黄芪多糖联合小檗碱治疗高脂饮食诱导的小鼠肥胖和肠道菌群调控作用研究

YUE Shi-jun; WANG Wen-xiao; ZHANG Lei; LIU Juan; FENG Wu-wen; GAO Huan; TANG Yu-ping; YAN Dan

doi:10.1007/s11655-021-3303-z

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黄芪多糖联合小檗碱治疗高脂饮食诱导的小鼠肥胖和肠道菌群调控作用研究

Original Article | Updated：2023-06-27

- 黄芪多糖联合小檗碱治疗高脂饮食诱导的小鼠肥胖和肠道菌群调控作用研究
- Anti-obesity and Gut Microbiota Modulation Effect of Astragalus Polysaccharides Combined with Berberine on High-Fat Diet-Fed Obese Mice^*
- 中国结合医学杂志（英文版） 2023年29卷第7期页码：617-625
- Affiliations：
  
  1.Beijing Key Laboratory of Bio-characteristic Profiling for Evaluation of Rational Drug Use, Beijing Shijitan Hospital,Capital Medical University, Beijing (100038), China
  2.Key Laboratory of Shaanxi Administration of Traditional Chinese Medicine for TCM Compatibility, Shaanxi University of Chinese Medicine, Xi'an (712046), China
  3.Department of Pharmacy,Beijing Friendship Hospital, Captical University, Beijing Institute of Clinical Pharmacy, Beijing (100050), China
- Author bio：
  
  Prof. YAN Dan, E-mail: yd277@126.com
- Funds：
  
  the National Natural Science Foundation of China(82130112;81903786;81773891);Shaanxi Administration of Traditional Chinese Medicine(2019-ZZ-JC018);the National Great New Drugs Development Project of China(2017ZX09301-040);the Natural Science Foundation of Shaanxi Province(2019JQ-054)
- DOI：10.1007/s11655-021-3303-z
  中图分类号：
- 纸质出版日期：2023-07，
  
  网络出版日期：2021-12-18，
  
  录用日期：2021-02-18
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黄芪多糖联合小檗碱治疗高脂饮食诱导的小鼠肥胖和肠道菌群调控作用研究[J]. 中国结合医学杂志（英文版）, 2023,29(7):617-625.

YUE Shi-jun, WANG Wen-xiao, ZHANG Lei, et al. Anti-obesity and Gut Microbiota Modulation Effect of Astragalus Polysaccharides Combined with Berberine on High-Fat Diet-Fed Obese Mice^*[J]. Chinese Journal of Integrative Medicine, 2023,29(7):617-625.
黄芪多糖联合小檗碱治疗高脂饮食诱导的小鼠肥胖和肠道菌群调控作用研究[J]. 中国结合医学杂志（英文版）, 2023,29(7):617-625. DOI： 10.1007/s11655-021-3303-z.

YUE Shi-jun, WANG Wen-xiao, ZHANG Lei, et al. Anti-obesity and Gut Microbiota Modulation Effect of Astragalus Polysaccharides Combined with Berberine on High-Fat Diet-Fed Obese Mice^*[J]. Chinese Journal of Integrative Medicine, 2023,29(7):617-625. DOI： 10.1007/s11655-021-3303-z.

摘要

目的:

探讨黄芪多糖 (APS) 联合小檗碱 (BBR) 治疗高脂饮食 (HFD) 诱导的小鼠肥胖作用.

方法:

除正常小鼠外

32只HFD诱导的肥胖小鼠被随机分为HFD组、APS组 (1

000 mg/kg APS) 、BBR组 (200 mg/kg BBR) 和APS+BBR组 (1

000 mg/kg APS + 200 mg/ kg BBR) . 治疗6周后 (每天灌胃一次)

收集小鼠附睾脂肪、肝脏和结肠组织经苏木精-伊红染色后行病理学检查

并采用自动化分析仪检测血清生化指标. 第12周收集小鼠粪便

通过16S核糖体核糖核酸 (16S rRNA) 测序分析肠道菌群的分类和功能特征.

结果:

与HFD组比较

APS+BBR组小鼠平均体重明显降低 (

0.01)

伴随着脂肪堆积减少

结肠完整性、胰岛素敏感性和葡萄糖稳态增强 (

0.05或

0.01) . APS联合BBR在改善HFD诱导的胰岛素抵抗方面较单独使用APS或BBR更有效 (

0.05或

0.01) . 粪便样本的16S rRNA测序结果表明

APS联合BBR对HFD诱导的肥胖小鼠肠道菌群失调呈现出更有益的影响

主要是通过丰富拟杆菌属的相对丰度

并且与碳水化合物代谢相关的细菌基因数目的增加趋势相对应.

结论:

APS联合BBR可协同治疗HFD诱导的小鼠肥胖并调节肠道菌群.

Abstract

Objective:

To investigate whether astragalus polysaccharides (APS) combined with berberine (BBR) can reduce high-fat diet (HFD)-induced obesity in mice.

Methods:

Except for normal mice

32 HFD-induced obese mice were randomized into HFD

APS (1

000 mg/kg APS)

BBR (200 mg/kg BBR)

and APS plus BBR (1

000 mg/kg APS plus 200 mg/kg BBR) groups

respectively. After 6-week treatment (once daily by gavage)

the obesity phenotype and pharmacodynamic effects were evaluated by histopathological examination of epididymal fat

liver

and colon using hematoxylin-eosin staining and serum biochemical analyses by an automated chemistry analyzer. The feces were collected at the 12 th week

and taxonomic and functional profiles of gut microbiota were analyzed by 16S ribosomal ribonucleic acid (16S rRNA) sequencing.

Results:

Compared with HFD group

the average body weight of APS plus BBR group was decreased (

0.01)

accompanied with the reduced fat accumulation

enhanced colonic integrity

insulin sensitivity and glucose homeostasis (

0.05 or

0.01). Importantly

APS combined with BBR treatment was more effective than APS or BBR alone in improving HFD-induced insulin resistance (

0.05 or

0.01). 16S rRNA sequence-based analysis of fecal samples demonstrated that APS combined with BBR treatment exhibited a better impact on HFD-induced gut microbiota dysbiosis

exclusively via the enriched abundances of

Bacteroides

which corresponded to the large increase of predicted bacterial genes involved in carbohydrate metabolism.

Conclusion:

APS combined with BBR may synergistically reduce obesity and modulate the gut microbiota in HFD-fed mice.

关键词

Keywords

Astragalus polysaccharidesberberineobesitygut microbiotaBacteroides

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