Effect of Modified Xijiao Dihuang Decoction (加味犀角地黄汤) on Intestinal Flora and Th17/Treg in Rats with Radiation Enteritis

Yu-guo WANG; Yue GAO; Jian FENG; Yong-qi DOU

doi:10.1007/s11655-020-3261-x

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Effect of Modified Xijiao Dihuang Decoction (加味犀角地黄汤) on Intestinal Flora and Th17/Treg in Rats with Radiation Enteritis

Original Article | Updated：2021-08-16

- Effect of Modified Xijiao Dihuang Decoction (加味犀角地黄汤) on Intestinal Flora and Th17/Treg in Rats with Radiation Enteritis
- Effect of Modified Xijiao Dihuang Decoction (加味犀角地黄汤) on Intestinal Flora and Th17/Treg in Rats with Radiation Enteritis
- Chinese Journal of Integrative Medicine 2021年27卷第3期页码：198-205
- Affiliations：
  
  1.Medical School of Chinese People's Liberation Army, The General Hospital of People's Liberation Army, Beijing (100853), China
  2.Beijing Institute of Radiation Medicine, Beijing(100850), China
- Author bio：
  
  Correspondence to: Prof. DOU Yong-qi, E-mail: dyqi_301@yeah.net
- Funds：
  
  Logistics Research Project
- DOI：10.1007/s11655-020-3261-x
  中图分类号：
- 纸质出版日期：2021-03-01，
  
  网络出版日期：2020-04-1，
  
  录用日期：2020-01-14
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Yu-guo WANG, Yue GAO, Jian FENG, 等. Effect of Modified Xijiao Dihuang Decoction (加味犀角地黄汤) on Intestinal Flora and Th17/Treg in Rats with Radiation Enteritis[J]. Chinese Journal of Integrative Medicine, 2021,27(3):198-205.

Yu-guo WANG, Yue GAO, Jian FENG, et al. Effect of Modified Xijiao Dihuang Decoction (加味犀角地黄汤) on Intestinal Flora and Th17/Treg in Rats with Radiation Enteritis[J]. Chinese Journal of Integrative Medicine, 2021,27(3):198-205.
Yu-guo WANG, Yue GAO, Jian FENG, 等. Effect of Modified Xijiao Dihuang Decoction (加味犀角地黄汤) on Intestinal Flora and Th17/Treg in Rats with Radiation Enteritis[J]. Chinese Journal of Integrative Medicine, 2021,27(3):198-205. DOI： 10.1007/s11655-020-3261-x.

Yu-guo WANG, Yue GAO, Jian FENG, et al. Effect of Modified Xijiao Dihuang Decoction (加味犀角地黄汤) on Intestinal Flora and Th17/Treg in Rats with Radiation Enteritis[J]. Chinese Journal of Integrative Medicine, 2021,27(3):198-205. DOI： 10.1007/s11655-020-3261-x.

摘要

Abstract

Objective:

To observe the effect of Modified Xijiao Dihuang Decoction (加味犀角地黄汤

MXDD) on rats with radiation enteritis

and explore its action mechanism.

Methods:

Thirty female Sprague Dawley rats were divided into the control

model

dexamethasone (DXM)

golden bifid (GB) and MXDD groups using random number table

6 rats in each group. Except the control group

the other rats were developed into radiation enteritis model by exposing to a single

Co-γ ray at a dose of 11 Gy. The rats in the DXM

GB and MXDD groups were treated with DXM (1.425 mg/kg)

GB (0.8 g/kg) and MXDD (36.0 g/kg) once daily for 3 days

respectively. Body weight and diarrhea condition of rats were evaluated daily. On day 3

the feces of rats were collected for intestinal flora detection and the small intestinal tissues were also collected. Bacterial species annotation

alpha and beta diversities as well as composition of intestinal flora were detected and compared. The protein and mRNA expressions of interleukin 17 (IL-17)

retinoid-related orphan nuclear receptor gamma t (RORγt) and forkhead/ winged helix transcription factor p3 (FoxP3) were determined by Western blot and polymerase chain reaction

respectively. The abundance and diversity of intestinal flora as well as the proportion at the phylum and genus levels were assayed by 16S rRNA metagenome sequencing. Correlation between intestinal flora and Th17/Treg was analyzed by heatmap method.

