San-Ao Decoction (三拗汤) Regulates Urine Volume on Bronchial Asthma Model Mice

Min WANG; Yan SUN; Shu-jing ZHANG; Yu-shan GAO; Ya-nan WEI; Peng-na ZHAO; Miao LIU; Jia-min YANG; Feng-jie ZHENG; Hong XU; Yu-hang LI

doi:10.1007/s11655-018-3006-2

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San-Ao Decoction (三拗汤) Regulates Urine Volume on Bronchial Asthma Model Mice

Original Article | Updated：2021-08-16

- San-Ao Decoction (三拗汤) Regulates Urine Volume on Bronchial Asthma Model Mice
- San-Ao Decoction (三拗汤) Regulates Urine Volume on Bronchial Asthma Model Mice
- Chinese Journal of Integrative Medicine 2021年27卷第3期页码：212-219
- Affiliations：
  
  School of Chinese Medicine, Beijing University of Chinese Medicine, Beijing (100029), China
- Author bio：
  
  Correspondence to: Prof. LI Yu-hang, E-mail: liyuhang@bucm.edu.cn
- Funds：
  
  the National Natural Science Foundation
- DOI：10.1007/s11655-018-3006-2
  中图分类号：
- 纸质出版日期：2021-03-01，
  
  网络出版日期：2018-10-17，
  
  录用日期：2018-01-19
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Min WANG, Yan SUN, Shu-jing ZHANG, 等. San-Ao Decoction (三拗汤) Regulates Urine Volume on Bronchial Asthma Model Mice[J]. Chinese Journal of Integrative Medicine, 2021,27(3):212-219.

Min WANG, Yan SUN, Shu-jing ZHANG, et al. San-Ao Decoction (三拗汤) Regulates Urine Volume on Bronchial Asthma Model Mice[J]. Chinese Journal of Integrative Medicine, 2021,27(3):212-219.
Min WANG, Yan SUN, Shu-jing ZHANG, 等. San-Ao Decoction (三拗汤) Regulates Urine Volume on Bronchial Asthma Model Mice[J]. Chinese Journal of Integrative Medicine, 2021,27(3):212-219. DOI： 10.1007/s11655-018-3006-2.

Min WANG, Yan SUN, Shu-jing ZHANG, et al. San-Ao Decoction (三拗汤) Regulates Urine Volume on Bronchial Asthma Model Mice[J]. Chinese Journal of Integrative Medicine, 2021,27(3):212-219. DOI： 10.1007/s11655-018-3006-2.

摘要

Abstract

Objective:

To observe the effect of San-Ao Decoction (三拗汤

SAD) on water metabolism of bronchial asthra model mice.

Methods:

Forty-five female BALB/c mice were randomly divided into control

model and SAD groups by a random number table

15 mice in each group. A composite method with ovalbumin (OVA) sensitization and challenge was developed to establish bronchial asthma model. Mice in the control group were intraperitoneally injected with distilled water without aerosol inhalation challenge. On day 15–22

0.3 mL SAD was administered via gastric route in SAD group

one time per day

while an equivalent volume of normal saline was used for gastric administration in the control and model groups. Changes in airway resistance in the inspiratory phase (RI-R-Area) were detected using an AniRes2005 system

and 5-h urine output was collected by metabolic cages. Histopathological changes in lung and kidney were observed by hematoxylin-eosin staining. mRNA expressions of aquaporin (AQP) 1 and AQP2 in kidney were detected by reverse transcription-polymerase chain reaction

and the protein expressions of AQP1 and AQP2 in kidney were detected by immunohistochemistry. Enzyme-linked immune sorbent assay was used to detect the OVA-specific endothelium-1 (ET-1)

antidiuretic hormone (ADH)

atrial natriuretic peptide (ANP)

prostaglandin E

(PGE

)

and angiotensin Ⅱ (Ang Ⅱ) levels in serum

lung and kidney tissues

respectively. The nitric oxide (NO) contents in serum

lung

and kidney tissues were tested by chemical method

respectively.

Results:

Compared with the control group

the serum IgE level in model group increased (

0.01). Following the pathologic changes in lung tissue

no significant change in kidney tissue was observed among 3 groups. Compared with the control group

the mice in the model group showed elevated airway resistance during inhalation phase

higher mRNA and protein expression levels on AQP1 and AQP2 in kidney tissue and higher ET-1 levels in serum

lung and kidney tissues

ADH and ANP in lung and serum

PGE

in kidney

Ang Ⅱ in lung and kidney tissues (

0.05 or

0.01)

but decreased in 5-h urinary output as well as NO and PGE

contents in serum and lung tissues (

0.05 or

0.01). Compared with the model group

the mice in the SAD group showed a weakened airway resistance in inspiratory phase

lower mRNA and protein expressions of AQP1 and AQP2 in kidney tissues

lower levels of ET-1

ADH

ANP in serum as well as ET-1

ANP

Ang Ⅱ levels in kidney tissues (

0.05 or

0.01)

whereas 5-h urinary output

NO content in kidney

ADH

ANP and PGE

levels in lung and Ang Ⅱ in serum increased (

0.05 or

0.01).

Conclusion:

San-Ao Decoction can regulate the urine volume through regulating AQP1 and AQP2 expression

and the expression of these in the kidneys might be regulated by ET-1

NO and Ang Ⅱ.

关键词

Keywords

regulation of fluid passageurine volumeaquaporinSan-Ao Decoctionbronchial asthma

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