herbert M, Kalleas D, Cooney D, Lamb M, Lister L. Meiosis and maternal aging: insights from aneuploid oocytes and trisomy births. Cold Spring Harbor Perspect Biol 2015;7:a017970.

Yang YF. RP-HPLC determination of paeoniflorin, baicalin, palmatine hydrochloride and berberine hydrochloride in Kuntai Capsules. Chin Traditional Patent Med (Chin) 2010;32:958–960.

Li HF. Simultaneous determination of 6 components in Kuntai Capsule by RP-HPLC wavelength switching method. China Mod Med (Chin) 2015;22:7–11.

Zhang QY, Wang JW, Yu JM. Efficacy and safety of Kuntai Capsule and estrogen for menopausal syndrome: a metaanalysis. Natl Med J China (Chin) 2013;93:3445–3449.

Li CC, Wang JJ, Chen C, Li YF, Zheng QS, Yang J, et al. Treating menopause syndrome by Kuntai Capsule and hormone replacement therapy: a meta-analysis of efficacy and safety comparison. Chin J Integr Tradit West Med (Chin) 2013;33:1183–1190.

Zhang SF, Liu Y, Xie Q, Bi CP, Zhu HT, Ha LX, et al. Effects of Gengnian Ningxin Capsule on the histomorphology of ovarin and uterine in the menopausal rats. Chin J Geriatr (Chin) 2004;23:648–651.

Lian F, Jiang XY. Effect of Kuntai Capsule on the number of retrieved oocytes, high-quality oocytes and embryos in in vitro fertilization of poor ovarian response patients. Chin J Integr Tradit West Med (Chin) 2014;34:917–921.

Li WW, An LH, Zhang G, Wang XL, Sun LJ, Guan YC, et al. The Combined application of estradiol valerate-cyproterone acetate and Kuntai Capsule in poor ovarian response patients in vitro fertilization. J Pract Obestet Gynecol (Chin) 2014;30:681–685.

Chang EM, Han JE, Won HJ, Kim YS, Yoon TK, Lee WS. Effect of estrogen priming through luteal phase and stimulation phase in poor responders in in-vitro fertilization. J Assist Reprod Genet 2012;29:225–230.

Wang CC, Li L, Tang LY, Leung PC. Safety evaluation of commonly used Chinese herbal medicines during pregnancy in mice. Hum Reprod 2012;27:2448–2456.

You SL, ed. Experimental zoology of obstetrics and gynecology. Beijing: Chinese Medicine Publisher of China; 2007:7–8.

Gougeon A. Regulation of ovarian follicular development in primates: facts and hypotheses. Endocr Rev 1996;17:121–55.

Skaznik-Wikiel ME, Mcguire MM, Sukhwani M, Donohue J, Chu T, Krivak TC, et al. Granulocyte colony-stimulating factor with or without stem cell factor extends time to premature ovarian insufficiency in female mice treated with alkylating chemotherapy. Fertil Steril 2013;99:2045–2054.

Zhang JM, Li LX, Yang YX, Liu XL, Wan XP. Is caspase inhibition a valid therapeutic strategy in cryopreservation of ovarian tissue? J Assist Reprod Genet 2009;26:415–420.

Tsutsumi M, Fujiwara R, Nishizawa H, Ito M, Kogo H, Inagaki H, et al. Age-related decrease of meiotic cohesins in human oocytes. PLoS One 2014;9:e96710.

Baker TG. A quantitative and cytological study of germ cells in human ovaries. Proceedings of the Royal Society of London. Series B, Biological Sciences 1963;158:417–433.

Billig H, Chun S, Eisenhauer K, Hsueh A. Gonadal cell apoptosis: hormone-regulated cell demise. Hum Reprod Update 1996;2:103–117.

Ansari RM. Potential use of durian fruit (Durio zibenthinus Linn) as an adjunct to treat infertility in polycystic ovarian syndrome. J Integr Med 2016;14:22–28.

Koebele SV, Bimonte-Nelson HA. Modeling menopause: the utility of rodents in translational behavioral endocrinology research. Maturitas 2016;87:5–17.

