Effect of Electro-acupuncture on Vasomotor Symptoms in Rats with Acute Cerebral Infarction Based on Phosphatidylinositol System

Jing LI; Ying HE; Yuan-hao DU; Min ZHANG; Rainer Georgi; Bernhard Kolberg; Dong-wei SUN; Kun MA; Yong-feng LI; Xue-zhu ZHANG

doi:10.1007/s11655-021-3341-6

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Effect of Electro-acupuncture on Vasomotor Symptoms in Rats with Acute Cerebral Infarction Based on Phosphatidylinositol System

Acupuncture Research | Updated：2022-02-22

- Effect of Electro-acupuncture on Vasomotor Symptoms in Rats with Acute Cerebral Infarction Based on Phosphatidylinositol System
- Effect of Electro-acupuncture on Vasomotor Symptoms in Rats with Acute Cerebral Infarction Based on Phosphatidylinositol System
- Chinese Journal of Integrative Medicine 2022年28卷第2期页码：145-152
- Affiliations：
  
  1.Key Laboratory of Acupuncture of Tianjin, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine,National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin (300381), China
  2.Department of Acupuncture and Moxibustion, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin (300381), China
  3.Department of Chemical Biology,Max Planck Institute for Medical Research, Heidelberg (69120),Germany
  4.Department of Internal Medicine, Mannheim Medical School of Heidelberg University, Mannheim (68167),Germany
  5.Department of Chinese Medicine Rehabilitation,Shenzhen Baoan District Hospital of Traditional Chinese Medicine, Shenzhen, Guangdong Province (518000), China
  6.Department of Preventive Treatment of Disease, Binhai New Area Hospital of Traditional Chinese Medicine, Fourth Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin (300193), China
  7.Institute of Acupuncture and Moxibustion, Shaanxi University of Chinese Medicine, Xi'an(712046), China
- Author bio：
  
  Prof. DU Yuan-hao, E-mail: ddyh64@tjutcm.edu.cn
- Funds：
  
  the National Natural Science Foundation of China(82074543;81473765;81674056);the Tianjin Municipal Natural Science Foundation(18JCYBJC94200)
- DOI：10.1007/s11655-021-3341-6
  中图分类号：
- 纸质出版日期：2022-02-01，
  
  网络出版日期：2021-12-07，
  
  录用日期：2021-01-08
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Jing LI, Ying HE, Yuan-hao DU, 等. Effect of Electro-acupuncture on Vasomotor Symptoms in Rats with Acute Cerebral Infarction Based on Phosphatidylinositol System[J]. Chinese Journal of Integrative Medicine, 2022,28(2):145-152.

Jing LI, Ying HE, Yuan-hao DU, et al. Effect of Electro-acupuncture on Vasomotor Symptoms in Rats with Acute Cerebral Infarction Based on Phosphatidylinositol System[J]. Chinese Journal of Integrative Medicine, 2022,28(2):145-152.
Jing LI, Ying HE, Yuan-hao DU, 等. Effect of Electro-acupuncture on Vasomotor Symptoms in Rats with Acute Cerebral Infarction Based on Phosphatidylinositol System[J]. Chinese Journal of Integrative Medicine, 2022,28(2):145-152. DOI： 10.1007/s11655-021-3341-6.

Jing LI, Ying HE, Yuan-hao DU, et al. Effect of Electro-acupuncture on Vasomotor Symptoms in Rats with Acute Cerebral Infarction Based on Phosphatidylinositol System[J]. Chinese Journal of Integrative Medicine, 2022,28(2):145-152. DOI： 10.1007/s11655-021-3341-6.

摘要

目的:

通过观察肌醇磷脂系统相关因子表达的变化

探讨电针对急性脑梗死大鼠血管舒缩症状的影响.

方法:

42只Wistar大鼠按随机数字表法分为3组: 对照组 (

=6) 、模型组 (

=18) 和电针组 (

=18) . 电针组采用大脑中动脉闭塞法造模后即刻给予水沟穴电针治疗

模型组和对照组不进行任何治疗. 使用神经严重程度评分评估神经功能缺损的程度

通过激光扫描共聚焦显微镜评估脑血流量

通过蛋白质印迹分析法检测Gq和CaM的表达水平. 通过蛋白质结合竞争法检测IP3的表达水平

通过薄层定量分析法检测DAG的表达水平

通过流式细胞仪检测细胞内游离钙离子浓度的表达水平.

结果:

3、6h模型组在MCAO后NSS评分显著高于对照组 (

0.01)

6h电针组显着低于模型组 (

0.01) . 1、3、6 h模型组在MCAO后脑血流量显著低于对照组 (

0.01)

而电针组显著高于同时相模型组 (

0.01). 模型组Gq、CaM、IP3、DAG、[Ca

]

的表达下显著高于对照组 (

0.05或

0.01)

而电针组显著低于同时相模型组 (

0.05或

0.01) .

