Buyang Huanwu Decoction (补阳还五汤) Attenuates Glial Scar by Downregulating Expression of Leukemia Inhibitory Factor in Intracerebral Hemorrhagic Rats

Xiao KANG; Hua-jun ZHOU; Jian YANG; Jian-hua ZHONG; Tao TANG; Han-jin CUI; Jing-hua ZHOU; Zhi-gang MEI

doi:10.1007/s11655-018-2917-7

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Buyang Huanwu Decoction (补阳还五汤) Attenuates Glial Scar by Downregulating Expression of Leukemia Inhibitory Factor in Intracerebral Hemorrhagic Rats

Original Article | Updated：2021-08-27

- Buyang Huanwu Decoction (补阳还五汤) Attenuates Glial Scar by Downregulating Expression of Leukemia Inhibitory Factor in Intracerebral Hemorrhagic Rats
- Buyang Huanwu Decoction (补阳还五汤) Attenuates Glial Scar by Downregulating Expression of Leukemia Inhibitory Factor in Intracerebral Hemorrhagic Rats
- Chinese Journal of Integrative Medicine 2019年25卷第4期页码：264-269
- Affiliations：
  
  1.Institute of Neurology, China Three Gorges University, Yichang, Hubei Province (443003), China
  2.Department of Neurology, The First College of Clinical Medical Sciences, China Three Gorges University, Yichang, Hubei Province (443003), China
  3.Key Laboratory of Cardiovascular and Cerebrovascular Diseases Translational Medicine, China Three Gorges University, Yichang, Hubei Province (443003), China
  4.Department of Intensive Care Unit, The First College of Clinical Medical Sciences, China Three Gorges University, Yichang, Hubei Province (443003), China
  5.Institute of Integrative Medicine, Xiangya Hospital, Central South University; Changsha (410008), China
  6.Medical College of China Three Gorges University, Yichang, Hubei Province (443002), China
- Author bio：
  
  Correspondence to: Dr. ZHOU Hua-jun, Tel: 86-717-6482069, E-mail: zhouhuajun02@126.com.
- Funds：
  
  the National Natural Science Foundation of China(81202625;30400581;30873221;81173175);the Project for New Century Excellent Talents(NCET-11-0522);the Hunan Provincial Natural Science Foundation(07JJ5007;10JJ2023);the Hubei Provincial Natural Science Foundation(2017CFB468);the Key Laboratory of Cardiovascular and Cerebrovascular Diseases Translational Medicine (Three Gorges University)(2016KXN06)
- DOI：10.1007/s11655-018-2917-7
  中图分类号：
- 纸质出版日期：2019-04-01，
  
  网络出版日期：2018-12-28，
  
  录用日期：2018-04-02
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Xiao KANG, Hua-jun ZHOU, Jian YANG, 等. Buyang Huanwu Decoction (补阳还五汤) Attenuates Glial Scar by Downregulating Expression of Leukemia Inhibitory Factor in Intracerebral Hemorrhagic Rats[J]. Chinese Journal of Integrative Medicine, 2019,25(4):264-269.

Xiao KANG, Hua-jun ZHOU, Jian YANG, et al. Buyang Huanwu Decoction (补阳还五汤) Attenuates Glial Scar by Downregulating Expression of Leukemia Inhibitory Factor in Intracerebral Hemorrhagic Rats[J]. Chinese Journal of Integrative Medicine, 2019,25(4):264-269.
Xiao KANG, Hua-jun ZHOU, Jian YANG, 等. Buyang Huanwu Decoction (补阳还五汤) Attenuates Glial Scar by Downregulating Expression of Leukemia Inhibitory Factor in Intracerebral Hemorrhagic Rats[J]. Chinese Journal of Integrative Medicine, 2019,25(4):264-269. DOI： 10.1007/s11655-018-2917-7.

Xiao KANG, Hua-jun ZHOU, Jian YANG, et al. Buyang Huanwu Decoction (补阳还五汤) Attenuates Glial Scar by Downregulating Expression of Leukemia Inhibitory Factor in Intracerebral Hemorrhagic Rats[J]. Chinese Journal of Integrative Medicine, 2019,25(4):264-269. DOI： 10.1007/s11655-018-2917-7.

摘要

Abstract

Objective:

To evaluate the effect of Buyang Huanwu Decoction (补阳还五汤

BYHWD) on glial scar after intracerebral hemorrhage (ICH) and investigate the underlying mechanism.

Methods:

Collagenase type Ⅶ (0.5 U) was injected stereotaxically into right globus pallidus to induce ICH model. One hundred and twenty Sprague-Dawley rats were randomly divided into 3 groups according to a random number table

including normal group (

=40)

ICH model group (

=40) and BYHWD group (

=40)

respectively. After ICH

the rats in the BYHWD group were intragastrically administered with BYHWD (4.36 g/kg) once a day for 21 days

while the rats in ICH group were administered with equal volume of distilled water for 21 days

respectively. Double immunolabeling was performed for proliferating cell nuclear antigen (PCNA)

/glial fibrillary acidic protein (GFAP)

nuclei. The expression of GFAP and leukemia inhibitory factor (LIF) was evaluated by immunohistochemistry and quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR).

Results:

The astrocytes with hypertrophied morphology around the hematoma was observed on day 3 after ICH. The number of GFAP positive cells and GFAP mRNA levels increased notably on day 3 and reached the peak on day 14 post-ICH (

0.01). PCNA

/GFAP

nuclei were observed around the hematoma and reached the peak on day 14 post-ICH (

0.01). In addition

LIF-positive astrocytes and LIF mRNA level in the hemorrhagic region increased significantly till day 14 post-ICH (

0.01). However

BYHWD not only reduced the number of PCNA

/GFAP

nuclei

but also decreased GFAP and LIF levels (

0.05).

Conclusion:

BYHWD could attenuate ICH-induced glial scar by downregulating the expression of LIF in the rats.

关键词

Keywords

Buyang Huanwu Decoctionintracerebral hemorrhageglial scarleukemia inhibitory factorChinese medicine

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