Albamonte MS, Willis MA, Albamonte MI, Jensen F, Espinosa MB, Vitullo AD. The developing human ovary: immunohistochemical analysis of germ-cell-specific VASA protein, BCL-2/BAX expression balance and apoptosis. Hum Reprod 2008;23:1895–1901.

Dutta S, Sengupta P. Men and mice: relating their ages. Life Sci 2016;152:244–248.

Roti Roti EC, Leisman SK, Abbott DH, Salih SM. Acute doxorubicin insult in the mouse ovary is cell- and follicletype dependent. PLoS One 2012;7:e42293.

Zhang SF, Ha LX, Xie Q, Jiang J, Liu Y, Xia X, et al. Effect of Gengnian Ningxin Capsule and estradial valerate on splenocyte apoptosis gene Bcl-2 and Bax expression of menopausal rats. Chin J Integr Tradit West Med (Chin) 2004;24:201–204.

Cun-Cun L, Jing-Jing W, Chao C, Yun-Fei L, Qing-Shan Z, Juan Y, et al. Clinical efficacy of Kuntai Capsule compared with estrogen progesterone sequential treatment on perimenopausal syndrome and impact on life quality. Chin J Biochem Pharm (Chin) 2013;33:1183–1190.

Garcia-Cruz R, Brieño MA, Roig I, Großsmann M, Velilla E, Pujol A, et al. Dynamics of cohesin proteins REC8, STAG3, SMC1ß and SMC3 are consistent with a role in sister chromatid cohesion during meiosis in human oocytes. Hum Reprod 2010;25:2316–2327.

Chiang T, Duncan FE, Schindler K, Schultz RM, Lampson MA. Evidence that weakened centromere cohesion is a leading cause of age-related aneuploidy in oocytes. Curr Biol 2010;20:1522–1528.

Lister LM, Kouznetsova A, Hyslop LA, Kalleas D, Pace SL, Barel JC, et al. Age-related meiotic segregation errors in mammalian oocytes are preceded by depletion of cohesin and Sgo2. Current Biol 2010;20:1511–1521.

Chiang T, Schultz RM, Lampson MA. Age-dependent susceptibility of chromosome cohesion to premature separase activation in mouse oocytes. Biol Reprod 2011;85:1279–1283.

Merriman JA, Jennings PC, Mclaughlin EA, Jones KT. Effect of aging on superovulation efficiency, aneuploidy rates, and sister chromatid cohesion in mice aged up to 15 months. Biol Reprod 2012;86:49.

Revenkova E, Eijpe M, Heyting C, Hodges CA, Hunt PA, Liebe B, et al. Cohesin SMC1ß is required for meiotic chromosome dynamics, sister chromatid cohesion and DNA recombination. Nat Cell Biol 2004;6:555–562.

Hodges CA, Revenkova E, Jeßsberger R, Hassold TJ, Hunt PA. SMC1ß-deficient female mice provide evidence that cohesins are a missing link in age-related nondisjunction. Nat Genet 2005;37:1351–1355.

Koehler KE, Schrump SE, Cherry JP, Hassold TJ, Hunt PA. Near-human aneuploidy levels in female mice with homeologous chromosomes. Current Biol 2006;16:R579–R580.

Selesniemi K, Lee HJ, Muhlhauser A, Tilly JL. Prevention of maternal aging-associated oocyte aneuploidy and meiotic spindle defects in mice by dietary and genetic strategies. Proc Natl Acad Sci U S A 2011;108:12319–12324.

FULL TEXT LINK

More>

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

A Multi-center, Randomized, Controlled and Open Clinical Trial of Heyan Kuntai Capsule (和颜坤泰胶囊) and Hormone Therapy in Perimenopausal Women

Future view and development of immunology: Exploring the immunology based on Chinese medicine and culture

Cost-effectiveness analysis of combined Chinese medicine and Western medicine for ischemic stroke patients

Objective tongue inspection on 142 liver cancer patients with damp-heat syndrome

Effect of Ermiao Recipe (二妙方) with medicinal guide Angelicae Pubescentis Radix on promoting the homing of bone marrow stem cells to treat cartilage damage in osteoarthritis rats