Antidepressant-Like Effects of Chaihu Shugan Powder(柴胡疏肝散) on Rats Exposed to Chronic Unpredictable Mild Stress through Inhibition of Endoplasmic Reticulum Stress-Induced Apoptosis*

Ke-huan SUN; Yu JIN; Zhi-gang MEI; Zhi-tao FENG; Jie-ren LIU; Mei-qun CAO; Zheng-zhi WU

doi:10.1007/s11655-020-3228-y

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Antidepressant-Like Effects of Chaihu Shugan Powder(柴胡疏肝散) on Rats Exposed to Chronic Unpredictable Mild Stress through Inhibition of Endoplasmic Reticulum Stress-Induced Apoptosis*

Original Articles | Updated：2021-09-01

- Antidepressant-Like Effects of Chaihu Shugan Powder(柴胡疏肝散) on Rats Exposed to Chronic Unpredictable Mild Stress through Inhibition of Endoplasmic Reticulum Stress-Induced Apoptosis*
- Antidepressant-Like Effects of Chaihu Shugan Powder(柴胡疏肝散) on Rats Exposed to Chronic Unpredictable Mild Stress through Inhibition of Endoplasmic Reticulum Stress-Induced Apoptosis*
- Chinese Journal of Integrative Medicine 2021年27卷第5期页码：353-360
- Affiliations：
  
  1.The First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, Shenzhen, Guangdong Province(518020), China
  2.Third- Grade Pharmacological Laboratory on Chinese Medicine Approved by State Administration of Traditional Chinese Medicine, Medical College of China Three Gorges University, Yichang, Hubei Province (443002), China
- Author bio：
  
  Prof. WU Zheng-zhi, E-mail:szwzz001@163.com.
- Funds：
  
  National Natural Science Foundation of China(81574038);Shenzhen Science and Technology Program(JCYJ20180508152437368);Sanming Project of Medicine in Shenzhen, China(SZSM201612049)
- DOI：10.1007/s11655-020-3228-y
  中图分类号：
- 纸质出版日期：2021-05-01，
  
  网络出版日期：2020-06-22，
  
  录用日期：2019-02-24
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Ke-huan SUN, Yu JIN, Zhi-gang MEI, 等. Antidepressant-Like Effects of Chaihu Shugan Powder(柴胡疏肝散) on Rats Exposed to Chronic Unpredictable Mild Stress through Inhibition of Endoplasmic Reticulum Stress-Induced Apoptosis*[J]. Chinese Journal of Integrative Medicine, 2021,27(5):353-360.

Ke-huan SUN, Yu JIN, Zhi-gang MEI, et al. Antidepressant-Like Effects of Chaihu Shugan Powder(柴胡疏肝散) on Rats Exposed to Chronic Unpredictable Mild Stress through Inhibition of Endoplasmic Reticulum Stress-Induced Apoptosis*[J]. Chinese Journal of Integrative Medicine, 2021,27(5):353-360.
Ke-huan SUN, Yu JIN, Zhi-gang MEI, 等. Antidepressant-Like Effects of Chaihu Shugan Powder(柴胡疏肝散) on Rats Exposed to Chronic Unpredictable Mild Stress through Inhibition of Endoplasmic Reticulum Stress-Induced Apoptosis*[J]. Chinese Journal of Integrative Medicine, 2021,27(5):353-360. DOI： 10.1007/s11655-020-3228-y.

Ke-huan SUN, Yu JIN, Zhi-gang MEI, et al. Antidepressant-Like Effects of Chaihu Shugan Powder(柴胡疏肝散) on Rats Exposed to Chronic Unpredictable Mild Stress through Inhibition of Endoplasmic Reticulum Stress-Induced Apoptosis*[J]. Chinese Journal of Integrative Medicine, 2021,27(5):353-360. DOI： 10.1007/s11655-020-3228-y.

摘要

Abstract

Objective:

To investigate the antidepressant-like effects of Chaihu Shugan Powder (CSP

柴胡疏肝散) and to explore its underlying mechanisms.

Methods:

Thirty-two Sprague-Dawley rats were randomly divided into control (CON)

chronic unpredictable mild stress (CUMS)

fluoxetine (FLU)

and CSP groups

8 rats in each group. All of the rats except for those in the control group were subjected to 3 consecutive weeks of CUMS to establish the depression model. The open field test (OFT)

forced swimming test (FST)

and sucrose preference test were used to assess the anti-anxiety and antidepressant effects of CSP. Terminal deoxynucleotidyl transferase (TdT) dUTP nick-end labeling was used to determine the apoptosis rate in the hippocampal tissues. The mRNA and protein levels of glucose-regulated protein (GRP) 78

spliced X-box-binding protein (XBP)-1

CCAAT/enhancerbinding protein homologous protein (CHOP)

caspase-12

and c-Jun N-terminal kinase (JNK) in the hippocampus of rats were evaluated by real-time PCR and Western blot analysis

respectively.

Results:

Administration of CSP alleviated anxiety and depression-like behavior in CUMS rats

as revealed by enhanced time and distance in the center of the OFT (

0.05)

an increased preference for sucrose

and longer swimming time and shorter immobility time during the FST (all

0.05). In addition

CSP treatment significantly reduced the rate of apoptosis in rat hippocampal neurons (

0.05). The mRNA and protein expression levels of GRP78

spliced XBP-1

and CHOP were down-regulated along with the expression of caspase-12 and cleaved caspase-12 proteins (all

0.05)

whereas total and phosphorylated JNK1 protein levels did not differ significantly between control and CSP-treated rats.

Conclusion:

CSP can improve depression-like behavior in rats exposed to CUMS

possibly by suppressing CHOP and caspase-12 mediated apoptosis in the rat hippocampus.

关键词

Keywords

depressionChaihu Shugan PowderChinese medicinechronic unpredictable mild stressendoplasmic reticulum stressapoptosis

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