针刺对急性心肌缺血模型大鼠心包经和心脏微循环灌注量的影响

Yi ZHUANG; Jie ZHOU; Yu-mei ZHOU; Jiao CHEN; Ping WU; Pei-ran LYU; Min WAN; Liao-jun LUO; Ding-jun CAI; Fan-rong LIANG

doi:10.1007/s11655-021-3294-9

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针刺对急性心肌缺血模型大鼠心包经和心脏微循环灌注量的影响

Acupuncture Research | Updated：2022-03-18

- 针刺对急性心肌缺血模型大鼠心包经和心脏微循环灌注量的影响
- Influence of Acupuncture on Microcirculation Perfusion of Pericardium Meridian and Heart in Acute Myocardial Ischemia Model Rats
- Chinese Journal of Integrative Medicine 2022年28卷第1期页码：69-75
- Affiliations：
  
  1.College of Acupuncture, Moxibustion and Tuina, Chengdu University of Traditional Chinese Medicine, Chengdu (610075),China
  2.College of Acupuncture, Moxibustion and Tuina,Nanjing University of Chinese Medicine, Nanjing (210023), China
  3.Department of Acupuncture and Moxibustion, Xin Du Hospital of Traditional Chinese Medicine, Chengdu (610599), China
  4.The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen ,Guangdong Province (518005), China
- Author bio：
- Funds：
  
  the Project Acupoint Sensitization Research of the National Natural Science Foundation of China(81590951);the State Key Program for Basic Research of China(2012CB518501);the National Natural Science Foundation of China(81373559)
- DOI：10.1007/s11655-021-3294-9
  中图分类号：
- 纸质出版日期：2022-01-01，
  
  网络出版日期：2021-11-24，
  
  录用日期：2020-08-12
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Yi ZHUANG, Jie ZHOU, Yu-mei ZHOU, 等. 针刺对急性心肌缺血模型大鼠心包经和心脏微循环灌注量的影响[J]. Chinese Journal of Integrative Medicine, 2022,28(1):69-75.

Yi ZHUANG, Jie ZHOU, Yu-mei ZHOU, et al. Influence of Acupuncture on Microcirculation Perfusion of Pericardium Meridian and Heart in Acute Myocardial Ischemia Model Rats[J]. Chinese Journal of Integrative Medicine, 2022,28(1):69-75.
Yi ZHUANG, Jie ZHOU, Yu-mei ZHOU, 等. 针刺对急性心肌缺血模型大鼠心包经和心脏微循环灌注量的影响[J]. Chinese Journal of Integrative Medicine, 2022,28(1):69-75. DOI： 10.1007/s11655-021-3294-9.

Yi ZHUANG, Jie ZHOU, Yu-mei ZHOU, et al. Influence of Acupuncture on Microcirculation Perfusion of Pericardium Meridian and Heart in Acute Myocardial Ischemia Model Rats[J]. Chinese Journal of Integrative Medicine, 2022,28(1):69-75. DOI： 10.1007/s11655-021-3294-9.

摘要

目的:

观察针刺对急性心肌缺血大鼠心包经和心脏微循环灌注量的影响

评价针刺是否能同时影响经络和相应脏器. 并通过比较针刺不同经络的穴位

讨论其是否具有相同作用.

方法:

采用结扎大鼠左前降支的方法建立急性心肌缺血模型. 将32只大鼠随机分为4组

急性心肌梗死组、针刺内关组、针刺列缺组及针刺前三里组

每组8只. 另8只大鼠仅接受开胸手术作为假手术组. 针刺组大鼠分别在内关、列缺和前三里穴进行手针干预15分钟. 采用激光散斑灌注成像仪 (LPSI) 监测针刺前后心包经和心脏的微循环灌注量

并用PSI系统计算和分析灌注单位 (PU) .

结果:

与针刺前比较

造模后急性心肌缺血组大鼠心脏微循环灌注量 (HMP) 在针刺期间 (

0.05) 和针刺后 (

0.05) 持续下降

心包经微循环灌注量 (PMP) 在3个阶段未见明显差异 (

0.05) . 与针刺前比较

内关组在针刺过程中PMP和HMP均明显升高

针刺后PMP明显降低 (均

0.05) ; 列缺组及前三里组PMP稍有升高

差异无统计学意义 (

0.05)

针刺干预后

上述指标明显降低 (

0.05) . 此外

前三里组HMP在针刺期间明显降低

尤其与针刺前比较

差异明显 (

0.05) .

结论:

针刺内关穴可明显提高急性心肌缺血模型大鼠心包经和心脏微循环灌注量

其作用优于列缺穴和前三里穴. 进一步证实针刺促进气血流注

并且具有穴位特异性与经络传播效应.

Abstract

Objective:

To observe the influence of acupuncture on microcirculation perfusion of the pericardium meridian and heart in acute myocardial ischemia (AMI) rats and evaluate whether acupuncture can simultaneously affect the meridians and corresponding viscera. Additionally

acupoints at different meridians were compared and whether they exert the same effects was discussed.

Methods:

Totally 32 Sprague-Dawley rats were subjected to left anterior descending (LAD) ligation to develop an AMI model. Rats were divided into 4 groups

including AMI

acupuncture Neiguan (PC 6)

Lieque (LU 7) and Qiansanli (LI 10) groups (

=8). Eight rats received only thoracotomy (sham-operated group). The rats in the acupuncture groups received manual acupuncture at PC 6

LU 7 and LI 10 acupoints for 15 min

respectively. The microcirculation perfusion of pericardium meridian and heart was monitored by laser speckle perfusion imager (LSPI) before

during and after acupuncture manipulation for 15 min. Subsequently

the perfusion unit (PU) was calculated and analyzed by PSI System.

Results:

After LAD

compared to pre-acupuncture stage

the heart microcirculation perfusion (HMP) in the AMI group decreased continuously at during-acupuncture (

0.05) and post-acupuncture stages (

0.05)

and the pericardium meridian microcirculation perfusion (PMP) showed no significant differences at 3 stages (

0.05). Compared to pre-acupuncture stage

the PMP and HMP in PC 6 group significantly increased during acupuncture manipulation (both

0.05)

and PMP decreased obviously after acupuncture (

0.05). The PMP in the LU 7 and LI 10 groups were slightly elevated (both

0.05); however

they were significantly reduced after acupuncture manipulation (both

0.05). Additionally

HMP of LI 10 group was decreased significantly during acupuncture

especially compared to pre-acupuncture stage (

0.05).

Conclusions:

Acupuncture at PC 6 obviously increased the PMP and HMP in AMI rats

and the effects were superior to at LU 7 and LI 10 acupoints. It was further confirmed that acupuncture promoted qi and blood circulation

indicating that acupoint specificity exists and features a meridian-propagated effect.

关键词

Keywords

microcirculation perfusionacupuncturepericardium meridianheartacute myocardial ischemia

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