Effect of Kangquan Recipe (康泉方) on BAMBI Expression in Hypothalamic-Pituitary-Prostate in Rats with Benign Prostatic Hyperplasia*

Wen-fan CHEN; Zong-bao YANG; A-xiang PENG; Xiao-qing HUANG; Hui SU; Ting-ting CHEN; Yuan-peng HUANG

doi:10.1007/s11655-020-3481-0

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Effect of Kangquan Recipe (康泉方) on BAMBI Expression in Hypothalamic-Pituitary-Prostate in Rats with Benign Prostatic Hyperplasia*

Original Article | Updated：2021-09-01

- Effect of Kangquan Recipe (康泉方) on BAMBI Expression in Hypothalamic-Pituitary-Prostate in Rats with Benign Prostatic Hyperplasia*
- Effect of Kangquan Recipe (康泉方) on BAMBI Expression in Hypothalamic-Pituitary-Prostate in Rats with Benign Prostatic Hyperplasia*
- Chinese Journal of Integrative Medicine 2021年27卷第5期页码：361-368
- Affiliations：
  
  1.Department of Traditional Chinese Medicine, Zhongshan Hospital, Xiamen University, Xiamen, Fujian Province (361001), China
  2.Department of Traditional Chinese Medicine, School of Medicine, Xiamen University, Xiamen, Fujian Province (361101), China
  3.Department of Internal Traditional Chinese Medicine, Xiamen Hospital of Traditional Chinese Medicine, Xiamen, Fujian Province (361009), China
- Author bio：
  
  Prof. HUANG Yuan-peng, E-mail:huangyp998@sina.com
- Funds：
  
  the National Natural Science Foundation of China(81674041);the Foundation of Science and Technology Department of Xiamen(3502Z20164013);the Natural Science Foundation of Fujian Province(2018J01395)
- DOI：10.1007/s11655-020-3481-0
  中图分类号：
- 纸质出版日期：2021-05-01，
  
  网络出版日期：2020-09-25，
  
  录用日期：2018-12-26
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Wen-fan CHEN, Zong-bao YANG, A-xiang PENG, 等. Effect of Kangquan Recipe (康泉方) on BAMBI Expression in Hypothalamic-Pituitary-Prostate in Rats with Benign Prostatic Hyperplasia*[J]. Chinese Journal of Integrative Medicine, 2021,27(5):361-368.

Wen-fan CHEN, Zong-bao YANG, A-xiang PENG, et al. Effect of Kangquan Recipe (康泉方) on BAMBI Expression in Hypothalamic-Pituitary-Prostate in Rats with Benign Prostatic Hyperplasia*[J]. Chinese Journal of Integrative Medicine, 2021,27(5):361-368.
Wen-fan CHEN, Zong-bao YANG, A-xiang PENG, 等. Effect of Kangquan Recipe (康泉方) on BAMBI Expression in Hypothalamic-Pituitary-Prostate in Rats with Benign Prostatic Hyperplasia*[J]. Chinese Journal of Integrative Medicine, 2021,27(5):361-368. DOI： 10.1007/s11655-020-3481-0.

Wen-fan CHEN, Zong-bao YANG, A-xiang PENG, et al. Effect of Kangquan Recipe (康泉方) on BAMBI Expression in Hypothalamic-Pituitary-Prostate in Rats with Benign Prostatic Hyperplasia*[J]. Chinese Journal of Integrative Medicine, 2021,27(5):361-368. DOI： 10.1007/s11655-020-3481-0.

摘要

Abstract

Objective:

To investigate the effect of Kangquan Recipe (康泉方

KQR) on bone morphogenetic protein and activin membrane-bound inhibitor (BAMBI) expression and its mechanism in rats with benign prostatic hyperplasia (BPH).

Methods:

Forty-eight male Sprague-Dawley rats were divided into 6 groups using a random number table

with 8 in each group: the normal group (normal saline 10 mL/kg)

the model group (normal saline 10 mL/kg)

the finasteride group (0.5 mg/kg)

the low-dose KQR group (3.5 g/kg)

the middle-dose KQR group (7 g/kg)

and the high-dose KQR group (14 g/kg). The 40 rats were subcutaneously injected with testosterone propionate after castration for 30 days to establish the BPH rat model except for those in the normal group. At the same time

the corresponding drugs were administered by gavage for 30 consecutive days. The effects of different doses of KQR on the protate wet weight

prostate volume and prostate index (PI) were observed. The changes in histopathology were monitored with hematoxylin-eosin staining. BAMBI protein and mRNA expression contents were determined by Western blot and quantitative real-time polymerase chain reaction

respectively.

Results:

All doses of KQR could decrease prostatic epithelial tissue proliferation. Compared to the model group

the high and middle-dose KQR significantly reduced prostate wet weight

prostate volume and PI; increased BAMBI protein expression in the hypothalamus

pituitary and prostate tissue; all doses of KQR up-regulated BAMBI mRNA expression in serum

prostatic fluid and prostate tissue (

0.05 or

0.01).

Conclusions:

KQR could inhibit the proliferation of rat prostatic tissue

promote BAMBI protein expression in the hypothalamic-pituitary-prostate of rats with BPH; and increase BAMBI mRNA expression in the blood

prostatic fluid and prostate tissue of rats with BPH

showing a dose-effect relationship. KQR can be used as a potential drug for the treatment of BPH.

关键词

Keywords

Kangquan Recipebenign prostatic hyperplasiahypothalamic-pituitary-prostateBAMBIChinese medicine

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