Shenling Baizhu Powder (参苓白术散) Ameliorates Pi(Spleen)-Deficiency-Induced Functional Diarrhea in Rats

Yi XIAO; Kui ZHANG; Si-yu ZHU; Xiang-liang DENG; Xiao-yin CHEN; Nan-lin FU; Jia CHEN

doi:10.1007/s11655-020-3259-4

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Shenling Baizhu Powder (参苓白术散) Ameliorates Pi(Spleen)-Deficiency-Induced Functional Diarrhea in Rats

Original Article | Updated：2021-08-16

- Shenling Baizhu Powder (参苓白术散) Ameliorates Pi(Spleen)-Deficiency-Induced Functional Diarrhea in Rats
- Shenling Baizhu Powder (参苓白术散) Ameliorates Pi(Spleen)-Deficiency-Induced Functional Diarrhea in Rats
- Chinese Journal of Integrative Medicine 2021年27卷第3期页码：206-211
- Affiliations：
  
  1.Department of Chinese Medicine, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou (510062), China
  2.School of Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou (510006), China
  3.School of Chinese Medicine, Jinan University, Guangzhou (510632), China
- Author bio：
  
  Correspondence to: Prof. CHEN Jia, E-mail: tchenjia@gdpu.edu.cn
- Funds：
  
  the National Natural Science Foundation of China
- DOI：10.1007/s11655-020-3259-4
  中图分类号：
- 纸质出版日期：2021-03-01，
  
  网络出版日期：2020-07-27，
  
  录用日期：2020-01-2
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Yi XIAO, Kui ZHANG, Si-yu ZHU, 等. Shenling Baizhu Powder (参苓白术散) Ameliorates Pi(Spleen)-Deficiency-Induced Functional Diarrhea in Rats[J]. Chinese Journal of Integrative Medicine, 2021,27(3):206-211.

Yi XIAO, Kui ZHANG, Si-yu ZHU, et al. Shenling Baizhu Powder (参苓白术散) Ameliorates Pi(Spleen)-Deficiency-Induced Functional Diarrhea in Rats[J]. Chinese Journal of Integrative Medicine, 2021,27(3):206-211.
Yi XIAO, Kui ZHANG, Si-yu ZHU, 等. Shenling Baizhu Powder (参苓白术散) Ameliorates Pi(Spleen)-Deficiency-Induced Functional Diarrhea in Rats[J]. Chinese Journal of Integrative Medicine, 2021,27(3):206-211. DOI： 10.1007/s11655-020-3259-4.

Yi XIAO, Kui ZHANG, Si-yu ZHU, et al. Shenling Baizhu Powder (参苓白术散) Ameliorates Pi(Spleen)-Deficiency-Induced Functional Diarrhea in Rats[J]. Chinese Journal of Integrative Medicine, 2021,27(3):206-211. DOI： 10.1007/s11655-020-3259-4.

摘要

Abstract

Objective:

To explore the mechanism of Pi (Spleen)-deficiency-induced functional diarrhea (FD) model rats treated by Shenling Baizhu Powder (参苓白术散

SBP).

Methods:

Thirty male Sprague-Dawley rats were randomly divided into 5 groups including control

model

low-

medium-

and high-dose SBP groups (SBPLDG

SBPMDG

SBPHDG)

6 rats in each group

respectively. Pi-deficiency-induced FD rats model was developed through

Radix et Rhizoma

Rhei gavage for 7 days. After modeling

the rats were treated with 3 doses of SBP [0.93

1.86

and 3.72 g/(kg•d)]

and the rats in the control and model groups were given pure water for 7 days. The diarrhea index was calculated. On the 7th and 14th days

the traveled distance of rat was measured by the open field test. Serum D-xylose content was determined by the phloroglucinol method and interleukin (IL)-10 and IL-17 levels were measured using an enzyme-linked immunosorbent assay kit. The content of Treg cells was determined by flow cytometry.

Results:

Compared with the control group

the diarrhea index and IL-17 level in the model group were significantly higher and the total exercise distance and D-xylose content significantly decreased (

0.05). The expression of IL-10 in the SBPHDG group was significantly up-regulated

and serum D-xylose level and Treg cells increased significantly compared with the model group (

0.05).

Conclusion:

High-dose SBP exhibited ameliorating effects against Pi-deficiency induced FD

which might be attributed to its modulations on intestinal absorption function as well as adaptive immunity in mesenteric lymph nodes of rat.

关键词

Keywords

functional diarrheaPi (Spleen)-deficiencyShenling Baizhu PowderT helper type 17 cellsTreg cellsChinese medicine

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