Jiaotai Pill (交泰丸) Alleviates Insomnia through Regulating Monoamine and Organic Cation Transporters in Rats

Zhi-hui LI; Peng-kai MA; Yun-fang HUANG; Zhe ZHANG; Wei ZHENG; Jian-hua CHEN; Chang-e GUO; Ning CHEN; Xin-ning BI; Yu-jie ZHANG

doi:10.1007/s11655-021-3284-y

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Jiaotai Pill (交泰丸) Alleviates Insomnia through Regulating Monoamine and Organic Cation Transporters in Rats

Original Article | Updated：2021-08-16

- Jiaotai Pill (交泰丸) Alleviates Insomnia through Regulating Monoamine and Organic Cation Transporters in Rats
- Jiaotai Pill (交泰丸) Alleviates Insomnia through Regulating Monoamine and Organic Cation Transporters in Rats
- Chinese Journal of Integrative Medicine 2021年27卷第3期页码：183-191
- Affiliations：
  
  School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing (102488), China
- Author bio：
  
  Correspondence to: Prof. ZHANG Yu-jie, E-mail: zhyj227@126.com
- Funds：
  
  the National Natural Science Foundation of China
- DOI：10.1007/s11655-021-3284-y
  中图分类号：
- 纸质出版日期：2021-03-01，
  
  网络出版日期：2021-01-9，
  
  录用日期：2020-06-21
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Zhi-hui LI, Peng-kai MA, Yun-fang HUANG, 等. Jiaotai Pill (交泰丸) Alleviates Insomnia through Regulating Monoamine and Organic Cation Transporters in Rats[J]. Chinese Journal of Integrative Medicine, 2021,27(3):183-191.

Zhi-hui LI, Peng-kai MA, Yun-fang HUANG, et al. Jiaotai Pill (交泰丸) Alleviates Insomnia through Regulating Monoamine and Organic Cation Transporters in Rats[J]. Chinese Journal of Integrative Medicine, 2021,27(3):183-191.
Zhi-hui LI, Peng-kai MA, Yun-fang HUANG, 等. Jiaotai Pill (交泰丸) Alleviates Insomnia through Regulating Monoamine and Organic Cation Transporters in Rats[J]. Chinese Journal of Integrative Medicine, 2021,27(3):183-191. DOI： 10.1007/s11655-021-3284-y.

Zhi-hui LI, Peng-kai MA, Yun-fang HUANG, et al. Jiaotai Pill (交泰丸) Alleviates Insomnia through Regulating Monoamine and Organic Cation Transporters in Rats[J]. Chinese Journal of Integrative Medicine, 2021,27(3):183-191. DOI： 10.1007/s11655-021-3284-y.

摘要

Abstract

Objective:

To reveal the effect and mechanism of Jiaotai Pill (交泰丸

JTP) on insomniac rats.

Methods:

The insomniac model was established by intraperitoneal injection of p-chlorophenylalanine (PCPA). In behavioral experiments

rats were divided into control

insomniac model

JTP [3.3 g/(kg•d)]

and diazepam [4 mg/(kg•d)]

groups. The treatment effect of JTP was evaluated by weight measurement (increasement of body weight)

open field test (number of crossings) and forced swimming test (immobility time). A high performance liquid chromatography–electrochemical detection (HPLC-ECD) method was built to determine the concentration of monoamine transmitters in hypothalamus and peripheral organs from normal

model

JTP

citalopram [30 mg/(kg•d)]

maprotiline [40 mg/(kg•d)]

and bupropion [40 mg/(kg•d)] groups. Expressions of serotonin transporter (SERT)

dopamine transporter (DAT)

and norepinephrine transporter (NET) were analyzed by quantitative polymerase chain reaction (qPCR) and Western blot in normal

model and JTP groups. A high performance liquid chromatography-electrospray ionization mass spectrometry (HPLC-ESI-MS/MS) method was established to determine the pharmacokinetics

urine cumulative excretion of metformin in vivo

and tissue slice uptake

in vitro

which were applied to assess the activity of organic cation transporters (OCTs) in hypothalamus and peripheral organs.

Results:

Compared with the insomniac model group

the body weight and spontaneous locomotor were increased

and the immobility time was decreased after treatment with JTP (

0.01). Both serotonin and dopamine contents in hypothalamus and peripheral organs were increased (

0.01). The norepinephrine content was increased in peripheral organs and decreased in hypothalamus (

0.05 or

0.01). At the same time

SERT

DAT

OCT1

OCT2

and OCT3 were down-regulated in hypothalamus and peripheral organs (

0.05). NET was down-regulated in peripheral organs and up-regulated in hypothalamus (

0.05 or

0.01). Moreover

the activity of OCTs in hypothalamus and peripheral organs was inhibited (

0.05).

Conclusion:

JTP alleviates insomnia through regulation of monoaminergic system and OCTs in hypothalamus and peripheral organs.

关键词

Keywords

Jiaotai Pillmonoaminergic systemmonoamine transmittershypothalamusperipheral organssleep disorderChinese medicine

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