Acanthopanax senticosus Protects Structure and Function of Mesencephalic Mitochondria in A Mouse Model of Parkinson’s Disease

Shu-min Liu; Xu-zhao Li; Shuai-nan Zhang; Zhi-ming Yang; Ke-xin Wang; Fang Lu; Chong-zhi Wang; Chun-su Yuan

doi:10.1007/s11655-018-2935-5

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Acanthopanax senticosus Protects Structure and Function of Mesencephalic Mitochondria in A Mouse Model of Parkinson’s Disease

OriginalPaper | Updated：2021-08-27

- Acanthopanax senticosus Protects Structure and Function of Mesencephalic Mitochondria in A Mouse Model of Parkinson’s Disease
- Acanthopanax senticosus Protects Structure and Function of Mesencephalic Mitochondria in A Mouse Model of Parkinson’s Disease
- 中国结合医学杂志（英文版） 2018年24卷第11期页码：835-843
- Affiliations：
  
  1. Drug Safety Evaluation Center, Heilongjiang University of Chinese Medicine,Harbin,China
  2. Department of Pharmacy, Guiyang College of Traditional Chinese Medicine,Guiyang,China
  3. Institute of Traditional Chinese Medicine, Heilongjiang University of Chinese Medicine,Harbin,China
  4. Tang Center for Herbal Medicine Research, and Department of Anesthesia and Critical Care, University of Chicago,Chicago,USA
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81270056), China Postdoctoral Science Foundation (No. 2013T60398), Scientific Research grants of Postdoctoral Researchers Settled in Heilongjiang (No. LBH-Q13160), Outstanding Talents Cultivation Fund of Heilongjiang University of Chinese Medicine (No. 2013jc01), and the Outstanding Innovative Talent Support Programs of Heilongjiang University of Chinese Medicine
- DOI：10.1007/s11655-018-2935-5
  中图分类号：
- 纸质出版日期：2018，
  
  网络出版日期：2018-8-8，
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Liu, Sm., Li, Xz., Zhang, Sn. et al. Acanthopanax senticosus Protects Structure and Function of Mesencephalic Mitochondria in A Mouse Model of Parkinson’s Disease., Chin. J. Integr. Med. 24, 835–843 (2018). https://doi.org/10.1007/s11655-018-2935-5

Shu-min Liu, Xu-zhao Li, Shuai-nan Zhang, et al. Acanthopanax senticosus Protects Structure and Function of Mesencephalic Mitochondria in A Mouse Model of Parkinson’s Disease[J]. Chinese Journal of Integrative Medicine, 2018,24(11):835-843.
Liu, Sm., Li, Xz., Zhang, Sn. et al. Acanthopanax senticosus Protects Structure and Function of Mesencephalic Mitochondria in A Mouse Model of Parkinson’s Disease., Chin. J. Integr. Med. 24, 835–843 (2018). https://doi.org/10.1007/s11655-018-2935-5 DOI：

Shu-min Liu, Xu-zhao Li, Shuai-nan Zhang, et al. Acanthopanax senticosus Protects Structure and Function of Mesencephalic Mitochondria in A Mouse Model of Parkinson’s Disease[J]. Chinese Journal of Integrative Medicine, 2018,24(11):835-843. DOI： 10.1007/s11655-018-2935-5.

摘要

To investigate the neuro-protective effects of Acanthopanax senticosus Harms (EAS) on mesencephalic mitochondria and the mechanism of action

using a mouse model of Parkinson’s disease (PD). The chemical fingerprint analysis of the extract of Acanthopanax senticosus Harms (EAS) was performed using the ultra performance liquid chromatograph and time of flight mass spectrometry. Thirty mice were randomly divided into the control group

the MPTP model group

and the EAS treated group with MPTP (MPTP+EAS group

10 in each group). The MPTP model group and the MPTP+EAS group received MPTP-HCl (30 mg/kg i.p) once a day for 5 days. The control group received an equal volume of saline (20 mL/kg i.p) once a day for 5 days. Induced by 1-methyl-4-phenyl-1

6-tetrahydropyridine hydrochloride daily (MPTP-HCl

30 mg/kg) for 5 days

the PD mice were treated with EAS at 45.5 mg/kg daily for 20 days. The behavioral testing of mice was carried out using the pole-climbing test. The integrity and functions of neurons were examined in mesencephalic mitochondria in a PD mouse model

including nicotinamide adenine dinucleotide dehydrogenase ubiquinone flavoprotein 2 (NDUFV2)

mitochondrially encoded nicotinamide adenine dinucleotide dehydrogenase 1 (MT-ND1)

succinate dehydrogenase complex subunit A (SDHA)

and succinate dehydrogenase cytochrome b560 subunit (SDHC). After treatment with EAS

the behavioral changes induced by MPTP were attenuated significantly (P<0.05). EAS protected the mesencephalic mitochondria from swelling and attenuated the decreases in their membrane potential (both P<0.05)

which was supported by an ultra-structural level analysis. The changes in reactive oxygen species (ROS)

malonic dialdehyde (MDA)

oxidative phosphorylation (OXPHOS) system 4 subunits levels and PD-related proteins expressions (parkin

Pink1

DJ-1

α-synuclein

and Lrrk2) reverted to near normal levels (all P<0.05)

based on the results of immune-histological and Western blotting observations. The neuro-protective effects of EAS are linked to protecting mice against MPTP-induced mitochondrial dysfunction and structural damage. Therefore

EAS is a promising candidate for the prevention or treatment of mitochondrial neurodegenerative disorders

such as PD.

Abstract

To investigate the neuro-protective effects of Acanthopanax senticosus Harms (EAS) on mesencephalic mitochondria and the mechanism of action

the MPTP model group

and the EAS treated group with MPTP (MPTP+EAS group

6-tetrahydropyridine hydrochloride daily (MPTP-HCl

30 mg/kg) for 5 days

including nicotinamide adenine dinucleotide dehydrogenase ubiquinone flavoprotein 2 (NDUFV2)

mitochondrially encoded nicotinamide adenine dinucleotide dehydrogenase 1 (MT-ND1)

succinate dehydrogenase complex subunit A (SDHA)

and succinate dehydrogenase cytochrome b560 subunit (SDHC). After treatment with EAS

which was supported by an ultra-structural level analysis. The changes in reactive oxygen species (ROS)

malonic dialdehyde (MDA)

oxidative phosphorylation (OXPHOS) system 4 subunits levels and PD-related proteins expressions (parkin

Pink1

DJ-1

α-synuclein

and Lrrk2) reverted to near normal levels (all P<0.05)

EAS is a promising candidate for the prevention or treatment of mitochondrial neurodegenerative disorders

such as PD.

关键词

Acanthopanax senticosus HarmsParkinson’s diseasemitochondrial dysfunctionmitochondrial structural damageoxidative phosphorylation system

Keywords

Acanthopanax senticosus HarmsParkinson’s diseasemitochondrial dysfunctionmitochondrial structural damageoxidative phosphorylation system

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