参附注射液对失血性休克猪血管内皮损伤的保护作用

Ming-qing ZHANG; Qiang ZHANG; Wei YUAN; Jun-yuan WU; Yong LIANG; Hong-jie QIN; Chun-sheng LI

doi:10.1007/s11655-021-2876-x

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参附注射液对失血性休克猪血管内皮损伤的保护作用

Original Article | Updated：2022-09-08

- 参附注射液对失血性休克猪血管内皮损伤的保护作用
- Protective Effect of Shenfu Injection on Vascular Endothelial Damage in a Porcine Model of Hemorrhagic Shock
- Chinese Journal of Integrative Medicine 2022年28卷第9期页码：794-801
- Affiliations：
  
  1.Department of Emergency Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing (100050), China
  2.Department of Critical Care Medicine, Peking University Third Hospital, Beijing (100191), China
  3.Department of Emergency Medicine, Beijing Chaoyang Hospital, Capital Medical University,Beijing (100020), China
  4.Department of Emergency Medicine, Beijing Luhe Hospital, Capital Medical University, Beijing(101199), China
- Author bio：
  
  Prof. LI Chun-sheng, E-mail: lcscyyy@163.com
- Funds：
  
  the National Natural Science Foundation of China(81372025);the 2015 Annual Special Cultivation and Development Project for the Technology Innovation of Beijing(Z151100001615056)
- DOI：10.1007/s11655-021-2876-x
  中图分类号：
- 纸质出版日期：2022-09，
  
  网络出版日期：2022-01-13，
  
  录用日期：2021-03-30
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Ming-qing ZHANG, Qiang ZHANG, Wei YUAN, 等. 参附注射液对失血性休克猪血管内皮损伤的保护作用[J]. Chinese Journal of Integrative Medicine, 2022,28(9):794-801.

Ming-qing ZHANG, Qiang ZHANG, Wei YUAN, et al. Protective Effect of Shenfu Injection on Vascular Endothelial Damage in a Porcine Model of Hemorrhagic Shock[J]. Chinese Journal of Integrative Medicine, 2022,28(9):794-801.
Ming-qing ZHANG, Qiang ZHANG, Wei YUAN, 等. 参附注射液对失血性休克猪血管内皮损伤的保护作用[J]. Chinese Journal of Integrative Medicine, 2022,28(9):794-801. DOI： 10.1007/s11655-021-2876-x.

Ming-qing ZHANG, Qiang ZHANG, Wei YUAN, et al. Protective Effect of Shenfu Injection on Vascular Endothelial Damage in a Porcine Model of Hemorrhagic Shock[J]. Chinese Journal of Integrative Medicine, 2022,28(9):794-801. DOI： 10.1007/s11655-021-2876-x.

摘要

目的:

探讨参附注射液对猪失血性休克 (HS) 模型内皮损伤的影响.

方法:

32头实验猪采用快速放血法维持在平均动脉压为40±3毫米汞柱并保持60分钟后

分别随机分为静脉输血 (输血组)、输血联合生理盐水 (生理盐水组)、输血联合静脉输注参附注射液 (参附组) 或不复苏 (假手术组) . 在基线和HS后0、1、2、4和6小时采集静脉血样本并进行分析. 通过酶联免疫吸附试验 (ELISA) 测定肿瘤坏死因子-α (TNF-α)、血清白介素-6和白介素-10的水平; Western blot检测血管细胞粘附分子-1 (VCAM-1)、细胞间粘附分子-1 (ICAM-1)、血管性血友病因子 (vWF)、纤溶酶原激活物抑制剂-1 (PAI-1)、Bcl-2、Bax和caspase-3蛋白的表达.

结果:

参附组在复苏的整个6小时内

实验猪血清TNF-α水平显著低于其他组

IL-6水平在4和6小时低于其他组 (

0.01或

0.05) . 复苏全程参附组血清IL-10浓度显著升高其他组

差异有统计学意义 (

0.01) . 血管组织的Western blot和免疫组化显示

与其他组相比

参附组caspase-3的表达下调

Bcl-2和Bax的表达上调 (

0.05) .

结论:

参附注射液通过抑制细胞凋亡、抑制促炎细胞因子的形成及减少内皮细胞的活化

减轻了失血性休克猪模型中的内皮损伤.

Abstract

Objective:

To investigate the effects of Shenfu Injection (SFI) on endothelial damage in a porcine model of hemorrhagic shock (HS).

Methods:

After being bled to a mean arterial pressure of 40±3 mm Hg and held for 60 min

32 pigs were treated with a venous injection of either shed blood (transfusion group)

shed blood and saline (saline group)

shed blood and SFI (SFI group) or without resuscitation (sham group). Venous blood samples were collected and analyzed at baseline and 0

and 6 h after HS. Tumor necrosis factor-α (TNF-α)

serum interleuking (IL)-6

and IL-10 levels were measured by enzyme-linked immunosorbent assay (ELISA); expressions of vascular cell adhesion molecule-1 (VCAM-1)

intercellular adhesion molecule 1 (ICAM -1)

von Willebrand factor (vWF)

plasminogen activator inhibitor-1 (PAI-1) and Bcl-2

Bax

and caspase-3 proteins were determined by Western blot.

Results:

The serum level of TNF-α in the SFI group was significantly lower than in the other groups at 0

and 2 h after HS

while the level of IL-6 was lower at 4 and 6 h compared with the saline group (

0.01 or

0.05). The concentration of serum IL-10 was significantly higher in the SFI group than in the other groups at 0

and 6 h after HS (

0.01). Western blot and immunohistochemistry of vascular tissue showed that the expression of caspase-3 was downregulated

and that of Bcl-2 and Bax was upregulated in the SFI group compared to other groups (

0.05).

Conclusion:

SFI attenuated endothelial injury in the porcine model of HS by inhibiting cell apoptosis

suppressing the formation of proinflammatory cytokines

and reducing endothelial activation.

关键词

失血性休克参附注射液血管内皮损伤中药

Keywords

hemorrhagic shockShenfu Injectionvascular endothelial damageChinese medicine

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