Pien Tze Huang (片仔癀) Overcomes Doxorubicin Resistance and Inhibits Epithelial-Mesenchymal Transition in MCF-7/ADR Cells

Xi CHEN; Fei QI; A-ling SHEN; Jian-feng CHU; Thomas Joseph Sferra; You-qin CHEN; Jun PENG

doi:10.1007/s11655-018-2992-4

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Pien Tze Huang (片仔癀) Overcomes Doxorubicin Resistance and Inhibits Epithelial-Mesenchymal Transition in MCF-7/ADR Cells

Original Article | Updated：2021-08-27

- Pien Tze Huang (片仔癀) Overcomes Doxorubicin Resistance and Inhibits Epithelial-Mesenchymal Transition in MCF-7/ADR Cells
- Pien Tze Huang (片仔癀) Overcomes Doxorubicin Resistance and Inhibits Epithelial-Mesenchymal Transition in MCF-7/ADR Cells
- Chinese Journal of Integrative Medicine 2019年25卷第8期页码：598-603
- Affiliations：
  
  1.Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou (350122), China
  2.Department of Oncology, Fuzhou General Hospital of Nanjing Military Command, Fuzhou (350003), China
  3.Fujian Key Laboratory of Integrative Medicine on Geriatric, Fujian University of Traditional Chinese Medicine, Fuzhou (350122), China
  4.School of Medicine, Case Western Reserve University, Cleveland, OH (44106), USA
- Author bio：
  
  Correspondence to: Dr. PENG Jun, Tel: 86-591-22861303, E-mail: pjunlab@hotmail.com
- Funds：
  
  the National Natural Science Foundation of China(81673721);Natural Science Foundation of Fujian Province(2018j01352);the Developmental Fund of Chen Keji Integrative Medicine(CKJ2014004)
- DOI：10.1007/s11655-018-2992-4
  中图分类号：
- 纸质出版日期：2019-08-01，
  
  网络出版日期：2018-08-22，
  
  录用日期：2017-07-14
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Xi CHEN, Fei QI, A-ling SHEN, 等. Pien Tze Huang (片仔癀) Overcomes Doxorubicin Resistance and Inhibits Epithelial-Mesenchymal Transition in MCF-7/ADR Cells[J]. Chinese Journal of Integrative Medicine, 2019,25(8):598-603.

Xi CHEN, Fei QI, A-ling SHEN, et al. Pien Tze Huang (片仔癀) Overcomes Doxorubicin Resistance and Inhibits Epithelial-Mesenchymal Transition in MCF-7/ADR Cells[J]. Chinese Journal of Integrative Medicine, 2019,25(8):598-603.
Xi CHEN, Fei QI, A-ling SHEN, 等. Pien Tze Huang (片仔癀) Overcomes Doxorubicin Resistance and Inhibits Epithelial-Mesenchymal Transition in MCF-7/ADR Cells[J]. Chinese Journal of Integrative Medicine, 2019,25(8):598-603. DOI： 10.1007/s11655-018-2992-4.

Xi CHEN, Fei QI, A-ling SHEN, et al. Pien Tze Huang (片仔癀) Overcomes Doxorubicin Resistance and Inhibits Epithelial-Mesenchymal Transition in MCF-7/ADR Cells[J]. Chinese Journal of Integrative Medicine, 2019,25(8):598-603. DOI： 10.1007/s11655-018-2992-4.

摘要

Abstract

Objective:

To evaluate the effect of Pien Tze Huang (片仔癀

PZH) on breast cancer chemoresistance and related epithelial-mesenchymal transition (EMT) and investigate the underlying mechanisms.

Methods:

3-(4

5-Dimethyl-2-thiazolyl)-2

5-diphenyl-2-H-tetrazolium bromide (MTT) assay was used to determine the cell viability. Adriamycin (ADR) staining observed by fluorescence microscope was performed to detect the accumulation of ADR. Transwell assay was used to analyze the cell migration and invasion. Western-blot was performed to detect the protein expression of related genes.

Results:

MCF-7/ADR cells were resistant to ADR treatment

and PZH treatment inhibited the viability of MCF-7/ADR cells in a dose-dependent manner. PZH treatment also increased the intercellular accumulation of ADR and down-regulated the expression of ABCG2 and ABCB1 in MCF-7/ADR cells (

0.05). In addition

PZH treatment inhibited EMT

migration and invasion of MCF-7/ADR cells (

0.05). Moreover

PZH suppressed activation of transforming growth factor β1 (TGF-β) signaling in MCF-7/ADR cells (

0.05).

Conclusion:

PZH treatment can effectively overcome chemoresistance via down-regulating ABCG2

ABCB1 and inhibit EMT in ADR resistant human breast cancer cells via suppression of the TGF-β1 pathway.

关键词

Keywords

Pien Tze HuangChinese medicinebreast cancerchemoresistanceepithelial-mesenchymal transition

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