<italic style="font-style: italic">Hydrangeae Dulcis Folium</italic> Attenuates Physical Stress by Supressing ACTH-Induced Cortisol in Zebrafish

Junyoung Oh; Dong Hyun Kim; Gi-young Kim; Eun-Jin Park; Jong Hoon Ryu; Ji Wook Jung; Se Jin Park; Gwang-Woo Kim; Seungheon Lee

doi:10.1007/s11655-019-3204-6

Your Location：

Home >

Browse articles >

Hydrangeae Dulcis Folium Attenuates Physical Stress by Supressing ACTH-Induced Cortisol in Zebrafish

Original Article | Updated：2021-08-23

- Hydrangeae Dulcis Folium Attenuates Physical Stress by Supressing ACTH-Induced Cortisol in Zebrafish
- Hydrangeae Dulcis Folium Attenuates Physical Stress by Supressing ACTH-Induced Cortisol in Zebrafish
- Chinese Journal of Integrative Medicine 2020年26卷第2期页码：130-137
- Affiliations：
  
  1.Department of Marine Life Science, College of Ocean Science, Jeju National University, Jeju (63243), Republic of Korea
  2.Department of Medicinal Biotechnology, College of Health Sciences, Dong-A University, Busan (604-714), Republic of Korea
  3.Department of Food Bioengineering, Jeju National University, Jeju (63243), Republic of Korea
  4.Institute of Convergence Bio-Health, Dong-A University, Busan (604-714), Republic of Korea
  5.Department of Oriental Pharmaceutical Science, College of Pharmacy, Kyung Hee University, Hoeki-dong, Dongdaemoon-Ku, Seoul (130-701), Republic of Korea
  6.Kyung Hee East-West Pharmaceutical Research Institute, College of Pharmacy, Kyung Hee University, Hoeki-dong, Dongdaemoon-Ku, Seoul (130-701), Republic of Korea
  7.Department of Herbal Medicinal Pharmacology, College of Herbal Bio-industry, Daegu Haany University, Yugok-dong, Gyeongsan (712-715), Republic of Korea
  8.School of Natural Resources and Environmental Sciences, Kangwon National University, Chuncheon (24341), Republic of Korea
  9.Marine Bio Convergence Center, Gangneung Science & Industry Promotion Agency, Gangneung (25440), Republic of Korea
- Author bio：
  
  Correspondence to: Prof. Seungheon Lee, E-mail:slee76@jejunu.ac.kr
- Funds：
  
  the Ministry of Trade, Industry & Energy(MOTIE);Korea Institute for Advancement of Technology (KIAT) through the Encouragement Program for the Industries of Economic Cooperation Region
- DOI：10.1007/s11655-019-3204-6
  中图分类号：
- 纸质出版日期：2020-02-01，
  
  网络出版日期：2019-12-23，
  
  录用日期：2018-01-31
Scan for full text
Junyoung Oh, Dong Hyun Kim, Gi-young Kim, 等. Hydrangeae Dulcis Folium Attenuates Physical Stress by Supressing ACTH-Induced Cortisol in Zebrafish[J]. Chinese Journal of Integrative Medicine, 2020,26(2):130-137.

Junyoung Oh, Dong Hyun Kim, Gi-young Kim, et al. Hydrangeae Dulcis Folium Attenuates Physical Stress by Supressing ACTH-Induced Cortisol in Zebrafish[J]. Chinese Journal of Integrative Medicine, 2020,26(2):130-137.
Junyoung Oh, Dong Hyun Kim, Gi-young Kim, 等. Hydrangeae Dulcis Folium Attenuates Physical Stress by Supressing ACTH-Induced Cortisol in Zebrafish[J]. Chinese Journal of Integrative Medicine, 2020,26(2):130-137. DOI： 10.1007/s11655-019-3204-6.

Junyoung Oh, Dong Hyun Kim, Gi-young Kim, et al. Hydrangeae Dulcis Folium Attenuates Physical Stress by Supressing ACTH-Induced Cortisol in Zebrafish[J]. Chinese Journal of Integrative Medicine, 2020,26(2):130-137. DOI： 10.1007/s11655-019-3204-6.

摘要

Abstract

Objective:

To determine the effects of

Hydrangeae Dulcis Folium

(EHDF) on physical stress

changes in the whole-body cortisol level and behaviour in zebrafish (

Danio rerio

Methods:

One hundred and seventy-four fish were randomly divided into 4 [adrenocorticotropin hormone (ACTH) challenge test: 4 fish per group] or 6 groups (behavioural test: 10–12 fish per group

whole-body cortisol: 4 fish per group). Net handling stress (NHS) was used to induce physical stress. Fish were treated with vehicle or EHDF (5–20 mg/L) for 6 min before they were exposed to stress. And then

fish were sacrificed for collecting body fluid from whole-body or conducted behavioural tests

including novel tank test and open field test

and were evaluated to observe anxiety-like behaviours and locomotion. In addition

to elucidate the mode of action of the anti-stress effects of EHDF

ACTH (0.2 IU/g

i.p.) challenge test was performed.

Results:

The increased anxiety-like behaviours in novel tank test and open field test under stress were prevented by treatment with EHDF at 5–20 mg/L (

0.05). Moreover

compared with the unstressed group

which was not treated with NHS

the whole-body cortisol level was significantly increased by treatment with NHS (

0.05). Compared with the NHS-treated stressed control group

pre-treatment with EHDF at concentrations of 5–20 mg/L for 6 min significantly prevented the NHS-increased whole-body cortisol level (

0.05). In addition

ACTH challenge test showed that EHDF completely blocked the effects of ACTH on cortisol secretion (

0.05).

Conclusion:

EHDF may be a good antistress candidate and its mechanism of action may be related to its positive effects on cortisol release.

关键词

Keywords

net handling stresszebrafishHydrangeae Dulcis Foliumwhole-body cortisolbehavioural tests

references

More>

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Ethyl Lithospermate Reduces Lipopolysaccharide-Induced Inflammation through Inhibiting NF-κB and STAT3 Pathways in RAW 264.7 Cells and Zebrafish

Radix Panacis quinquefolii Extract Ameliorates Inflammatory Bowel Disease through Inhibiting Inflammation

Genotoxicity and Embryotoxicity Study of Bicyclol Methyl Ether, Main Impurity in Bicyclol

Hydrangeae Dulcis Folium Attenuates Physical Stress by Supressing ACTH-Induced Cortisol in Zebrafish

Hydrangeae Dulcis Folium Attenuates Physical Stress by Supressing ACTH-Induced Cortisol in Zebrafish

DOI：10.1007/s11655-019-3204-6

摘要

Abstract

关键词

Keywords

references