Qingxuan Jiangya Decoction (清眩降压汤) Prevents Blood Pressure Elevation and Ameliorates Vascular Structural Remodeling via Modulating TGF-β1/Smad Pathway in Spontaneously Hypertensive Rats

Fei HE; Jian-feng CHU; Hong-wei CHEN; Wei LIN; Shan LIN; You-qin CHEN; Jun PENG; Ke-ji CHEN

doi:10.1007/s11655-019-2705-7

Your Location：

Home >

Browse articles >

Qingxuan Jiangya Decoction (清眩降压汤) Prevents Blood Pressure Elevation and Ameliorates Vascular Structural Remodeling via Modulating TGF-β1/Smad Pathway in Spontaneously Hypertensive Rats

Original Article | Updated：2021-08-23

- Qingxuan Jiangya Decoction (清眩降压汤) Prevents Blood Pressure Elevation and Ameliorates Vascular Structural Remodeling via Modulating TGF-β1/Smad Pathway in Spontaneously Hypertensive Rats
- Qingxuan Jiangya Decoction (清眩降压汤) Prevents Blood Pressure Elevation and Ameliorates Vascular Structural Remodeling via Modulating TGF-β1/Smad Pathway in Spontaneously Hypertensive Rats
- Chinese Journal of Integrative Medicine 2020年26卷第3期页码：180-187
- Affiliations：
  
  1.Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou (350122), China
  2.Chen Keji Academic Thought Inheritance Studio, Fujian University of Traditional Chinese Medicine, Fuzhou (350122), China
  3.Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fuzhou (350122), China
  4.Case Western Reserve University School of Medicine, Rainbow Babies and Children's Hospital, Cleveland, OH 44106, USA
  5.Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing (100091), China
- Author bio：
  
  Correspondence to: Dr. PENG Jun, E-mail:pjunlab@hotmail.com and
  Dr. CHEN Ke-ji, E-mail:kjchenvip@163.com
- Funds：
  
  the National Natural Science Foundation of China(81774135);the Developmental Fund of Chen Keji Integrative Medicine(CKJ2016004;CKJ2017001)
- DOI：10.1007/s11655-019-2705-7
  中图分类号：
- 纸质出版日期：2020-03-01，
  
  网络出版日期：2019-12-27，
  
  录用日期：2017-11-15
Scan for full text
Fei HE, Jian-feng CHU, Hong-wei CHEN, 等. Qingxuan Jiangya Decoction (清眩降压汤) Prevents Blood Pressure Elevation and Ameliorates Vascular Structural Remodeling via Modulating TGF-β1/Smad Pathway in Spontaneously Hypertensive Rats[J]. Chinese Journal of Integrative Medicine, 2020,26(3):180-187.

Fei HE, Jian-feng CHU, Hong-wei CHEN, et al. Qingxuan Jiangya Decoction (清眩降压汤) Prevents Blood Pressure Elevation and Ameliorates Vascular Structural Remodeling via Modulating TGF-β1/Smad Pathway in Spontaneously Hypertensive Rats[J]. Chinese Journal of Integrative Medicine, 2020,26(3):180-187.
Fei HE, Jian-feng CHU, Hong-wei CHEN, 等. Qingxuan Jiangya Decoction (清眩降压汤) Prevents Blood Pressure Elevation and Ameliorates Vascular Structural Remodeling via Modulating TGF-β1/Smad Pathway in Spontaneously Hypertensive Rats[J]. Chinese Journal of Integrative Medicine, 2020,26(3):180-187. DOI： 10.1007/s11655-019-2705-7.

Fei HE, Jian-feng CHU, Hong-wei CHEN, et al. Qingxuan Jiangya Decoction (清眩降压汤) Prevents Blood Pressure Elevation and Ameliorates Vascular Structural Remodeling via Modulating TGF-β1/Smad Pathway in Spontaneously Hypertensive Rats[J]. Chinese Journal of Integrative Medicine, 2020,26(3):180-187. DOI： 10.1007/s11655-019-2705-7.

摘要

Abstract

Objective:

To elevate the effects of Qingxuan Jiangya Decoction (清眩降压汤

QXJYD) on hypertension and vascular structural remodeling (VSR) in spontaneously hypertensive rats (SHRs)

and investigate the underlying mechanisms.

Methods:

SHRs (

=8) were given intra-gastric administration with 60 mg/kg of QXJYD or saline

daily for 8 weeks

while rats in SHR-control (

=8) and WKY (

=8) groups were received equal volumes of saline solution. Systolic blood pressures (SBP)

diastolic blood pressures (DBP) and mean blood pressures (MBP) were measured once a week. The levels of angiotensin Ⅱ (Ang Ⅱ)

endothelin 1 (ET-1) and plasma renin activity (PRA) were tested by enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay

respectively. The effect of QXJYD on VSR was determined by examining the media thickness and the ex vivo contractility of thoracic aortic. The proliferation and fibrosis of vascular smooth muscle cells (VSMCs) were examined via immunohistochemical (IHC) staining for proliferating cell nuclear antigen (PCNA)

collagen Ⅰ and collagen Ⅲ

respectively. The mRNA and protein expressions of transforming growth factorβ1 (TGF-β1)

Smad3 and phosphorylation of Smad3 in thoracic aorta tissues were determined by real-time polymerase chain reaction (PCR) and Western blot assay

respectively.

Results:

QXJYD treatment led to a significant decrease of the elevation of blood pressure in SHRs and reduced the levels of Ang Ⅱ

ET-1 and PRA in the serum (

0.05). In addition

QXJYD treatment remarkably ameliorated VSR and vascular function in SHRs. Moreover

QXJYD inhibited VSMC proliferation and fibrosis by suppressing the expression of PCNA

collagen Ⅰ and collagen Ⅲ in thoracic aortic. Furthermore

QXJYD inhibited the expression of TGF-β1

Smad3 and the phosphorylation of Smad3

respectively (

0.05).

Conclusion:

QXJYD reversed VSR by inhibiting VSMC proliferation and collagen deposition via regulation of TGF-β1/Smad signaling pathway

which may

in part

illuminate its anti-hypertensive activities.

关键词

Keywords

Qingxuan Jiangya DecoctionChinese medicinevascular structural remodelingtransforming growth factor-beta 1/Smad signaling pathway

references

More>

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Linggui Zhugan Decoction (苓桂术甘汤) Inhibits Ventricular Remodeling after Acute Myocardial Infarction in Rats by Suppressing TGF-β₁/Smad Signaling Pathway

Chemical Composition, Pharmacological Effects and Clinical Applications of Cinobufacini

Immunomodulatory Function of Pien Tze Huang in T Cell-Mediated Anti-tumor Activity against B16-F10, MC38 and Hep1-6 Tumor Models

Safflower Yellow Inhibits Progression of Hepatocellular Carcinoma by Modulating Immunological Tolerance via FAK Pathway

Immunoregulatory and Anti-cancer Activities of Combination Treatment of Novel Four-Herb Formula and Doxorubicin in 4T1-Breast Cancer Bearing Mice