Low-Frequency Electroacupuncture Alleviates Chronic Constrictive Injury-Induced Mechanical Allodynia by Inhibiting NR2B Upregulation in Ipsilateral Spinal Dorsal Horn in Rats

Wen-sheng ZHAO; Zhen-ni JIANG; Hui SHI; Lu-lu XU; Yue YANG; Ying-chao WANG

doi:10.1007/s11655-018-3057-4

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Low-Frequency Electroacupuncture Alleviates Chronic Constrictive Injury-Induced Mechanical Allodynia by Inhibiting NR2B Upregulation in Ipsilateral Spinal Dorsal Horn in Rats

Acupuncture Research | Updated：2021-08-27

- Low-Frequency Electroacupuncture Alleviates Chronic Constrictive Injury-Induced Mechanical Allodynia by Inhibiting NR2B Upregulation in Ipsilateral Spinal Dorsal Horn in Rats
- Low-Frequency Electroacupuncture Alleviates Chronic Constrictive Injury-Induced Mechanical Allodynia by Inhibiting NR2B Upregulation in Ipsilateral Spinal Dorsal Horn in Rats
- Chinese Journal of Integrative Medicine 2019年25卷第6期页码：462-467
- Affiliations：
  
  1.Department of Pain Medicine, Hangzhou Red Cross Hospital, Hangzhou (310003), China
  2.Department of Cardiology, The Second Affiliated Hospital of Zhejiang University, School of Medicine, Hangzhou (310009), China
  3.Department of Acupuncture, The Second Affiliated Hospital of Zhejiang University, School of Medicine, Hangzhou (310009), China
  4.The Second Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou (310053), China
  5.Department of Clinical Research Center, The Second Affiliated Hospital of Zhejiang University, School of Medicine, Hangzhou (310009), China
- Author bio：
  
  Correspondence to: Dr. JIANG Zhen-ni, Tel: 86-571-87784709, Fax: 86-571-87068001, E-mail: ze_jzn@zju.edu.cn
- Funds：
  
  the Zhejiang Provincial Natural Science Foundation of China(LY14H270007)
- DOI：10.1007/s11655-018-3057-4
  中图分类号：
- 纸质出版日期：2019-06-01，
  
  网络出版日期：2018-11-22，
  
  录用日期：2018-06-08
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Wen-sheng ZHAO, Zhen-ni JIANG, Hui SHI, 等. Low-Frequency Electroacupuncture Alleviates Chronic Constrictive Injury-Induced Mechanical Allodynia by Inhibiting NR2B Upregulation in Ipsilateral Spinal Dorsal Horn in Rats[J]. Chinese Journal of Integrative Medicine, 2019,25(6):462-467.

Wen-sheng ZHAO, Zhen-ni JIANG, Hui SHI, et al. Low-Frequency Electroacupuncture Alleviates Chronic Constrictive Injury-Induced Mechanical Allodynia by Inhibiting NR2B Upregulation in Ipsilateral Spinal Dorsal Horn in Rats[J]. Chinese Journal of Integrative Medicine, 2019,25(6):462-467.
Wen-sheng ZHAO, Zhen-ni JIANG, Hui SHI, 等. Low-Frequency Electroacupuncture Alleviates Chronic Constrictive Injury-Induced Mechanical Allodynia by Inhibiting NR2B Upregulation in Ipsilateral Spinal Dorsal Horn in Rats[J]. Chinese Journal of Integrative Medicine, 2019,25(6):462-467. DOI： 10.1007/s11655-018-3057-4.

Wen-sheng ZHAO, Zhen-ni JIANG, Hui SHI, et al. Low-Frequency Electroacupuncture Alleviates Chronic Constrictive Injury-Induced Mechanical Allodynia by Inhibiting NR2B Upregulation in Ipsilateral Spinal Dorsal Horn in Rats[J]. Chinese Journal of Integrative Medicine, 2019,25(6):462-467. DOI： 10.1007/s11655-018-3057-4.

摘要

Abstract

Objective:

To study the effects of electroacupuncture (EA) in chronic constrictive injury (CCI) rat model and the expression of N-methyl-D-aspartate receptor type 2B (NR2B) in ipsilateral spinal dorsal horn in rats to explore the analgesic mechanisms of EA.

Methods:

According to the random number table

totally 180 rats were evenly divided into a sham group

a CCI group

and an EA group. CCI model was conducted with four 4–0 chromic gut ligatures loosely ligated around the left sciatic nerve 1 cm above the trifurcation. Rats in the EA group received 2 Hz EA therapy bilaterally at acupoints of Zusanli (ST 36) and Sanyinjiao (SP 6) once daily (30 min/d) for 30 days after surgery. Paw withdrawal thresholds (PWTs) were measured on 0 (baseline)

30 days after surgery. Rats were sacrificed on 0

15 and 30 days after surgery

and the L4–5 segments of spinal cord were removed to detect the expression of NR2B by immunohistochemistry and quantitative polymerase chain reaction.

Results:

PWTs in the CCI group were significantly lower than the sham group at Day 1–30 after surgery

and reached its lowest at Day 1 (

0.01). After EA treatment

the PWTs recovered rapidly and were significantly higher than those in the CCI group on 3

15 and 30 days after surgery (

0.01). The numbers of NR2B-immunoreactive cells of the CCI group significantly increased after CCI surgery compared with the sham group (

0.01). Compared with the CCI group

stimulation of EA markedly decreased the numbers of NR2B-immunoreactive cells at Day 3

15 and 30 (

0.05). In the sham group

NR2B mRNA was expressed at a low level. It increased after CCI surgery

which increased rapidly at Day 7 (

0.01) and reached its peak value at Day 15 (

0.01). After EA stimulation

relative quantity of NR2B mRNA expression was less than that in the CCI group at Day 15 and 30 (

0.05).

Conclusions:

Low frequency of EA had antinociceptive effect in CCI rat model. The analgesic effects of EA might be through the inhibition of NR2B.

关键词

Keywords

sciaticaelectroacupuncturechronic constrictive injuryspinal dorsal hornN-methyl-D-aspartate receptor type 2BChinese medicine

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