Protective Effect of Naoxintong Capsule (脑心通胶囊) Combined with Guhong Injection (谷红注射液) on Rat Brain Microvascular Endothelial Cells during Cerebral Ischemia-Reperfusion Injury*

Hai-yan WANG; Hui-fen ZHOU; Yu HE; Li YU; Chang LI; Jie-hong YANG; Hai-tong WAN

doi:10.1007/s11655-020-3215-3

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Protective Effect of Naoxintong Capsule (脑心通胶囊) Combined with Guhong Injection (谷红注射液) on Rat Brain Microvascular Endothelial Cells during Cerebral Ischemia-Reperfusion Injury*

Original Article | Updated：2021-10-18

- Protective Effect of Naoxintong Capsule (脑心通胶囊) Combined with Guhong Injection (谷红注射液) on Rat Brain Microvascular Endothelial Cells during Cerebral Ischemia-Reperfusion Injury*
- Protective Effect of Naoxintong Capsule (脑心通胶囊) Combined with Guhong Injection (谷红注射液) on Rat Brain Microvascular Endothelial Cells during Cerebral Ischemia-Reperfusion Injury*
- Chinese Journal of Integrative Medicine 2021年27卷第10期页码：744-751
- Affiliations：
  
  1.College of Life Science, Zhejiang Chinese Medical University,Hangzhou (310053), China
  2.College of Pharmacy, Zhejiang Chinese Medical University, Hangzhou (310053), China
  3.College of Basic Medicine, Zhejiang Chinese Medical University,Hangzhou (310053), China
- Author bio：
  
  Prof. WAN Hai-tong, E-mail: whtong@126.com
- Funds：
  
  the National Natural Science Foundation of China(81630105;81973560);Zhejiang Provincial Natural Science Foundation of China(LZ17H270001;LZ18H270001);Zhejiang Provincial Program for the Cultivation of High-level Innovative Health Talents
- DOI：10.1007/s11655-020-3215-3
  中图分类号：
- 纸质出版日期：2021-10-01，
  
  网络出版日期：2020-04-4，
  
  录用日期：2019-08-31
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Hai-yan WANG, Hui-fen ZHOU, Yu HE, 等. Protective Effect of Naoxintong Capsule (脑心通胶囊) Combined with Guhong Injection (谷红注射液) on Rat Brain Microvascular Endothelial Cells during Cerebral Ischemia-Reperfusion Injury*[J]. Chinese Journal of Integrative Medicine, 2021,27(10):744-751.

Hai-yan WANG, Hui-fen ZHOU, Yu HE, et al. Protective Effect of Naoxintong Capsule (脑心通胶囊) Combined with Guhong Injection (谷红注射液) on Rat Brain Microvascular Endothelial Cells during Cerebral Ischemia-Reperfusion Injury*[J]. Chinese Journal of Integrative Medicine, 2021,27(10):744-751.
Hai-yan WANG, Hui-fen ZHOU, Yu HE, 等. Protective Effect of Naoxintong Capsule (脑心通胶囊) Combined with Guhong Injection (谷红注射液) on Rat Brain Microvascular Endothelial Cells during Cerebral Ischemia-Reperfusion Injury*[J]. Chinese Journal of Integrative Medicine, 2021,27(10):744-751. DOI： 10.1007/s11655-020-3215-3.

Hai-yan WANG, Hui-fen ZHOU, Yu HE, et al. Protective Effect of Naoxintong Capsule (脑心通胶囊) Combined with Guhong Injection (谷红注射液) on Rat Brain Microvascular Endothelial Cells during Cerebral Ischemia-Reperfusion Injury*[J]. Chinese Journal of Integrative Medicine, 2021,27(10):744-751. DOI： 10.1007/s11655-020-3215-3.

摘要

Abstract

Objective:

To investigate the synergistic effect of Naoxintong Capsule (NXTC

脑心通胶囊) and Guhong Injection (GHI

谷红注射液) on cerebral ischemia-reperfusion (I/R) injury.

Methods:

Forty-eight Sprague-Dawley rats were divided into 6 groups: control group

oxygen and glucose deprivation (OGD) group

nimodipine group (9.375 mg/kg)

NXTC group (0.5 g/kg)

GHI group (5 mL/kg) and NXTC+GHI group (0.5 g/kg NXTC+5 mL/kg GHI)

after the onset of reperfusion and once per day for the following 7 days. Blood was collected 1 h after final administration

and the sera were collected. Cultured primary rat brain microvascular endothelial cells (rBMECs) were subjected to OGD to establish a cell injury model. Untreated rBMECs were used as blank control. The cell counting kit-8 assay was used to assess cell viability using the sera. Malondialdehyde (MDA) and superoxide dismutase (SOD) levels were assessed using an enzymelinked immunosorbent assay. Apoptosis was evaluated after Hoechst33342 staining using fluorescence microscopy and flow cytometry. JC-1 staining was performed to assess changes in mitochondrial membrane potential.

Results:

Statistical analysis indicated that more than 95% of the cells were rBMECs. Compared with the OGD group

the cellular morphology of the all drug delivery groups improved. In particular

the combined drug group had the most significant effect. Compared with the OGD group

all drug intervention groups induced a decrease in the apoptotic rate of rBMECs

increased the SOD levels

and decreased the MDA levels (all

0.01). Compared with the mono-therapy groups

the NXTC+GHI group exhibited a significant improvement in the number of apoptotic rBMECs (

0.01). All drug intervention groups showed different degrees of increase in membrane potential

and the NXTC+GHI group was higher than the NXTC or GHI group (

0.01).

Conclusion:

The combinationa application of NXTC and GHI on cerebral I/R injury clearly resulted in protective benefits.

关键词

Keywords

cerebral ischemia/reperfusion injuryNaoxintong CapsuleGuhong Injectionbrain microvascular endothelial cellsapoptosisrat

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