Mechanism of Shengmai Injection on Anti-Sepsis and Protective Activities of Intestinal Mucosal Barrier in Mice

Juan LU; Yue YU; Xiao-jing WANG; Rui-ping CHAI; Xin-kai LYU; Ming-hui DENG; Mei-geng HU; Yun QI; Xi CHEN

doi:10.1007/s11655-021-3292-y

Your Location：

Home >

Browse articles >

Mechanism of Shengmai Injection on Anti-Sepsis and Protective Activities of Intestinal Mucosal Barrier in Mice

Original Article | Updated：2022-09-08

- Mechanism of Shengmai Injection on Anti-Sepsis and Protective Activities of Intestinal Mucosal Barrier in Mice
- Mechanism of Shengmai Injection on Anti-Sepsis and Protective Activities of Intestinal Mucosal Barrier in Mice
- Chinese Journal of Integrative Medicine 2022年28卷第9期页码：817-822
- Affiliations：
  
  Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing(100193), China
- Author bio：
  
  Prof. CHEN Xi, E-mail: chenx_implad@163.com
- Funds：
  
  National Natural Science Foundation of China(81673667);Chinese Academy of Medical Science (Peking Union Medical College) Innovation Fund for Medical Science(CIFMS;2016-I2M-3-015;2017-I2M-B&R-09)
- DOI：10.1007/s11655-021-3292-y
  中图分类号：
- 纸质出版日期：2022-09，
  
  网络出版日期：2021-07-09，
  
  录用日期：2020-06-11
Scan for full text
Juan LU, Yue YU, Xiao-jing WANG, 等. Mechanism of Shengmai Injection on Anti-Sepsis and Protective Activities of Intestinal Mucosal Barrier in Mice[J]. Chinese Journal of Integrative Medicine, 2022,28(9):817-822.

Juan LU, Yue YU, Xiao-jing WANG, et al. Mechanism of Shengmai Injection on Anti-Sepsis and Protective Activities of Intestinal Mucosal Barrier in Mice[J]. Chinese Journal of Integrative Medicine, 2022,28(9):817-822.
Juan LU, Yue YU, Xiao-jing WANG, 等. Mechanism of Shengmai Injection on Anti-Sepsis and Protective Activities of Intestinal Mucosal Barrier in Mice[J]. Chinese Journal of Integrative Medicine, 2022,28(9):817-822. DOI： 10.1007/s11655-021-3292-y.

Juan LU, Yue YU, Xiao-jing WANG, et al. Mechanism of Shengmai Injection on Anti-Sepsis and Protective Activities of Intestinal Mucosal Barrier in Mice[J]. Chinese Journal of Integrative Medicine, 2022,28(9):817-822. DOI： 10.1007/s11655-021-3292-y.

摘要

Abstract

Objective:

To study the mechanism of Shengmai Injection (SMI) on anti-sepsis and protective activities of intestinal mucosal barrier.

Methods:

The contents of 11 active components of SMI including ginsenoside Rb1

Rb2

Rb3

Rg1

Rg2

ophioposide D

schisandrol A and schisantherin A were determined using ultra-performance liquid chromatography. Fifty mice were randomly divided into the blank

the model

the low-

medium- and high-dose SMI groups (0.375

0.75

1.5 mL/kg

respectively) by random number table

10 mice in each group. In SMI group

SMI was administrated to mice daily via tail vein injection for 3 consecutive days

while the mice in the blank and model groups were given 0.1 mL of normal saline. One hour after the last SMI administration

except the blank group

the mice in other groups were intraperitoneally injected with lipopolysaccharide (LPS) saline solution (2 mL/kg) at a dosage of 5 mL/kg for development of endotoxemia mice model. The mice in the blank group were given the same volume of normal saline. Inflammatory factors including interferon-γ (INF-γ)

tumor necrosis factor-α (TNF-α)

interleukin (IL)-2 and IL-10 were measured by flow cytometry. Myosin light-chain kinase (MLCK)

nuclear factor κB (NF-κB) levels

and change of Occludin proteins in jejunum samples were analyzed by Western blot.

Results:

The decreasing trends of INF-γ

TNF-α and IL-2 were found in serum of SMI treatment groups. In SMI-treated mice

the content of Occludin increased and MLCK protein decreased compared with the model group (

0.05 or

0.01). The content of cellular and nuclear NF-κB did not change significantly (

0.05).

Conclusion:

SMI may exert its anti-sepsis activity mainly through NF-κB-pro-inflammatory factor-MLCK-TJ cascade.

关键词

Keywords

Shengmai Injectionsepsisintestinal mucosal barriermechanismChinese medicine

references

Chen X, Feng Y, Shen X, Pan G, Fan G, Gao X, et al. Anti-sepsis protection of Xuebijing Injection is mediated by differential regulation of pro- and anti-inflammatory Th17 and T regulatory cells in a murine model of polymicrobial sepsis. J Ethnopharmacol 2018;211:358-365.

Dardalas I, Stamoula E, Rigopoulos P, Malliou F, Tsaousi G, Aidoni Z, et al. Dexmedetomidine effects in different experimental sepsis in vivo models. Eur J Pharmacol 2019;856:172401.

