<italic style="font-style: italic">Panax quinquefolium</italic> saponin Optimizes Energy Homeostasis by Modulating AMPK-Activated Metabolic Pathways in Hypoxia-Reperfusion Induced Cardiomyocytes

Yong-hui YU; Pei ZHANG; Cheng-long WANG; Jian-gang LIU; Peng LI; Da-wu ZHANG

doi:10.1007/s11655-020-3194-4

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Panax quinquefolium saponin Optimizes Energy Homeostasis by Modulating AMPK-Activated Metabolic Pathways in Hypoxia-Reperfusion Induced Cardiomyocytes

Original Article | Updated：2021-09-01

- Panax quinquefolium saponin Optimizes Energy Homeostasis by Modulating AMPK-Activated Metabolic Pathways in Hypoxia-Reperfusion Induced Cardiomyocytes
- Panax quinquefolium saponin Optimizes Energy Homeostasis by Modulating AMPK-Activated Metabolic Pathways in Hypoxia-Reperfusion Induced Cardiomyocytes
- Chinese Journal of Integrative Medicine 2021年27卷第8期页码：613-620
- Affiliations：
  
  1.Department of Traditional Chinese Medicine, China-Japan Friendship Hospital, Beijing(100029), China
  2.Graduate Shcool, Beijing University of Chinese Medicine, Beijing(100029), China
  3.Department of Cardiovascular Disease, Mongolian Hospital of Traditional Chinese Medicine, Wuhai, Inner Mongolia Autonomous Region (016000), China
  4.Center for Cardiovascular Diseases, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing(100091), China
- Author bio：
  
  Dr. WANG Cheng-long, E-mail:wcl796@163.com
- Funds：
  
  the National Natural Science Foundation of China(81273934;81874410)
- DOI：10.1007/s11655-020-3194-4
  中图分类号：
- 纸质出版日期：2021-08-01，
  
  网络出版日期：2020-05-16，
  
  录用日期：2019-09-12
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Yong-hui YU, Pei ZHANG, Cheng-long WANG, 等. Panax quinquefolium saponin Optimizes Energy Homeostasis by Modulating AMPK-Activated Metabolic Pathways in Hypoxia-Reperfusion Induced Cardiomyocytes[J]. Chinese Journal of Integrative Medicine, 2021,27(8):613-620.

Yong-hui YU, Pei ZHANG, Cheng-long WANG, et al. Panax quinquefolium saponin Optimizes Energy Homeostasis by Modulating AMPK-Activated Metabolic Pathways in Hypoxia-Reperfusion Induced Cardiomyocytes[J]. Chinese Journal of Integrative Medicine, 2021,27(8):613-620.
Yong-hui YU, Pei ZHANG, Cheng-long WANG, 等. Panax quinquefolium saponin Optimizes Energy Homeostasis by Modulating AMPK-Activated Metabolic Pathways in Hypoxia-Reperfusion Induced Cardiomyocytes[J]. Chinese Journal of Integrative Medicine, 2021,27(8):613-620. DOI： 10.1007/s11655-020-3194-4.

Yong-hui YU, Pei ZHANG, Cheng-long WANG, et al. Panax quinquefolium saponin Optimizes Energy Homeostasis by Modulating AMPK-Activated Metabolic Pathways in Hypoxia-Reperfusion Induced Cardiomyocytes[J]. Chinese Journal of Integrative Medicine, 2021,27(8):613-620. DOI： 10.1007/s11655-020-3194-4.

摘要

Abstract

Objective:

To investigate the effects and underlying mechanisms of

Panax quinquefolium

saponin (PQS) on energy deficiency in hypoxia-reperfusion (H/R) induced cardiomyocytes.

Methods:

The H/R injury involved hypoxia for 3 h and then reperfusion for 2 h. Cardiomyocytes recruited from neonatal rat ventricular myocytes (NRVMs) were randomly divided into control

H/R

H/R+compound C (C.C)

H/R+PQS

and H/R+C.C+PQS groups. BrdU assay

lactase dehydrogenase (LDH) leakage and early apoptosis rate were evaluated to assess cell damages. Contents of high energy phosphate compounds were conducted to detect the energy production. Protein expression levels of adenosine monophosphate-activated protein kinase α (AMPKα)

glucose transporter 4 (GLUT4)

phosphate fructose kinase 2 (PFK2)

fatty acid translocase/cluster of differentiation 36 (FAT/CD36)

and acetyl CoA carboxylase 2 (ACC2) in the regulatory pathways were measured by Western blotting. Immunofluorescence staining of GLUT4 and FAT/CD36 was used to observe the mobilization of metabolic transporters.

Results:

PQS (50 mg/L) pretreatment significantly alleviated H/R-induced inhibition of NRVMs viability

up-regulation of LDH leakage

acceleration of early apoptosis

and reduction of energy production (

0.05). Compared with the H/R group

up-regulated expression of AMPKα

GLUT4

PFK2

FAT/CD36 and ACC2 were observed

and more GLUT4 and FAT/CD36 expressions were detected on the membrane in the H/R+PQS group (

0.05). These effects of PQS on H/R-induced NRVMs were eliminated in the H/R+C.C+PQS group (

0.05).

Conclusion:

PQS has prominent advantages in protecting NRVMs from H/R-induced cell damages and energy metabolic disorders

by activation of AMPKα-mediated GLUT4-PFK2 and FAT/CD36-ACC2 pathways.

关键词

Keywords

Panax quinquefolium saponinChinese medicineneonatal rat ventricular myocyteshypoxiareperfusionenergy metabolismadenosine monophosphate-activated protein kinase αpathways

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Panax quinquefolium saponin Optimizes Energy Homeostasis by Modulating AMPK-Activated Metabolic Pathways in Hypoxia-Reperfusion Induced Cardiomyocytes

Panax quinquefolium saponin Optimizes Energy Homeostasis by Modulating AMPK-Activated Metabolic Pathways in Hypoxia-Reperfusion Induced Cardiomyocytes

DOI：10.1007/s11655-020-3194-4

摘要

Abstract

关键词

Keywords

references