Electroacupuncture Attenuates Surgical Stress-Induced Reduction of T Lymphocytes through Modulation of Peripheral Opioid System

Ke WANG; Yue YONG; Jia ZHOU; Wen-xiong ZHOU; Jun GUO; Tong-yu CHEN

doi:10.1007/s11655-020-3158-8

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Electroacupuncture Attenuates Surgical Stress-Induced Reduction of T Lymphocytes through Modulation of Peripheral Opioid System

Acupuncture Research | Updated：2021-08-16

- Electroacupuncture Attenuates Surgical Stress-Induced Reduction of T Lymphocytes through Modulation of Peripheral Opioid System
- Electroacupuncture Attenuates Surgical Stress-Induced Reduction of T Lymphocytes through Modulation of Peripheral Opioid System
- Chinese Journal of Integrative Medicine 2021年27卷第2期页码：98-105
- Affiliations：
  
  1.Department of Cardiothoracic Surgery, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai(200437), China
  2.Institute of Clinical Immunology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai (200437), China
  3.Research Institute of Acupuncture Anesthesia, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai (201203), China
  4.Department of Anesthesiology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai (201203), China
- Author bio：
  
  Correspondence to: Prof. CHEN Tong-yu, E-mail: tonychen080@sina.com
- Funds：
  
  the National Natural Science Foundation of China;Medical Guidance Project of the Shanghai Science and Technology Commission;research grant from Shanghai Hospital Development Center;The Interdisciplinary Project of "Clinical Immunology of Traditional Chinese Medicine" in Shanghai
- DOI：10.1007/s11655-020-3158-8
  中图分类号：
- 纸质出版日期：2021-02-01，
  
  网络出版日期：2020-09-26，
  
  录用日期：2018-11-05
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Ke WANG, Yue YONG, Jia ZHOU, 等. Electroacupuncture Attenuates Surgical Stress-Induced Reduction of T Lymphocytes through Modulation of Peripheral Opioid System[J]. Chinese Journal of Integrative Medicine, 2021,27(2):98-105.

Ke WANG, Yue YONG, Jia ZHOU, et al. Electroacupuncture Attenuates Surgical Stress-Induced Reduction of T Lymphocytes through Modulation of Peripheral Opioid System[J]. Chinese Journal of Integrative Medicine, 2021,27(2):98-105.
Ke WANG, Yue YONG, Jia ZHOU, 等. Electroacupuncture Attenuates Surgical Stress-Induced Reduction of T Lymphocytes through Modulation of Peripheral Opioid System[J]. Chinese Journal of Integrative Medicine, 2021,27(2):98-105. DOI： 10.1007/s11655-020-3158-8.

Ke WANG, Yue YONG, Jia ZHOU, et al. Electroacupuncture Attenuates Surgical Stress-Induced Reduction of T Lymphocytes through Modulation of Peripheral Opioid System[J]. Chinese Journal of Integrative Medicine, 2021,27(2):98-105. DOI： 10.1007/s11655-020-3158-8.

摘要

Abstract

Objective:

To investigate the action mechanisms of electroacupuncture (EA) on postoperative immunosuppression.

Methods:

Male C57BL/6 mice (5–7 weeks old) were randomly divided into: the sham injury group

the surgical trauma stressed group

the EA group [surgery + 2/100 Hz EA at Neiguan (PC 6)]

and the EA+ Nal (surgery + EA + intraperitoneal injection of naloxone). Abdominal surgical trauma stress mice model was established. EA was performed on bilateral PC 6 acupoints by an EA apparatus (2/100 Hz) for 20 min once a day for 3 days. The mRNA expressions of MOR

DOR

and KOR in thymus and L3–L5 dorsal root ganglions (DRG) were determined by quantitative real-time polymerase chain reaction (qRT-PCR) and the protein expressions of MOR

DOR

and KOR in thymus were measured by Western blot. Flow cytometry assay was used to detect the levels of T lymphocyte subtypes in the peripheral blood.

Results:

Surgical trauma induced decreased the mRNA expression level of MOR in both thymus (

0.01) and L3–L5 DRGs (

0.05). Moreover

EA treatment not only significantly attenuated the MOR protein and mRNA expression in the thymus (both

0.05)

but also markedly increased expression of DOR and KOR opioid receptor in thymus (

0.01). However

the mRNA expressions of opioid receptors were not regulated by EA in the DRG (all

0.05). Furthermore

T lymphocyte population of CD

and CD

was decreased in the peripheral blood after surgical trauma (both

0.01). EA treatment can significantly elevate the population of CD

(

0.01)

(

0.05) and CD

T cells (

0.01). Intraperitoneal injection of the non-selective opioid receptor antagonist naloxone blocked the up-regulation of T lymphocytes by EA.

Conclusion:

EA may improve postoperative immunosuppression through the peripheral opioid system.

关键词

Keywords

electroacupunctureChinese medicineimmunosuppressionopioid receptorT-lymphocytes

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