Results:

On day 1 to 3 after radiation

compared with the control group

the body weight in model group was decreased (

0.05 or

0.01). Compared with the model group

MXDD could alleviate weight loss and diarrhea caused by irradiation. At the phylum level

MXDD cause a significant increase in

Firmicutes

and a decrease in

Proteobacteria

(

0.05 or

0.01). At the genus level

MXDD reduced the proportion of

Escherichia-Shigella

(

0.01). In addition

IL-17 and FoxP3 mRNA and protein expression levels were down-regulated and RORγt was up-regulated by MXDD treatment (

0.05). Besides

Firmicutes

and

Lactobacillus

were positively correlated with FoxP3 (

=0.73

0.79

respectively;

0.01)

negatively correlated with IL-17 (

=0.66

0.64

respectively;

0.01 or

0.05) and RORγt (

=0.73

0.81

respectively;

0.01).

Proteobacteria

and

Escherichia-Shigella

both had positive correlation with IL-17 (

=0.77

0.57

respectively;

0.01 or

0.05) and RORγt (

=0.94

0.79

respectively;

0.01) and negative correlation with FoxP3 (

=0.74

0.65;

0.01).

Conclusion:

MXDD could improve the survival status of irradiated rats by regulating the richness

diversity and composition of intestinal flora

and restoring the balance of Th17/Treg.

关键词

Keywords

Modified Xijiao Dihuang Decoctionradiation enteritisintestinal floraTh17/TregChinese medicine

references

Wang Y, Kong W, Lv N, Li F, Chen J, Jiao S, et al.Incidence of radiation enteritis in cervical cancer patients treated with definitive radiotherapyversus adjuvant radiotherapy. J Cancer Res Ther 2018;14:120-124.

Feng J, Wang YG, Xu CY, Xu R, Dou YQ. Study on the mechanism of clearing heat and detoxication and cooling blood method to improve blood coagulation and microcirculation in rats with acute radiation injury. China J Tradit Chin Med Pharm (Chin) 2019;34:355-358.

Wang Z, Wang Q, Wang X, Zhu L, Chen J, Zhang BL, et al.Gut microbial dysbiosis is associated with development and progression of radiation enteritis during pelvic radiotherapy.J Cell Mol Med 2019; 23:3747-3756.

Bessout R, Demarquay C, Moussa L, René A, Doix B, Benderitter M, et al. TH17 predominant T-cell responses in radiation-induced bowel disease are modulated by treatment with adipose-derived mesenchymal stromal cells.J Pathol 2015;237:435-446.

Hu HX, Sun J, Gao YJ, Fang H, Xu SQ, Dong J, et al.Effect of modified Wuzi Yanzong Pill on tip60-mediated apoptosis in testis of male rats after microwave radiation. Chin J Integr Med 2019;25:24-29.

Wang LJ, Lu JZ, Cai BN, Li MW, Qu BL. Effect of compound Zhuye Shigao Granule on acute radiation-induced esophagitis in cancer patients: a randomized controlled trial. Chin J Integr Med 2017;2:20-26.

Yang L, Yu MW, Wang XM, Zhang Y, Yang GW, Luo XQ, et al. Heijiangdan Ointment, relieves oxidative stress from radiation dermatitis induced by (60)Co γ-ray in mice. Chin J Integr Med 2016;22:110-115.

Cho HT, Kim JH, Heo W, Lee HS, Lee JJ, Park TS, et al.Explosively puffed Ginseng ameliorates ionizing radiation-induced injury of colon by decreasing oxidative stress-related apoptotic cell execution in mice. J Med Food 2019;22:490-498.

Wu C, Shan J, Feng J, Wang JL, Qin CB, Nie GX, et al. Effects of dietary Radix Rehmanniae Preparata polysaccharides on the growth performance, immune response and disease resistance of Luciobarbus capito.Fish Shellfish Immunol 2019;89:641-646.

Sun YF, Chen SQ, Wei RM, Xie X, Wang CC, Fan SH, et al. Metabolome and gut microbiota variations of long-term intake of Panax Ginseng extracts on rats. Food Function 2018;10:3547-3556.

Heo SB, Lim SW, Jhun JY, Cho ML, Chung BH, Yang CW. Immunological benefits by ginseng through reciprocal regulation of Th17 and Treg cells during cyclosporine-induced immunosuppression. J Ginseng Res 2016;40:18-27.

Song S, Chen D, Ma T, Luo Y, Yang Z, Wang D, et al.Molecular mechanism of acute radiation enteritis revealed using proteomics and biological signaling network analysis in rats. Dig Dis Sci 2014;59:2704-2713.

Wei W, ed. Pharmacology experimental methodology: new technology and new method. Beijing: People's Medical Publishing House 2010:69-70.

