Acupuncture Regulates the Balance of CD4<sup>+</sup> T Cell Subtypes in Experimental Asthma Mice

Ming DONG; Wen-qian WANG; Juan CHEN; Mi-hui LI; Fei XU; Jie CUI; Jing-cheng DONG; Ying WEI

doi:10.1007/s11655-018-3055-6

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Acupuncture Regulates the Balance of CD4⁺ T Cell Subtypes in Experimental Asthma Mice

Acupuncture Research | Updated：2021-08-27

- Acupuncture Regulates the Balance of CD4⁺ T Cell Subtypes in Experimental Asthma Mice
- Acupuncture Regulates the Balance of CD4⁺ T Cell Subtypes in Experimental Asthma Mice
- Chinese Journal of Integrative Medicine 2019年25卷第8期页码：617-624
- Affiliations：
  
  1.Gumei Community Health Center of Minhang District, Shanghai (201102), China
  2.Department of Integrative Medicine, Huashan Hospital, Fudan University, Shanghai (200040), China
  3.Institutes of Integrative Medicine of Fudan University, Shanghai (200040), China
  4.Department of Pediatric Neurological Rehabilitation, Maternal and Child Health Hospital of Dengfeng, Dengfeng (452470), Henan Province , China
- Author bio：
  
  Correspondence to: Associate Prof. WEI Ying, Tel: 86-21-52888263, E-mail: weiying_acup@126.com
- Funds：
  
  the National Natural Science Foundation of China(81403476)
- DOI：10.1007/s11655-018-3055-6
  中图分类号：
- 纸质出版日期：2019-08-01，
  
  网络出版日期：2018-12-05，
  
  录用日期：2017-06-26
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Ming DONG, Wen-qian WANG, Juan CHEN, 等. Acupuncture Regulates the Balance of CD4⁺ T Cell Subtypes in Experimental Asthma Mice[J]. Chinese Journal of Integrative Medicine, 2019,25(8):617-624.

Ming DONG, Wen-qian WANG, Juan CHEN, et al. Acupuncture Regulates the Balance of CD4⁺ T Cell Subtypes in Experimental Asthma Mice[J]. Chinese Journal of Integrative Medicine, 2019,25(8):617-624.
Ming DONG, Wen-qian WANG, Juan CHEN, 等. Acupuncture Regulates the Balance of CD4⁺ T Cell Subtypes in Experimental Asthma Mice[J]. Chinese Journal of Integrative Medicine, 2019,25(8):617-624. DOI： 10.1007/s11655-018-3055-6.

Ming DONG, Wen-qian WANG, Juan CHEN, et al. Acupuncture Regulates the Balance of CD4⁺ T Cell Subtypes in Experimental Asthma Mice[J]. Chinese Journal of Integrative Medicine, 2019,25(8):617-624. DOI： 10.1007/s11655-018-3055-6.

摘要

Abstract

Objective:

To evaluate the involvement of different CD4

T cell subtypes in the anti-asthmatic effects of acupuncture in asthmatic mice.

Methods:

BALB/c mice were challenged by ovalbumin (OVA) for the establishment of experimental asthma model. Mice were divided into 4 groups by a random number table including the normal control

asthma model

acupuncture and sham acupuncture groups (14 per group). Acupoints Dazhui (GV 14)

bilateral Fengmen (BL 12) and Feishu (BL 13) were selected for manual acupuncture treatment every other day for 4 weeks and Huantiao (GB 30) was selected for sham acupuncture. Airway hyperresponsiveness was examined by Buxco Pulmonary System. Pulmonary histopathology analysis was performed for inflammatory cell infiltration and mucus hypersecretion by haematoxylin eosin staining and periodic acid-Schiff staining. Inflammatory mediators assays of serum were investigated by enzyme-linked immunosorbent assay and Bio-Plex. CD4

T cell subpopulations including the expression levels of important factors in T lymphocyte polarization in lung tissue were examined by flow cytometric and Western blot analyses. Related pathways were detected by Western blot assay.

Results:

Compared with the OVA-induced asthma model group

acupuncture could attenuate airway hyperresponsiveness

inhibit inflammatory cell infiltration and mucus hypersecretion (

0.05 or

0.01). Furthermore

acupuncture increased the expressions of T-bet and Foxp3

the cell numbers of CD4

interferon gamma (IFN-γ)

and CD4

Foxp3

in lung tissue and the level of Treg type cytokine interleukin (IL)-10 in serum (

0.05 or

0.01). Meanwhile

acupuncture reduced the RAR-related orphan receptor gamma t (RORγt) level

the cell numbers of CD4

IL-17A

as well as the levels of IL-5

IL-13 and IL-17A in serum (

0.05 or

0.01). In addition

both acupuncture and sham acupuncture could inhibit the phosphorylation of p38 and p44/42 (

0.01).

Conclusion:

Acupuncture could alleviate allergic airway inflammation by strengthening the activities of Th1 and Treg

thus regulating the balance of CD4

T cell subtypes in experimental asthmatic mice.

关键词

Keywords

acupunctureasthmaTh2Th17Treg

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Acupuncture Regulates the Balance of CD4+ T Cell Subtypes in Experimental Asthma Mice

Acupuncture Regulates the Balance of CD4+ T Cell Subtypes in Experimental Asthma Mice

DOI：10.1007/s11655-018-3055-6

摘要

Abstract

关键词

Keywords

references

Acupuncture Regulates the Balance of CD4⁺ T Cell Subtypes in Experimental Asthma Mice

Acupuncture Regulates the Balance of CD4⁺ T Cell Subtypes in Experimental Asthma Mice