结论:

电针水沟穴可有效抑制急性脑梗死大鼠肌醇磷脂系统相关因子的过度表达

改善脑血管的自律运动、缓解脑血管的痉挛.

Abstract

Objective:

To investigate the effect of electro-acupuncture (EA) on vasomotor symptoms in rats with acute cerebral infarction

by observing the changes in the expression of factors related to the phosphatidylinositol (PI) system.

Methods:

Forty-two Wistar rats were randomly divided into 3 groups by a random number table: the control group (

=6)

the model group (

=18) and the EA group (

=18). The EA group was given EA treatment at Shuigou (GV 26) instantly after modeling with middle cerebral artery occlusion (MCAO) method

while the model and control groups were not given any treatment. The degrees of neurological deficiency were evaluated using neurological severity scores (NSS) and the brain blood flow was evaluated by a laser scanning confocal microscope. Western blot analysis was conducted to detect the expression levels of G-protein subtype (Gq) and calmodulin (CaM). Competition for protein binding was conducted to detect the expression level of inositol triphosphate (IP3). Thin layer quantitative analysis was conducted to detect the expression level of diacylglycerol (DAG). The expression level of intracellular concentration of free calcium ion ([Ca

]

) was detected by flow cytometry.

Results:

The NSS of the model group was significantly higher than the control group at 3 and 6 h after MCAO (

0.01)

while the EA group was significantly lower than the model group at 6 h (

0.01). The cerebral blood flow in the model group was significantly lower than the control group at 1

3 and 6 h after MCAO (

0.01)

while for the EA group it was remarkably higher than the model group at the same time points (

0.01). The expressions of Gq

CaM

IP3

DAG and [Ca

]

in the model group were significantly higher than the control group (

0.05 or

0.01)

and those in the EA group were significantly lower than the model group at the same time points (

0.05 or

0.01).

Conclusion:

EA treatment at GV 26 can effectively decrease the over-expression of related factors of PI system in rats with acute cerebral infarction

improve cerebral autonomy movement

and alleviate cerebral vascular spasm.

关键词

Keywords

cerebral infarctionphosphatidylinositol systemvasomotionShuigou (GV 26)electroacupunctureChinese medicine

references

Shin TH, Lee DY, Basith S, Manavalan B, Paik MJ, Rybinnik I,et al. Metabolome changes in cerebral ischemia. Cells 2020;9:1630.

Li L, Jiang Q, Zhang L, Ding GL, Zhang ZG, Li QJ, et al.Angiogenesis and improved cerebral blood flow in the ischemic boundary area detected by MRI after administration of sildenafil to rats with embolic stroke. Brain Res 2017;1132:185-192.

Li J, He JJ, Du YH, Cui JJ, Ma Y, Zhang XZ. Electroacupuncture improves cerebral blood flow and attenuates moderate ischemic injury via angiotensin Ⅱ its receptors-mediated mechanism in rats. BMC Complement Altern Med 2014;14:441.

Han QD, Wen YY, eds. Vascular biology. Beijing: Beijing Medical University & China Union Medical University Press;1997:74-79.

Du YH, Shi L, Li J, Xiong J, Li B, Fan XN. Angiogenesis and improved cerebral blood flow in the ischemic boundary area were detected after electroacupuncture treatment to rats with ischemic stroke. Neurol Res 2011;1:101-107.

Lu Y, Du YH, Xu YL, Gao L, Cui JJ, Yang LH, et al. Study on expression of PKC in vascular smooth muscle of rats with middle cerebral artery occlusion and interfering effect of electro-acupuncture at Shuigou. China J Tradit Chin Med Pharm (Chin)2013;28:2310-2314.

Lu Y, Du YH, Xu YL, Gao L, Cui JJ, Yang LH, et al. Effect of electro-acupuncture at "Shuigou" (GV 26) on immunoactivity and content of protein kinase C in the middle cerebral artery in acute cerebral infarction rats. Acupunct Res (Chin) 2015;40:219-223.

Longa EZ, Weinstein PR, Carlson S, Cummins R. Reversible middle cerebral artery occlusion without craniectomy in rats.Stroke 1989;1:84-91.

Hua XB, Zhou HL. Development of acupoint atlas of rats. Lab Animals Animal Exp (Chin) 1991;1:1-5.

Chen J, Li Y, Wang L, Zhang Z, Lu D, Lu M, et al. Therapeutic benefit of intravenous administration of bone marrow stromal cells after cerebral ischemia in rats. Stroke 2001;32:1005-1011.

Najm FJ, Madhavan M, Zaremba A, Shick E, Karl RT, Factor DC,et al. Drug-based modulation of endogenous stem cells promotes functional remyelination in vivo. Nature 2015;522:216-220.

