<italic style="font-style: italic">Morinda citrifolia</italic> (Noni) Juice Suppresses A549 Human Lung Cancer Cells via Inhibiting AKT/Nuclear Factor-κB Signaling Pathway

Ling-di MA; Gui-bin LIN; Lu-bo YANG; Jia-lin CAO; Jian WANG; Qiong-di CHEN; Wei-qiang LI; Wang-jing ZHONG

doi:10.1007/s11655-020-3421-y

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Morinda citrifolia (Noni) Juice Suppresses A549 Human Lung Cancer Cells via Inhibiting AKT/Nuclear Factor-κB Signaling Pathway

Original Article | Updated：2021-09-01

- Morinda citrifolia (Noni) Juice Suppresses A549 Human Lung Cancer Cells via Inhibiting AKT/Nuclear Factor-κB Signaling Pathway
- Morinda citrifolia (Noni) Juice Suppresses A549 Human Lung Cancer Cells via Inhibiting AKT/Nuclear Factor-κB Signaling Pathway
- Chinese Journal of Integrative Medicine 2021年27卷第9期页码：688-695
- Affiliations：
  
  1.Laboratory Center, the Third People's Hospital of Huizhou, Affiliated Hospital of Guangzhou Medical University, Huizhou, Guangdong Province (516002), China
  2.Clinical Laboratory, the Third People's Hospital of Huizhou, Affiliated Hospital of Guangzhou Medical University, Huizhou, Guangdong Province (516002), China
- Author bio：
  
  Dr. MA Ling-di, E-mail: lingdimawsh@126.com
- Funds：
- DOI：10.1007/s11655-020-3421-y
  中图分类号：
- 纸质出版日期：2021-09-01，
  
  网络出版日期：2020-07-09，
  
  录用日期：2020-03-30
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Ling-di MA, Gui-bin LIN, Lu-bo YANG, 等. Morinda citrifolia (Noni) Juice Suppresses A549 Human Lung Cancer Cells via Inhibiting AKT/Nuclear Factor-κB Signaling Pathway[J]. Chinese Journal of Integrative Medicine, 2021,27(9):688-695.

Ling-di MA, Gui-bin LIN, Lu-bo YANG, et al. Morinda citrifolia (Noni) Juice Suppresses A549 Human Lung Cancer Cells via Inhibiting AKT/Nuclear Factor-κB Signaling Pathway[J]. Chinese Journal of Integrative Medicine, 2021,27(9):688-695.
Ling-di MA, Gui-bin LIN, Lu-bo YANG, 等. Morinda citrifolia (Noni) Juice Suppresses A549 Human Lung Cancer Cells via Inhibiting AKT/Nuclear Factor-κB Signaling Pathway[J]. Chinese Journal of Integrative Medicine, 2021,27(9):688-695. DOI： 10.1007/s11655-020-3421-y.

Ling-di MA, Gui-bin LIN, Lu-bo YANG, et al. Morinda citrifolia (Noni) Juice Suppresses A549 Human Lung Cancer Cells via Inhibiting AKT/Nuclear Factor-κB Signaling Pathway[J]. Chinese Journal of Integrative Medicine, 2021,27(9):688-695. DOI： 10.1007/s11655-020-3421-y.

摘要

Abstract

Objective:

To study the mechanism of the anti-tumor effect of

Morinda citrifolia

(noni).

Methods:

The influences of noni juice on cell proliferation

apoptosis

invasion

migration and the activity of AKT/nuclear factor-κB (NF-κB) signaling pathway in A549 human lung cancer cells were detected by MTT

cell counting kit-8

colony formation

Annexin V/PI double labeling

transwell

scratch test and immunoblotting assay

respectively. A549 cells were inoculated into the right axilla of nude mice

followed by noni juice treatment. The body weight of the nude mice was weighed

and the tumor volume and weight were measured. Cell proliferation and expression of apoptosis-related proteins were measured by immunohistochemistry

and the activity of NF-κB signaling pathway was measured by immunoblotting.

Results:

The

in vitro

studies showed that noni juice inhibited the A549 cells proliferation

migration and invasion. Noni juice also promoted cells apoptosis in A549 cells. Immunoblotting assay showed that the phosphorylation level of AKT

p50

and STAT3 proteins was inhibited to different extents after noni juice treatment. The

in vivo

studies showed that noni juice effectively suppressed tumor formation of A549 cells in nude mice. Noni juice treatment inhibited the expression of Ki67

PCNA

and Bcl-2 protein in the tumor; while promoted the expression of caspase-3 protein. Additionally

we also found that noni juice treatment could restrain the activity of AKT/NF-κB signaling pathway in the tumor tissue.

Conclusion:

Noni juice inhibited the proliferation of A549 lung cancer cells

induced apoptosis

and inhibited cell invasion and migration via regulating AKT/NF-κB signaling pathway.

关键词

Keywords

Morinda citrifolianoni juiceChinese medicinelung cancer cellAKT/NF-κB signaling pathway

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Morinda citrifolia (Noni) Juice Suppresses A549 Human Lung Cancer Cells via Inhibiting AKT/Nuclear Factor-κB Signaling Pathway

Morinda citrifolia (Noni) Juice Suppresses A549 Human Lung Cancer Cells via Inhibiting AKT/Nuclear Factor-κB Signaling Pathway

DOI：10.1007/s11655-020-3421-y

摘要

Abstract

关键词

Keywords

references