Bear Bile Powder Inhibits Growth of Hepatocellular Carcinoma via Suppressing STAT3 Signaling Pathway in Mice

Hong-wei CHEN; A-ling SHEN; Li-ya LIU; Jun PENG; Jian-feng CHU

doi:10.1007/s11655-019-3010-1

Your Location：

Home >

Browse articles >

Bear Bile Powder Inhibits Growth of Hepatocellular Carcinoma via Suppressing STAT3 Signaling Pathway in Mice

Original Article | Updated：2021-08-23

- Bear Bile Powder Inhibits Growth of Hepatocellular Carcinoma via Suppressing STAT3 Signaling Pathway in Mice
- Bear Bile Powder Inhibits Growth of Hepatocellular Carcinoma via Suppressing STAT3 Signaling Pathway in Mice
- Chinese Journal of Integrative Medicine 2020年26卷第5期页码：370-374
- Affiliations：
  
  1.Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou (350122), China
  2.Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou (350122), China
- Author bio：
  
  Correspondence to: Dr. CHU Jian-feng, E-mail:jianfengchu@126.com
- Funds：
  
  the Developmental Fund of CHEN Ke-ji Integrative Medicine(CKJ2013009)
- DOI：10.1007/s11655-019-3010-1
  中图分类号：
- 纸质出版日期：2020-05-01，
  
  网络出版日期：2019-02-27，
  
  录用日期：2017-06-20
Scan for full text
Hong-wei CHEN, A-ling SHEN, Li-ya LIU, 等. Bear Bile Powder Inhibits Growth of Hepatocellular Carcinoma via Suppressing STAT3 Signaling Pathway in Mice[J]. Chinese Journal of Integrative Medicine, 2020,26(5):370-374.

Hong-wei CHEN, A-ling SHEN, Li-ya LIU, et al. Bear Bile Powder Inhibits Growth of Hepatocellular Carcinoma via Suppressing STAT3 Signaling Pathway in Mice[J]. Chinese Journal of Integrative Medicine, 2020,26(5):370-374.
Hong-wei CHEN, A-ling SHEN, Li-ya LIU, 等. Bear Bile Powder Inhibits Growth of Hepatocellular Carcinoma via Suppressing STAT3 Signaling Pathway in Mice[J]. Chinese Journal of Integrative Medicine, 2020,26(5):370-374. DOI： 10.1007/s11655-019-3010-1.

Hong-wei CHEN, A-ling SHEN, Li-ya LIU, et al. Bear Bile Powder Inhibits Growth of Hepatocellular Carcinoma via Suppressing STAT3 Signaling Pathway in Mice[J]. Chinese Journal of Integrative Medicine, 2020,26(5):370-374. DOI： 10.1007/s11655-019-3010-1.

摘要

Abstract

Objective:

To evaluate the inhibitory effect of bear bile powder (BBP) on hepatocellular carcinoma (HCC) growth

in vivo

and investigate the underlying mechanisms.

Methods:

A HCC xenograft mouse model was developed by producing with huh7 cells. After 5 days following xenograft implantation

ten HCC xenograft mice were given intra-gastric administration with 10 mg/(kg•d) dose of BBP or saline for 3 weeks. Tumor growth in HCC xenograft mice was evaluated by measuring the tumor weight and volume. Cell apoptosis

proliferation or tumor angiogenesis were examined via immunohistochemical (IHC) staining for transferase-mediated deoxyuridine triphosphate-biotin nick end labeling (TUNEL)

proliferating cell nuclear antigen (PCNA) or cluster of differentiation 31 (CD31)

respectively. Phosphorylation of signal transducer and activator of transcription 3 (STAT3) were determined by Western blot. The mRNA and protein expressions of Bcl-2

Bax

Cyclin D1 and Cyclin-dependent kinase 4 (CDK4) in HCC tumor tissues were respectively determined by reverse transcription polymerase chain reaction (RT-PCR) and Western blot. The protein expression of vascular endothelial growth factor A (VEGF-A) in tumor tissues was examined by IHC staining.

Results:

BBP treatment led to a significant decrease on tumor volume and tumor weight in HCC mice (

0.05) and had no effect on the change of body weight. In addition

BBP profoundly promoted cell apoptosis

inhibited cell proliferation and intratumoral microvessel density in HCC tumor tissues (

0.05). Moreover

BBP treatment remarkably suppressed the STAT3 phosphorylation and modulated the expression of critical target genes including Bcl-2

Bax

Cyclin D1

CDK4 and VEGF-A in HCC mice.

Conclusion:

BBP exerts its anti-cancer activities via suppressing STAT3 signaling pathway and affecting multiple intracellular targets.

关键词

Keywords

bear bile powderChinese medicinehepatocellular carcinomasignal transducer and activator of transcription 3 pathway

references

More>

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Safflower Yellow Inhibits Progression of Hepatocellular Carcinoma by Modulating Immunological Tolerance via FAK Pathway

Chemical Composition, Pharmacological Effects and Clinical Applications of Cinobufacini

Immunomodulatory Function of Pien Tze Huang in T Cell-Mediated Anti-tumor Activity against B16-F10, MC38 and Hep1-6 Tumor Models

Immunoregulatory and Anti-cancer Activities of Combination Treatment of Novel Four-Herb Formula and Doxorubicin in 4T1-Breast Cancer Bearing Mice

TRAIL and Celastrol Combinational Treatment Suppresses Proliferation, Migration, and Invasion of Human Glioblastoma Cells via Targeting Wnt/β-Catenin Signaling Pathway