Wang QL, Xie J, Liang J, Dong GT, Qing LS. Competitive protein binding assay of naproxen by human serum albumin functionalized silicon dioxide nanoparticles. Molecules 2019;24:2593.

Zhao Y, Wang C, Qiu B, Li C, Wang H, Jin H, et al. Passive immunotherapy for Middle East respiratory syndrome coronavirus infection with equine immunoglobulin or immunoglobulin fragments in a mouse model. Antiviral Res 2017;137:125-130.

Deng YJ, Yi SX, Lin YP, Yan J, Guo H, Xiang ZY, et al. Effects of acupuncture at acupoints of the Foot Yangming meridian on calcium ion concentration of smooth muscle cells in gastric autrum in the rabbit. J Tradit Chin Med (Chin) 2004;15:492-494.

Lin X, Du YH. Effect of electroacupuncture on cAMP of cerebral vascular artery smooth muscle cells in rats with acute cerebral infarction. Chin J Basic Med Tradit Chin Med (Chin)2007;13:860-862.

Guan L, Du YH. Effect of acupuncture on automatic rhythmicity in microvasculature of cerebral pia mater in rats with local infarction.Tianjin J Tradit Chin Med (Chin) 2006;3:209-210.

Xiong W, Zhao CM, An LX, Xie SN, Jia CR. Efficacy of acupuncture combined with local anesthesia in ischemic stroke patients with carotid artery stenting: a prospective randomized trial. Chin J Integr Med 2020;26:609-616.

Jover-Mengual T, Castelló-Ruiz M, Burguete MC, Jorques M,López-Morales MA, Aliena-Valero A, et al. Molecular mechanisms mediating the neuroprotective role of the selective estrogen receptor modulator, bazedoxifene, in acute ischemic stroke: a comparative study with 17β-estradiol. J Steroid Biochem Mol Biol 2017;171:296-304.

Oldham WM, Hamm HE. Heterotrimeric G protein activation by G-protein-coupled receptors. Nat Rev Mol Cell Biol 2008;9:60-71.

Qian J, Gu YY, Wu C, Yu F, Chen YQ, Zhu JM, et al. Agonist-induced activation of human FFA1 receptor signals to extracellular signal-regulated kinase 1 and 2 through Gq- and Gi-coupled signaling cascades. Cell Mol Biol Lett 2017;22:13.

Patt J, Alenfelder J, Pfeil EM, Voss JH, Merten N, Eryilmaz F, et al.An experimental strategy to probe Gq contribution to signal transduction in living cells. J Biol Chem 2021;296:100472.

Kankanamge D, Ubeysinghe S, Tennakoon M, Pantula PD, Mitra K, Giri L, et al. Dissociation of the G protein βγ from the Gq-PLCβ complex partially attenuates PIP2 hydrolysis. J Biol Chem 2021;296:100702.

Nassar NN, Abdel-Rahman AA. Brain stem adenosine receptors modulate centrally mediated hypotensive responses in conscious rats: a review. J Adv Res 2015;3:331-340.

Litosch I. Regulating G protein activity by lipase-independent functions of phospholipase C. Life Sci 2015;137:116-124.

Sun DW, Du YH, Shi L, Liu WH. Effect of electroacupuncture on inositol triphosphate and diacylglycerol levels in cerebral arteries of cerebral ischemia rats. Acupunct Res (Chin)2008;33:392-396.

Singer HA. Ca2+/calmodulin-dependent protein kinase Ⅱ function in vascular remodelling. J Physiol 2012;590:1349-1356.

Saddouk FZ, Ginnan R, Singer HA. Ca2+/calmodulin-dependent protein kinase Ⅱ in vascular smooth muscle. Adv Pharmacol 2017;78:171-202.

Saddouk FZ, Sun LY, Liu YF, Jiang M, Singer DV, Backs J, et al.Ca2+/calmodulin-dependent protein kinase Ⅱ-γ (CaMKⅡγ)negatively regulates vascular smooth muscle cell proliferation and vascular remodeling. FASEB J 2016;30:1051-1064.

Tebar F, Chavero A, Agell N, Lu A, Rentero C, Enrich C, et al.Pleiotropic roles of calmodulin in the regulation of KRas and Rac1 GTPases: functional diversity in health and disease. Int J Mol Sci 2020;21:3680.

Aseervatham J, Li X, Mitchell CK, Lin YP, Heidelberger R,O'Brien J. Calmodulin binding to connexin 35: specializations to function as an electrical synapse. Int J Mol Sci 2020;21:6346.

Tzortzopoulos A, Best SL, Kalamida D, Török K. Ca2+/calmodulin-dependent activation and inactivation mechanisms of alphaCaMKⅡ and phospho-Thr286-alphaCaMKⅡ. Biochemistry 2004;43:6270-6280.

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