Mechanisms of Dangua Recipe in Improving Glycolipid Metabolic Disorders Based on Transcriptomics

Xian-pei HENG; Zhi-ta WANG; Liang LI; Liu-qing YANG; Su-ping HUANG

doi:10.1007/s11655-021-3337-2

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Mechanisms of Dangua Recipe in Improving Glycolipid Metabolic Disorders Based on Transcriptomics

Original Article | Updated：2022-02-22

- Mechanisms of Dangua Recipe in Improving Glycolipid Metabolic Disorders Based on Transcriptomics
- Mechanisms of Dangua Recipe in Improving Glycolipid Metabolic Disorders Based on Transcriptomics
- Chinese Journal of Integrative Medicine 2022年28卷第2期页码：130-137
- Affiliations：
  
  1.Department of Endocrinology, People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine, Fuzhou (350004),China
  2.Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou (350122), China
- Author bio：
  
  Prof. HENG Xian-pei, E-mail: hengxianpei@hotmail.com
- Funds：
  
  the National Natural Science Foundation of China(81873213;81473550);the Natural Science Foundation of Fujian Province(2017J01213;2016J0146);the Innovation Fund of Medical Science of Fujian Province, China(2017-CX-42)
- DOI：10.1007/s11655-021-3337-2
  中图分类号：
- 纸质出版日期：2022-02-01，
  
  网络出版日期：2021-11-10，
  
  录用日期：2020-02-13
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Xian-pei HENG, Zhi-ta WANG, Liang LI, 等. Mechanisms of Dangua Recipe in Improving Glycolipid Metabolic Disorders Based on Transcriptomics[J]. Chinese Journal of Integrative Medicine, 2022,28(2):130-137.

Xian-pei HENG, Zhi-ta WANG, Liang LI, et al. Mechanisms of Dangua Recipe in Improving Glycolipid Metabolic Disorders Based on Transcriptomics[J]. Chinese Journal of Integrative Medicine, 2022,28(2):130-137.
Xian-pei HENG, Zhi-ta WANG, Liang LI, 等. Mechanisms of Dangua Recipe in Improving Glycolipid Metabolic Disorders Based on Transcriptomics[J]. Chinese Journal of Integrative Medicine, 2022,28(2):130-137. DOI： 10.1007/s11655-021-3337-2.

Xian-pei HENG, Zhi-ta WANG, Liang LI, et al. Mechanisms of Dangua Recipe in Improving Glycolipid Metabolic Disorders Based on Transcriptomics[J]. Chinese Journal of Integrative Medicine, 2022,28(2):130-137. DOI： 10.1007/s11655-021-3337-2.

摘要

目的:

基于转录组学研究丹瓜方改善糖脂的作用机制.

方法:

选取血糖正常的SD大鼠

按照随机数字表随机分为3组: A组为常规组

C组用高热量饲料造模

B组用高热量饲料造模同时给予丹瓜方20.5g/ (Kg鼠) 进行干预. 12周后取肝组织检测. 通过基因序列和转录组学分析找出反映丹瓜方疗效的糖脂代谢关键基因

并通过肝组织基因或蛋白质进行鉴定. 用酶联免疫法检测肝组织烟酰胺磷酸核糖转移酶 (Nampt) 和磷酸烯醇式丙酮酸羧激酶 (PEPCK) 蛋白

WB法检测脂肪酸合成酶 (FASN)

qRT-PCR法检测脂肪酸结合蛋白5 (FABP5) mRNA表达. 随后用Nampt阻断剂GEN-617注射糖尿病模型鼠以验证丹瓜方的作用靶点

检测血浆糖化血红蛋白 (HbA1c) 、甘油三酯(TG)、胆固醇(TC)水平.

结果:

A组与C组间检测出257个差显基因

B组与C组间有392个差显基因. 肝组织验证Nampt、FASN、PEPCK蛋白

FABP1-mRNA表达与转录组学一致. 体内功能验证显示

应用GEN-617增加了糖尿病模型鼠体重和HbA1c、TC、TG水平(

0.05或

0.01); 联合应用丹GEN-617和丹瓜方后

上述指标除TG外均下降(

0.05或

0.01).

结论:

激活烟酰胺磷酸核糖转移酶是丹瓜方改善糖脂代谢的机制之一.

Abstract

Objective:

To explore the mechanisms of Dangua Recipe (DGR) in improving glycolipid metabolism based on transcriptomics.

Methods:

Sprague-Dawley rats with normal glucose level were divided into 3 groups according to a random number table

including a conventional diet group (Group A)

a DGR group (Group B

high-calorie diet + 20.5 g DGR)

and a high-calorie fodder model group (Group C). After 12 weeks of intervention

the liver tissue of rats was taken. Gene sequence and transcriptional analysis were performed to identify the key genes related to glycolipid metabolism reflecting DGR efficacy

and then gene or protein validation of liver tissue were performed. Nicotinamide phosphoribosyl transferase (Nampt) and phosphoenolpyruvate carboxykinase (PEPCK) proteins in liver tissues were detected by enzyme linked immunosorbent assay

fatty acid synthase (FASN) protein was detected by Western blot

and fatty acid binding protein 5 (FABP5)-mRNA was detected by quantitative real-time polymerase chain reaction. Furthermore

the functional verification was performed on the diabetic model rats by Nampt blocker (GEN-617) injected in vivo. Hemoglobin A

(HbA

)

plasma total cholesterol and triglycerides were detected.

Results:

Totally

257 differentialdominant genes of Group A vs. Group C and 392 differential-dominant genes of Group B vs. Group C were found. Moreover

11 Gene Ontology molecular function terms and 7 Kyoto Encyclopedia of Genes and Genomes enrichment pathways owned by both Group A

. Group C and Group C

. Group B were confirmed. The liver tissue target validation showed that Nampt

FASN

PEPCK protein and FABP5-mRNA had the same changes consistent with transcriptome. The

in vivo

functional tests showed that GEN-617 increased body weight

HbA

triglyceride and total cholesterol levels in the diabetic rats (

0.05 or

0.01); while all the above-mentioned levels (except triglyceride) were decreased significantly by GEN-617 combined with DGR intervention (

0.05 or

0.01).

Conclusion:

Nampt activation was one of the mechanisms about DGR regulating glycolipid metabolism.

关键词

Keywords

transcriptomicsDangua Recipeglycolipid metabolismNampttherapeutic targetChinese medicine

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