Clark JA, Coopersmith CM. Intestinal crosstalk a new paradigm for understanding the gut as the motor of critical illness. Shock 2017;28:384-393.

Harhaj NS, Antonetti DA. Regulation of tight junctions and loss of barrier function in pathophysiology. Int J Biochem Cell Biol 2004;36:1206-1237.

Lechuga S, Ivanov AI. Disruption of the epithelial barrier during intestinal inflammation: quest for new molecules and mechanisms.Biochim Biophys Acta Mol Cell Res 2017;1864:1183-1194.

Al-Sadi R, Boivin M, Ma TY. Mechanism of cytokine modulation of epithelial tight junction barrier. Frontiers Biosci (Landmark edition) 2009;14:2765-2778.

Blikslager AT, Moeser AJ, Gookin JL, Jones SL, Odle J. Restoration of barrier function in injured intestinal mucosa. Physiol Rev 2007;2:545-564.

Niessen CM. Tight junctions/adherens junctions: basic structure and function. J Invest Dermatol 2007;127:2525-2532.

Li S, Qian Y, Xie R, Li Y, Jia Z, Zhang Z, et al. Exploring the protective effect of ShengMai-Yin and Ganmaidazao Decoction combination against type 2 diabetes mellitus with nonalcoholic fatty liver disease by network pharmacology and validation in KKAy mice. J Ethnopharmacol 2019;242:112029.

Zhang YC, Chen RM, Lu BJ, Rong YZ. Effect of Shengmai Injection on cardiac function and inflammatory reaction in patients with acute coronary syndrome. Chin J Integr Med 2008;14:107-110.

Duan B, Xie J, Rui Q, Zhang W, Xi Z. Effects of Shengmai Injection add-on therapy to chemotherapy in patients with non-small cell lung cancer: a meta-analysis. Support Care Cancer 2018;26:2103-2111.

Zhan S, Ding B, Ruan YE, Huang X, Liu G, Lv X, et al. A simple blood microdialysis in freely-moving rats for pharmacokinetic-pharmacodynamic modeling study of Shengmai injection with simultaneous determination of drug concentrations and efficacy levels in dialysate. J Pharm Biomed Anal 2018;154:23-30.

Lehmann C, Zhou J, Schuster L, Gotz F, Wegner A, Cerny V, et al.Effect of deletion of cIAP2 on intestinal microcirculation in mouse endotoxemia and polybacterial sepsis. Shock 2014;41:454-457.

Li Y, Wang F, Luo Y. Ginsenoside Rg1 protects against sepsis-associated encephalopathy through beclin 1-independent autophagy in mice. J Surg Res 2017;207:181-189.

Zou Y, Tao T, Tian Y, Zhu J, Cao L, Deng X, et al. Ginsenoside Rg1 improves survival in a murine model of polymicrobial sepsis by suppressing the inflammatory response and apoptosis of lymphocytes. J Surg Res 2013;183:760-766.

Kim JH, Yi YS, Kim MY, Cho JY. Role of ginsenosides, the main active components of Panax ginseng, in inflammatory responses and diseases. J Ginseng Res 2017;41:435-443.

Mou Z, Feng Z, Xu Z, Zhuang F, Zheng X, Li X, et al. Schisandrin B alleviates diabetic nephropathy through suppressing excessive inflammation and oxidative stress. Biochem Biophys Res Commun 2019;508:243-249.

Wang Y, Huang X, Ma Z, Wang Y, Chen X, Gao Y. Ophiopogonin D alleviates cardiac hypertrophy in rat by upregulating CYP2J3 in vitro and suppressing inflammation in vivo. Biochem Biophys Res Commun 2018;503:1011-1019.

Zhang Q, Lenardo MJ, Baltimore D. 30 years of NF-kappa B: a blossoming of relevance to human pathobiology. Cell 2017;168:37-57.

Goebeler M, Gillitzer R, Kilian K, Utzel K, Bröcker EB, Rapp UR, et al. Multiple signaling pathways regulate NF-kappa B-dependent transcription of the monocyte chemoattractant protein-1 gene in primary endothelial cells. Blood 2001;91:46-55.

Cong X, Kong W. Endothelial tight junctions and their regulatory signaling pathways in vascular homeostasis and disease. Cellular Signalling 2020;66:109485.

Cole WC, Welsh DG. Role of myosin light chain kinase and myosin light chain phosphatase in the resistance arterial myogenic response to intravascular pressure. Arch Biochem Biophysics 2011;510:160-173.

Boivin MA, Roy PK, Bradley A, Kennedy JC, Rihani T, Ma TY.Mechanism of interferon-gamma-induced increase in T84 intestinal epithelial tight junction. J Interferon Cytokine Res 2009;29:45-54.

Sappington PL, Yang R, Yang H, Tracey KJ, Delude RL, Fink MP.HMGB1 B box increases the permeability of Caco-2 enterocytic monolayers and impairs intestinal barrier function in mice.Gastroenterology 2002;123:790-802.

Han X, Fink MP, Yang R, Delude RL. Increased iNOS activity is essential for intestinal epithelial tight junction dysfunction in endotoxemic mice. Shock 2004;21:261-270.

More>

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Mechanism Study on Chinese Medicine in Treatment of Nodular Goiter