Kurita A, Kado S, Kaneda N, Onoue M, Hashimoto S, Yokokura T. Modified irinotecan hydrochloride (CPT-11)administration schedule improves induction of delayed-onset diarrhea in rats. Cancer Chemother Pharm 2000;46:211-220.

Chen YJ, Wu H, Wu SD, Lu N, Wang YT, Liu HN, et al.Parasutterella, in association with irritable bowel syndrome and intestinal chronic inflammation. J Gastroenterol Hepatol 2018;33:1844-1852.

Castellanos N, Diez GG, Antúnez-Almagro C, Bailén M, Bressa C, González Soltero R, et al. A critical mutualism-competition interplay underlies the loss of microbial diversity in sedentary lifestyle. Front Microbiol 2019;10:3142-3153.

Ao X, Jie S, Feng Z. Intestinal microbiota and kidney diseases. Chin J Integr Med 2018;24:8-10.

Zhang J, Wang C, Wang J, Zhang FC. Relationship between intestinal flora and inflammatory factors in patients with nonalcoholic steatohepatitis. Exper Ther Med 2018;15:723-726.

Zhang BX, Yue RS, Chen Y, Yang MY, Huang XY, Shui JC, et al. Gut microbiota, a potential new target for Chinese herbal medicines in treating diabetes mellitus. Evid-Based Complement Alternat Med 2019;2019:1-11.

Han GG, Lee JY, Jin GD, Park J, Choi YH, Chae BJ, et al. Evaluating the association between body weight and the intestinal microbiota of weaned piglets via 16S rRNA sequencing. Veter Microbiol 2016;196:55-62.

Opdahl LJ, Gonda MG, St-Pierre B. Identification of uncultured bacterial species from Firmicutes, Bacteroidetes and Candidatus saccharibacteria as candidate cellulose utilizers from the rumen of beef cows. Microorganisms 2018;6:17-29.

Sváb D, Falgenhauer L, Rohde M, Chakraborty T, Tóth I.Complete genome sequence of C130_2, a novel myovirus infecting pathogenic Escherichia coli and Shigella strains.Archives Virol 2019;164:321-324.

Wang Y, Wu YP, Wang YY, Xu H, Mei XQ, Yu DY, et al. Antioxidant properties of probiotic bacteria. Nutrients 2017;9:521-535.

Chang CW, Liu CY, Lee HC, Huang YH, Li LH, Chiau JC, et al. Lactobacillus casei variety rhamnosus probiotic preventively attenuates 5-fluorouracil/oxaliplatin-induced intestinal injury in a syngeneic colorectal cancer model.Front Microbiol 2018;9:983-997.

Guo S, Gillingham T, Guo Y, Meng D, Zhu W, Walker WA, et al. Secretions of bifiodobacterium infantis and lactobacillus acidophilus protect intestinal epithelial barrier function. J Pediatr Gastroenterol Nutr 2017;64:404-412.

Wang Y, Xu G, Wang J, Li XH, Sun P, Zhang W.Relationship of Th17/Treg cells and radiation pneumonia in locally advanced esophageal carcinoma. Anticancer Res 2017;37:4643-4647.

Klasen C, Meyer A, Wittekind PS, Waqué I, Nabhani S, Kofler DM. Prostaglandin receptor EP4 expression by Th17 cells is associated with high disease activity in ankylosing spondylitis. Arthritis Res Ther 2019; 21:159-171.

Tang M, Cheng L, Li F, Wu B, Chen P, Zhan Y, et al. Transcription factor IRF4 dysfunction affects the immunosuppressive function of Treg cells in patients with primary immune thrombocytopenia. Biomed Res Int 2019;2019:1-11.

Bin DK, Bartolucci S, d'Hennezel E, Piccirillo CA. Signaling through gp130 compromises suppressive function in human FOXP3+ regulatory T cells. Front Immunol 2019;10:1532-1544.

Artis D. Epithelial-cell recognition of commensal bacteria and maintenance of immune homeostasis in the gut. Nature Rev Immunol 2008;8:411-420.

D"Ettorre G, Rossi G, Scagnolari C, Andreotti M, Giustini N, Serafino S, et al. Probiotic supplementation promotes a reduction in T-cell activation, an increase in Th17 frequencies, and a recovery of intestinal epithelium integrity and mitochondrial morphology in ART-treated HIV-1-positive patients. Immun Inflam Dis 2017;5:244-260.

Xiao S, Li Q, Hu K, He Y, Ai Q, Hu L, et al. Vitamin A and retinoic acid exhibit protective effects on necrotizing enterocolitis by regulating intestinal flora and enhancing the intestinal epithelial barrier. Arch Med Res 2018;49:1-9.

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