Wide Pulse Width Electroacupuncture Ameliorates Denervation-Induced Skeletal Muscle Atrophy in Rats via IGF-1/PI3K/Akt Pathway*

Xiao-qing HUANG; Jin-sen XU; Xiao-ran YE; Xuan CHEN

doi:10.1007/s11655-021-2865-0

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Wide Pulse Width Electroacupuncture Ameliorates Denervation-Induced Skeletal Muscle Atrophy in Rats via IGF-1/PI3K/Akt Pathway*

Acupuncture Research | Updated：2021-09-01

- Wide Pulse Width Electroacupuncture Ameliorates Denervation-Induced Skeletal Muscle Atrophy in Rats via IGF-1/PI3K/Akt Pathway*
- Wide Pulse Width Electroacupuncture Ameliorates Denervation-Induced Skeletal Muscle Atrophy in Rats via IGF-1/PI3K/Akt Pathway*
- Chinese Journal of Integrative Medicine 2021年27卷第6期页码：446-454
- Affiliations：
  
  1.Key Laboratory of Acupuncture Instrument, Fujian Academy of Chinese Medical Sciences, Fuzhou (350003), China
  2.Fujian Key Laboratory of Propagated Sensation along Meridian, Institute of Meridians and Collaterals Medicine, Fujian Academy of Chinese Medical Sciences, Fuzhou (350003), China
- Author bio：
  
  Associate Prof. CHEN Xuan, E-mail:chenxuanfz@aliyun.com
- Funds：
  
  the National Natural Science Foundation of China(81503657;81373733);the Basic Research Project of Public Welfare Research Institutes in Fujian Province(2016R1033-6)
- DOI：10.1007/s11655-021-2865-0
  中图分类号：
- 纸质出版日期：2021-06-01，
  
  网络出版日期：2021-03-04，
  
  录用日期：2020-01-15
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Xiao-qing HUANG, Jin-sen XU, Xiao-ran YE, 等. Wide Pulse Width Electroacupuncture Ameliorates Denervation-Induced Skeletal Muscle Atrophy in Rats via IGF-1/PI3K/Akt Pathway*[J]. Chinese Journal of Integrative Medicine, 2021,27(6):446-454.

Xiao-qing HUANG, Jin-sen XU, Xiao-ran YE, et al. Wide Pulse Width Electroacupuncture Ameliorates Denervation-Induced Skeletal Muscle Atrophy in Rats via IGF-1/PI3K/Akt Pathway*[J]. Chinese Journal of Integrative Medicine, 2021,27(6):446-454.
Xiao-qing HUANG, Jin-sen XU, Xiao-ran YE, 等. Wide Pulse Width Electroacupuncture Ameliorates Denervation-Induced Skeletal Muscle Atrophy in Rats via IGF-1/PI3K/Akt Pathway*[J]. Chinese Journal of Integrative Medicine, 2021,27(6):446-454. DOI： 10.1007/s11655-021-2865-0.

Xiao-qing HUANG, Jin-sen XU, Xiao-ran YE, et al. Wide Pulse Width Electroacupuncture Ameliorates Denervation-Induced Skeletal Muscle Atrophy in Rats via IGF-1/PI3K/Akt Pathway*[J]. Chinese Journal of Integrative Medicine, 2021,27(6):446-454. DOI： 10.1007/s11655-021-2865-0.

摘要

Abstract

Objective:

To evaluate the effect of the pulse width of electroacupuncture (EA) in the treatment of denervation-induced skeletal muscle atrophy in rats and examine the role of insulin-like growth factor 1 (IGF-1)/phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway during EA.

Methods:

Sciatic nerve functional index (SFI)

muscle wet weight and the cross-sectional area (CSA) of the gastrocnemius muscle were analyzed after treatment in model rats with EA of various pulse widths (0.5

100 and 200 ms). The apoptosis index (AI) and paired box (PAX) 3 and PAX7 protein expression were also determined. Further

the mRNA and protein expressions of components of IGF-1/PI3K/Akt pathway and their downstream targets were determined

along with the inhibiting effect of the pathway with a PI3-specific inhibitor.

Results:

EA with a pulse width of 200 ms was found to have the best effect with regard to increasing SFI

CSA and muscle weight

decreasing AI

and increasing the expression of PAX3 and PAX7. The IGF-1/PI3K/Akt pathway was found to be activated by denervation

although the downstream forkhead box O (FoxO) pathway was not suppressed by its activation. The PI3K/Akt pathway and its downstream molecule mammalian target of rapamycin (mTOR) were up-regulated further by EA to promote muscle protein synthesis. Meanwhile

the expressions of downstream FoxO and F-box protein 32 (ATROGIN-1) were down-regulated to reduce protein degradation.

Conclusions:

EA with 200-ms pulse width was found to have a more significant effect than 0.5-ms EA. The positive effects of EA disappeared after inhibition of the PI3K/Akt pathway.

关键词

Keywords

denervationmuscleatrophyelectroacupuncturepulse widthIGF-1/PI3K/Akt pathway

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