Effect of Acupuncture on Ovary Morphology and Function in DHEA-Induced Polycystic Ovary Syndrome Model Rats

Dan LI; Peng BAI; Jin-yang WU; Min XIE; Rui-zhen ZHAO; Zhong-peng WANG; Xiao-han QI

doi:10.1007/s11655-021-3290-0

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Effect of Acupuncture on Ovary Morphology and Function in DHEA-Induced Polycystic Ovary Syndrome Model Rats

Acupuncture Research | Updated：2021-08-16

- Effect of Acupuncture on Ovary Morphology and Function in DHEA-Induced Polycystic Ovary Syndrome Model Rats
- Effect of Acupuncture on Ovary Morphology and Function in DHEA-Induced Polycystic Ovary Syndrome Model Rats
- Chinese Journal of Integrative Medicine 2021年27卷第3期页码：220-224
- Affiliations：
  
  1.Department of Acupuncture, the Third Affiliated Hospital, Beijing University of Chinese Medicine, Beijing (100029), China
  2.Graduate School, Beijing University of Chinese Medicine, Beijing (100029), China
  3.Department of Massage, Beijing Hepingli Hospital, Beijing (100013), China
  4.Department of Encephalopathy, the Third Affiliated Hospital, Beijing University of Chinese Medicine, Beijing (100029), China
- Author bio：
  
  Correspondence to: Prof. BAI Peng, E-mail: baipeng1978@163.com
- Funds：
  
  Scientific Research Fund of Beijing University of Chinese Medicine
- DOI：10.1007/s11655-021-3290-0
  中图分类号：
- 纸质出版日期：2021-03-01，
  
  录用日期：2020-11-24
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Dan LI, Peng BAI, Jin-yang WU, 等. Effect of Acupuncture on Ovary Morphology and Function in DHEA-Induced Polycystic Ovary Syndrome Model Rats[J]. Chinese Journal of Integrative Medicine, 2021,27(3):220-224.

Dan LI, Peng BAI, Jin-yang WU, et al. Effect of Acupuncture on Ovary Morphology and Function in DHEA-Induced Polycystic Ovary Syndrome Model Rats[J]. Chinese Journal of Integrative Medicine, 2021,27(3):220-224.
Dan LI, Peng BAI, Jin-yang WU, 等. Effect of Acupuncture on Ovary Morphology and Function in DHEA-Induced Polycystic Ovary Syndrome Model Rats[J]. Chinese Journal of Integrative Medicine, 2021,27(3):220-224. DOI： 10.1007/s11655-021-3290-0.

Dan LI, Peng BAI, Jin-yang WU, et al. Effect of Acupuncture on Ovary Morphology and Function in DHEA-Induced Polycystic Ovary Syndrome Model Rats[J]. Chinese Journal of Integrative Medicine, 2021,27(3):220-224. DOI： 10.1007/s11655-021-3290-0.

摘要

Abstract

Objective:

To investigate the effects of acupuncture on ovary morphology and function in dehydroepiandrosterone (DHEA)-induced polycystic ovary syndrome (PCOS) model rats.

Methods:

A total of 40 adult female Wistar rats were randomly allocated to 4 groups by a random number table

including control

model

metformin and acupuncture groups

10 rats in each group. PCOS rat model was developed by injecting with DHEA (6 mg/100 g body weight) in 0.2 mL of oil subcutaneously. Electrical stimulation (2 Hz

3 mA) was applied to Guanyuan (CV 4)

Zigong (EX-CA1) and Qihai (CV 6) acupoints for 30 min daily in the acupuncture group

and metformin (200 mg/kg) was given to rats in the metformin group

both once per day for 21 consecutive days

and rats in the normal group was fed with normal saline and fed regularly. After 21 days of administration

the rat blood samples were collected for detecting the reproductive hormonal levels [luteinizing hormone (LH)

follicle stimulating hormone (FSH)

estradiol (E

)

progesterone (P)

testosterone (T)] and inflammatory factors (visfatin

IL-6) analysis. Ovary tissue was used for histopathological analysis.

Results:

Compared with the model group

rats in the acupuncture and metformin groups were significantly lower in weight gain

FSH

LH and T levels

and E

and P levels significantly increased (alll

0.05). Meanwhile

LH and FSH levels were significantly decreased

and P

T and E

levels significantly increased in the acupuncture group

compared with the metformin group (

0.05). Compared with the model group

IL-6 and visfatin levels were significantly decreased in the acupuncture and metformin groups (

0.05). There were no significant differences in IL-6 and visfatin levels between the acupuncture and metformin groups (

0.05). Ovarian diameter in the acupuncture and metformin groups were smaller than the model group (

0.05). However

there were no significant differences in ovarian diameters between the acupuncture and metformin groups (

0.05).

Conclusion:

Acupuncture might improve ovary morphology and its function in DHEA-induced PCOS model rats.

关键词

Keywords

acupunctureovary morphologyovary functioninflammatory factorpolycystic ovary syndrome

references

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