Objective:

2

To observe the changes of ischemic myocardial cells apoptosis in rats following intervention with Xuefu Zhuyu Oral Liquid (血府逐瘀口服液

XFZY)

as well as changes of protein expression of silent information regulator 1 (SIRT1) and SIRT1 pathway-related genes.

Methods:

2

H9c2 rat myocardial cells were divided into 6 groups: control group

oxygen glucose deprivation (OGD) group

SIRT1 siRNA group

OGD+SIRT1 siRNA group

OGD+XFZY group

and OGD+SIRT1 siRNA+XFZY group. Quantitative fluorescent polymerase chain reaction (PCR) and Western blot were used to detect the concentration variations of SIRT1 and its pathway-related genes and corresponding protein expression after XFZY intervention and SIRT1 transfection.

Results:

2

Compared with the control group

the mRNA and protein expressions of SIRT1 were decreased obviously

while the mRNA and protein levels of P53

forkhead box protein O1 (FoxO1)

FoxO3

FoxO4 and nuclear factor kappa B (NF-κB) were increased in the OGD group

SIRT1 siRNA group

and OGD+SIRT1 siRNA group (

P

<

0.01). Compared with the OGD group and OGD+SIRT1 siRNA group

the treatment of XFZY inhibited the decline in SIRT1 mRNA and protein expressions (

P

<

0.01)

and down-regulated the mRNA and protein levels of P53

FoxO1

FoxO3

FoxO4 and NF-κB

respectively (

P

<

0.05 or

P

<

0.01).

Conclusion:

2

XFZY could prevent myocardial cells apoptosis probably by increasing the mRNA and protein expressions of SIRT1 and inhibiting the mRNA and protein expressions of P53

NF-κB

FoxO1

FoxO3 and FoxO4.