Ginsenoside Rb1 Protects Human Umbilical Vein Endothelial Cells against High Glucose-Induced Mitochondria-Related Apoptosis through Activating SIRT3 Signalling Pathway*

Shi-ye KE; Shu-jie YU; Ding-hui LIU; Guang-yao SHI; Min WANG; Bin ZHOU; Lin WU; Zhi-ming SONG; Jie-ming ZHU; Chao-dong WU; Xiao-xian QIAN

doi:10.1007/s11655-020-3478-8

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Ginsenoside Rb1 Protects Human Umbilical Vein Endothelial Cells against High Glucose-Induced Mitochondria-Related Apoptosis through Activating SIRT3 Signalling Pathway*

Original Article | Updated：2021-09-01

- Ginsenoside Rb1 Protects Human Umbilical Vein Endothelial Cells against High Glucose-Induced Mitochondria-Related Apoptosis through Activating SIRT3 Signalling Pathway*
- Ginsenoside Rb1 Protects Human Umbilical Vein Endothelial Cells against High Glucose-Induced Mitochondria-Related Apoptosis through Activating SIRT3 Signalling Pathway*
- Chinese Journal of Integrative Medicine 2021年27卷第5期页码：336-344
- Affiliations：
  
  1.Department of Cardiology, the Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou (510630), China
  2.Department of Cardiology, the Eighth Affiliated Hospital of Sun Yat-Sen University, Shenzhen, Guangdong Province (518033), China
  3.Department of Cardiology, the First Affiliated Hospital of Henan University, Kaifeng, Henan Province (475001), China
  4.Department of Nutrition and Food Science, Texas A&M University, College Station, TX (77843), United States of America
  5.Institute of Integrated Traditional Chinese and Western Medicine, Sun Yat-Sen University, Guangzhou (510630), China
- Author bio：
  
  Prof. QIAN Xiao-xian, E-mail: qianxx@mail.sysu.edu.cn
- Funds：
  
  the National Natural Science Foundation of China(81370447);Science and Technology Planning Project of Guangdong Province, China(2016A050502014);the Ph.D. Start-up Fund of Natural Science Foundation of Guangdong Province, China(2015A030310048;2016A030310203)
- DOI：10.1007/s11655-020-3478-8
  中图分类号：
- 纸质出版日期：2021-05-01，
  
  网络出版日期：2020-01-09，
  
  录用日期：2019-08-21
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Shi-ye KE, Shu-jie YU, Ding-hui LIU, 等. Ginsenoside Rb1 Protects Human Umbilical Vein Endothelial Cells against High Glucose-Induced Mitochondria-Related Apoptosis through Activating SIRT3 Signalling Pathway*[J]. Chinese Journal of Integrative Medicine, 2021,27(5):336-344.

Shi-ye KE, Shu-jie YU, Ding-hui LIU, et al. Ginsenoside Rb1 Protects Human Umbilical Vein Endothelial Cells against High Glucose-Induced Mitochondria-Related Apoptosis through Activating SIRT3 Signalling Pathway*[J]. Chinese Journal of Integrative Medicine, 2021,27(5):336-344.
Shi-ye KE, Shu-jie YU, Ding-hui LIU, 等. Ginsenoside Rb1 Protects Human Umbilical Vein Endothelial Cells against High Glucose-Induced Mitochondria-Related Apoptosis through Activating SIRT3 Signalling Pathway*[J]. Chinese Journal of Integrative Medicine, 2021,27(5):336-344. DOI： 10.1007/s11655-020-3478-8.

Shi-ye KE, Shu-jie YU, Ding-hui LIU, et al. Ginsenoside Rb1 Protects Human Umbilical Vein Endothelial Cells against High Glucose-Induced Mitochondria-Related Apoptosis through Activating SIRT3 Signalling Pathway*[J]. Chinese Journal of Integrative Medicine, 2021,27(5):336-344. DOI： 10.1007/s11655-020-3478-8.

摘要

Abstract

Objective:

To investigate whether ginsenoside Rb1 (Rb1) can protect human umbilical vein endothelial cells (HUVECs) against high glucose-induced apoptosis and examine the underlying mechanism.

Methods:

HUVECs were divided into 5 groups: control group (5.5 mmol/L glucose)

high glucose (HG

40 mmol/L) treatment group

Rb1 (50 μmol/L) treatment group

Rb1 plus HG treatment group

and Rb1 and 3-(

H-1

3-triazol-4-yl) pyridine (3-TYP

16 μmol/L) plus HG treatment group. Cell viability was evaluated by cell counting kit-8 assay. Mitochondrial and intracellular reactive oxygen species were detected by MitoSox Red mitochondrial superoxide indicator and dichloro-dihydro-fluorescein diacetate assay

respectively. Annexin V/propidium iodide staining and fluorescent dye staining were used to measure the apoptosis and the mitochondrial membrane potential of HUVECs

respectively. The protein expressions of apoptosis-related proteins [Bcl-2

Bax

cleaved caspase-3 and cytochrome c (Cyt-c)]

mitochondrial biogenesis-related proteins [proliferator-activated receptor gamma coactivator 1-alpha

nuclear respiratory factor-1 and mitochondrial transcription factor A)]

acetylation levels of forkhead box O3a and SOD2

and sirtuin-3 (SIRT3) signalling pathway were measured by immunoblotting and immunoprecipitation.

Results:

Rb1 ameliorated survival in cells in which apoptosis was induced by high glucose (

0.05 or

0.01). Upon the addition of Rb1

mitochondrial and intracellular reactive oxygen species generation and malondialdehyde levels were decreased (

0.01)

while the activities of antioxidant enzymes were increased (

0.05 or

0.01). Rb1 preserved the mitochondrial membrane potential and reduced the release of Cyt-c from the mitochondria into the cytosol (

0.01). In addition

Rb1 upregulated mitochondrial biogenesis-associated proteins (

0.01). Notably

the cytoprotective effects of Rb1 were correlated with SIRT3 signalling pathway activation (

0.01). The effect of Rb1 against high glucose-induced mitochondria-related apoptosis was restrained by 3-TYP (

0.05 or

0.01).

Conclusion:

Rb1 could protect HUVECs from high glucose-induced apoptosis by promoting mitochondrial function and suppressing oxidative stress through the SIRT3 signalling pathway.

关键词

Keywords

ginsenoside Rb1high glucosehuman umbilical vein endothelial cellsapoptosismitochondriaSIRT3 signalling pathway

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