手针镇痛的穴位分子启动机制——NFκB信号通路

Kuo ZHANG; Xue ZHAO; Tao YANG; Yan-fang ZHANG; Ya-wen YAN; Zhi-fang XU; Yi-nan GONG; Yang-yang LIU; Yong-ming GUO; Yi GUO

doi:10.1007/s11655-020-3435-6

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手针镇痛的穴位分子启动机制——NFκB信号通路

Acupuncture Research | Updated：2021-11-29

- 手针镇痛的穴位分子启动机制——NFκB信号通路
- Initiation of Acupoint Molecular Mechanisms for Manual Acupuncture Analgesia—Nuclear Factor κB Signaling Pathway^*
- Chinese Journal of Integrative Medicine 2021年27卷第12期页码：933-939
- Affiliations：
  
  1.Academy of Medical Engineering and Translational Medicine,Tianjin University, Tianjin (300072), China
  2.Research Center of Experimental Acupuncture Science, Tianjin University of Traditional Chinese Medicine, Tianjin (301617), China
  3.Affiliated Hospital of Municipal Institute of Traditional Chinese Medicine of Changzhi City, Shanxi Province (046000), China
  4.Sanhuai Road Community Health Service Center, Tanggu Street, Binhai New Area, Tianjin (300451), China
  5.School of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin (301617), China
- Author bio：
  
  Dr. ZHAO Xue, E-mail: zhaoxue0216@126.com
- Funds：
  
  the China Postdoctoral Science Foundation(2019M651044);the National Natural Science Foundation of China(81330088)
- DOI：10.1007/s11655-020-3435-6
  中图分类号：
- 纸质出版日期：2021-11-15，
  
  网络出版日期：2020-11-02，
  
  录用日期：2020-06-01
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Kuo ZHANG, Xue ZHAO, Tao YANG, 等. 手针镇痛的穴位分子启动机制——NFκB信号通路[J]. Chinese Journal of Integrative Medicine, 2021,27(12):933-939.

Kuo ZHANG, Xue ZHAO, Tao YANG, et al. Initiation of Acupoint Molecular Mechanisms for Manual Acupuncture Analgesia—Nuclear Factor κB Signaling Pathway^*[J]. Chinese Journal of Integrative Medicine, 2021,27(12):933-939.
Kuo ZHANG, Xue ZHAO, Tao YANG, 等. 手针镇痛的穴位分子启动机制——NFκB信号通路[J]. Chinese Journal of Integrative Medicine, 2021,27(12):933-939. DOI： 10.1007/s11655-020-3435-6.

Kuo ZHANG, Xue ZHAO, Tao YANG, et al. Initiation of Acupoint Molecular Mechanisms for Manual Acupuncture Analgesia—Nuclear Factor κB Signaling Pathway^*[J]. Chinese Journal of Integrative Medicine, 2021,27(12):933-939. DOI： 10.1007/s11655-020-3435-6.

摘要

目的:

确定穴位局部的关键信号分子并探讨其在手针镇痛中的作用.

方法:

进行三步研究. 实验1

将12只Wistar大鼠随机分为对照组、对照+手针1组和对照+手针7组

对足三里穴区组织进行全基因组分析. 实验2

将大鼠分为生理盐水组、生理盐水+手针组和CFA+手针组

每组6只

进行足底PWL检测

并对足三里穴区组织中的NFκB p-p65、NFκB p-p50进行免疫组化和Western blot检测. 实验3

将24只大鼠分为生理盐水+DMSO组、CFA+DMSO组、CFA+DMSO+手针组和CFA+BAY 11-7082+手针组

进行足底PWL检测

并对足三里穴区组织中的NFκB p65进行免疫荧光检测.

结果:

(1) 在对照+手针7组中

Rela、Nfkb1、Nfkbia、Il1b、Il6、Cxcl1、Ccl2基因表达显著增加 (

0.05或

0.01). (2) 与CFA组相比

从针刺第1天至第7天

CFA+手针组PWL均显著提高 (

0.01). 与对照组和CFA组相比

Western blot结果与免疫组织化学一致

手针相关组的NFκB p-p65和NFκB p-p50表达显著增加 (

0.05). (3) 与CFA+DMSO+手针组相比

CFA+BAY 11-7082+手针组的PWL从第3天起显著下降

并持续到第5天和第7天 (分别为

0.05和

0.01)

且NFκB p65的表达也显著降低 (

0.01).

结论:

手针引起的穴位局部NFκB信号级联反应是其镇痛效应启动的重要步骤

为手针镇痛效应的启动提供了新的证据

提高了对针刺镇痛科学基础的认识

Abstract

Objective:

To identify the prominent molecular signaling in acupoints and explore their roles in initiating the analgesia effect of manual acupuncture (MA).

Methods:

A three-step study was conducted

the experiment 1 was a genome-wide analysis of the tissue at acupoint Zusanli (ST 36)

including 12 Wistar rats which were divided into control

control+MA1

and control+MA7 groups. In the experiment 2

the paw withdrawal latency (PWL)

immunohistochemistry and Western blot analysis of phospho-nuclear factor kappa B (NFκB) p65 (p-p65)

phospho-NFκB p50 (p-p50) at ST 36 were performed on rats of saline

saline+MA

and complete Freund's adjuvant (CFA) +MA groups (

=6). In experiment 3

24 rats were divided into saline+DMSO

CFA+DMSO

CFA+DMSO+MA

and CFA+BAY 11-7082+MA groups

the PWL and immunofluorescence assay of NFκB p65 at ST 36 was conducted.

Results:

(1) The gene: inhibitor of NFκB (Nfkbia)

interleukin-1β (Il1b)

interleukin-6 (Il6)

chemokine c-x-c motif ligand 1 (Cxcl1)

monocyte chemoattractant protein-1 (MCP-1/Ccl2) expressions in the control+MA7 group were significantly increased (

0.05 or

0.01)

and the expression of NFκB p65 (Rela)

NFκB p50 (Nfkb1) were increased in the control+MA7 group (

0.05). (2) CFA+MA groups showed increased PWL from day 1 to 7 (

0.01 vs. CFA)

and the Western blot results were consistent with immunohistochemistry

the expression of NFκB p-p65 and NFκB p-p50 were significantly increased in the MA-related groups compared with control and CFA groups (

0.05). (3) Compared with the CFA+DMSO+MA group

the PWL of the CFA+ BAY 11-7082+MA group decreased significantly and continued until day 5 and 7 (

0.05 and

0.01

respectively)

and the NFκB p65 expression of CFA+BAY 11-7082+MA was significantly reduced compared with CFA+DMSO+MA (

0.01).

Conclusion:

Local NFκB signaling cascade in acupoint caused by MA is an important step in initiating the analgesic effect

which would provide new evidence for the initiation of MA-effect and improve the understanding of the scientific basis of acupuncture analgesia.

关键词

Keywords

manual acupunctureacupointanalgesianuclear factor kappa B

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