Effect of Electro-acupuncture on Expression of IRS-1/PI3K/GLUT4 Pathway in Ovarian Granulosa Cells of Infertile Patients with Polycystic Ovary Syndrome-Insulin Resistance of Phlegm-Dampness Syndrome*

Shan XIANG; Ming-feng XIA; Jing-yan SONG; Dan-qi LIU; Fang LIAN

doi:10.1007/s11655-020-3219-z

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Effect of Electro-acupuncture on Expression of IRS-1/PI3K/GLUT4 Pathway in Ovarian Granulosa Cells of Infertile Patients with Polycystic Ovary Syndrome-Insulin Resistance of Phlegm-Dampness Syndrome*

Original Article | Updated：2021-09-01

- Effect of Electro-acupuncture on Expression of IRS-1/PI3K/GLUT4 Pathway in Ovarian Granulosa Cells of Infertile Patients with Polycystic Ovary Syndrome-Insulin Resistance of Phlegm-Dampness Syndrome*
- Effect of Electro-acupuncture on Expression of IRS-1/PI3K/GLUT4 Pathway in Ovarian Granulosa Cells of Infertile Patients with Polycystic Ovary Syndrome-Insulin Resistance of Phlegm-Dampness Syndrome*
- Chinese Journal of Integrative Medicine 2021年27卷第5期页码：330-335
- Affiliations：
  
  1.Gynecology of Traditional Chinese Medicine, the First Clinical College, Shandong University of Traditional Chinese Medicine, Jinan (250355), China
  2.Reproductive and Genetic Center of Integrated Traditional and Western Medicine, the Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan (250011), China
  3.Experimental Center, Shandong University of Traditional Chinese Medicine, Jinan (250355), China
- Author bio：
  
  Prof. LIAN Fang, E-mail: lianfangbangong@163.com
- Funds：
  
  the Shandong Natural Science Foundation(ZR2019QH013);Shandong Natural Science Foundation(2018CXGC130);State Administration of Foreign Experts Affairs, China(P192023031)
- DOI：10.1007/s11655-020-3219-z
  中图分类号：
- 纸质出版日期：2021-05-01，
  
  网络出版日期：2020-06-22，
  
  录用日期：2020-02-06
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Shan XIANG, Ming-feng XIA, Jing-yan SONG, 等. Effect of Electro-acupuncture on Expression of IRS-1/PI3K/GLUT4 Pathway in Ovarian Granulosa Cells of Infertile Patients with Polycystic Ovary Syndrome-Insulin Resistance of Phlegm-Dampness Syndrome*[J]. Chinese Journal of Integrative Medicine, 2021,27(5):330-335.

Shan XIANG, Ming-feng XIA, Jing-yan SONG, et al. Effect of Electro-acupuncture on Expression of IRS-1/PI3K/GLUT4 Pathway in Ovarian Granulosa Cells of Infertile Patients with Polycystic Ovary Syndrome-Insulin Resistance of Phlegm-Dampness Syndrome*[J]. Chinese Journal of Integrative Medicine, 2021,27(5):330-335.
Shan XIANG, Ming-feng XIA, Jing-yan SONG, 等. Effect of Electro-acupuncture on Expression of IRS-1/PI3K/GLUT4 Pathway in Ovarian Granulosa Cells of Infertile Patients with Polycystic Ovary Syndrome-Insulin Resistance of Phlegm-Dampness Syndrome*[J]. Chinese Journal of Integrative Medicine, 2021,27(5):330-335. DOI： 10.1007/s11655-020-3219-z.

Shan XIANG, Ming-feng XIA, Jing-yan SONG, et al. Effect of Electro-acupuncture on Expression of IRS-1/PI3K/GLUT4 Pathway in Ovarian Granulosa Cells of Infertile Patients with Polycystic Ovary Syndrome-Insulin Resistance of Phlegm-Dampness Syndrome*[J]. Chinese Journal of Integrative Medicine, 2021,27(5):330-335. DOI： 10.1007/s11655-020-3219-z.

摘要

Abstract

Objective:

To evaluate the effect of electro-acupuncture (EA) in infertile patients with phlegmdampness polycystic ovary syndrome-insulin resistance (PCOS-IR).

Methods:

Seventy-six PCOS-IR patients who underwnet

in vitro

fertilization and embryo transfer (IVF-ET) were equally assigned to two groups according to a random digital table: the EA group and the control group

with 38 cases in each group. Before undergoing IVF

the two groups were treated with EA or pseudo-acupuncture

respectively

for 3 menstrual cycles. The intervention was 25 min twice a week until the day of oocyte collection. The selected acupoints were Zhongwan (RN 12)

Tianshu (ST 25)

Daheng (SP 15)

Daimai (GB 26)

Qihai (CV 6)

Guanyuan (CV 4)

and bilateral points including Xuehai (SP 10)

Fenglong (ST 40)

Zusanli (ST 36)

and Yinlingquan (SP 9). Evaluation of phlegm-dampness syndrome score and IR score were carried out before and after treatment. Additionally

the number of oocytes retrieved

transplantable embryo rate

high-quality embryo rate

clinical pregnancy rate and live birth rate were compared between the two groups. Real-time polymerase chain reaction analysis was used to monitor the mRNA expression of the insulin receptor substrate (IRS-1)

phosphatidylinositiol 3-kinase (PI3K) and glucose transport factor 4 (GLUT4) in ovarian granulosa cells.

Results:

EA treatment reduced the phlegm-dampness syndrome score as well as the IR scores compared with the control group (

0.05). No significant differences in the number of oocytes retrieved and clinical pregnancy rate between the two groups (

0.05). Moreover

the transplantable embryo rate [49.0% (284/580) vs. 41.9% (273/652)]

high-quality embryo rate [36.6% (104/284) vs. 27.8% (76/273)]

and live birth rate [50% (19/38) vs. 26.3% (10/38)] in the EA group were significantly higher than in the control group (

0.05). Gene expression analyses revealed significantly elevated IRS-1

PI3K and GLUT4 mRNA in ovarian granulosa cells of the EA group compared with the control group (

0.05).

Conclusions:

EA may ameliorate the effects of phlegm-dampness syndrome and ovarian IR in PCOS-IR patients. Mechanistically

this effect might be through an upregulation of the IRS-1/PI3K/GLUT4 signaling pathway

which may result in improved oocyte quality and embryonic development potential. (Registration No. ChiCTR1800015453)

关键词

Keywords

polycystic ovary syndromephlegm-dampness syndromeelectro-acupunctureinsulin resistancegranulosa cellsmechanism of action

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