Effects of Shenfu Injection (参附注射液) on Inflammatory Response during Post-Resuscitation Myocardial Dysfunction after Cardiac Arrest in Swine

Wei GU; Xiao-min HOU; Chun-sheng LI

doi:10.1007/s11655-021-2855-2

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Effects of Shenfu Injection (参附注射液) on Inflammatory Response during Post-Resuscitation Myocardial Dysfunction after Cardiac Arrest in Swine

Original Article | Updated：2021-09-01

- Effects of Shenfu Injection (参附注射液) on Inflammatory Response during Post-Resuscitation Myocardial Dysfunction after Cardiac Arrest in Swine
- Effects of Shenfu Injection (参附注射液) on Inflammatory Response during Post-Resuscitation Myocardial Dysfunction after Cardiac Arrest in Swine
- Chinese Journal of Integrative Medicine 2021年27卷第6期页码：417-423
- Affiliations：
  
  1.Department of Emergency Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing Key Laboratory of Cardiopulmonary Cerebral Resuscitation, Beijing (100050), China
  2.Department of Emergency Medicine, Beijing Chaoyang Hospital, Capital Medical University, Beijing Key Laboratory of Cardiopulmonary Cerebral Resuscitation, Beijing (100020), China
- Author bio：
  
  Prof. LI Chun-sheng, E-mail: lcscyyy@163.com
- Funds：
  
  the Beijing Natural Science Foundation(7182055);Beijing Municipal Administration of Hospitals Incubating Program(2016022)
- DOI：10.1007/s11655-021-2855-2
  中图分类号：
- 纸质出版日期：2021-06-01，
  
  网络出版日期：2021-01-09，
  
  录用日期：2018-05-29
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Wei GU, Xiao-min HOU, Chun-sheng LI. Effects of Shenfu Injection (参附注射液) on Inflammatory Response during Post-Resuscitation Myocardial Dysfunction after Cardiac Arrest in Swine[J]. Chinese Journal of Integrative Medicine, 2021,27(6):417-423.

Wei GU, Xiao-min HOU, Chun-sheng LI. Effects of Shenfu Injection (参附注射液) on Inflammatory Response during Post-Resuscitation Myocardial Dysfunction after Cardiac Arrest in Swine[J]. Chinese Journal of Integrative Medicine, 2021,27(6):417-423.
Wei GU, Xiao-min HOU, Chun-sheng LI. Effects of Shenfu Injection (参附注射液) on Inflammatory Response during Post-Resuscitation Myocardial Dysfunction after Cardiac Arrest in Swine[J]. Chinese Journal of Integrative Medicine, 2021,27(6):417-423. DOI： 10.1007/s11655-021-2855-2.

Wei GU, Xiao-min HOU, Chun-sheng LI. Effects of Shenfu Injection (参附注射液) on Inflammatory Response during Post-Resuscitation Myocardial Dysfunction after Cardiac Arrest in Swine[J]. Chinese Journal of Integrative Medicine, 2021,27(6):417-423. DOI： 10.1007/s11655-021-2855-2.

摘要

Abstract

Objective:

To investigate whether Shenfu Injection (SFI

参附注射液) can alleviate post-resuscitation myocardial dysfunction by inhibiting the inflammatory response.

Methods:

After 8 min of ventricular fibrillation and 2 min of basic life support

24 pigs were randomly divided into 3 groups (n=8)

which were given intravenous bolus injections of SFI (1.0 mL/kg)

epinephrine (EP

0.02 mg/kg) and normal saline (SA)

respectively. The animals were sacrificed at 24 h after restoration of spontaneous circulation (ROSC)

and serum interleuking-6 (IL-6) and tumor necrosis factor-α (TNF-α) levels were measured by enzyme-linked immunosorbent assay (ELISA); expressions of Toll-like receptor 4 (TLR4)/nuclear factor kappa B (NF-κB) mRNAs and proteins were determined by RT-PCR and Western blot

respectively.

Results:

Compared with the EP and the SA groups

the ultrastructure of myocardial cells were slightly damaged and the systolic function of the left ventricle was markedly improved in the SFI group at 24 h after ROSC (

0.05). In addition

compared with the EP and SA groups

the SFI group also showed significantly reduced levels of serum IL-6 and TNF-α

protein and mRNA levels of myocardial NF-κB and TLR4 (

0.05).

Conclusions:

Activation of TLR4/NF-κB signaling pathway may be involved in the pathological mechanisms of post-resuscitation myocardial dysfunction. SFI may block NF-κB-mediated inflammatory response by reducing the activity of NF-κB and the level of TNF-α

thus playing a protective role in post-resuscitation myocardial dysfunction.

关键词

Keywords

Shenfu InjectionChinese medicinecardiopulmonary resuscitationpost-resuscitation myocardial dysfunctionTLR4/NF-κB

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