Inokosterone Is a Potential Drug Target of Estrogen Receptor 1 in Rheumatoid Arthritis Patients:Analysis from Active Ingredient of <italic style="font-style: italic">Cyathula Officinalis</italic>

Ji-hao MO; Han-kun XIE; Ye-mian ZHOU; Ng Sihan-benjamin; Shao-xia LI; Lei WANG

doi:10.1007/s11655-021-3492-5

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Inokosterone Is a Potential Drug Target of Estrogen Receptor 1 in Rheumatoid Arthritis Patients:Analysis from Active Ingredient of Cyathula Officinalis

Original Article | Updated：2021-10-18

- Inokosterone Is a Potential Drug Target of Estrogen Receptor 1 in Rheumatoid Arthritis Patients:Analysis from Active Ingredient of Cyathula Officinalis
- Inokosterone Is a Potential Drug Target of Estrogen Receptor 1 in Rheumatoid Arthritis Patients:Analysis from Active Ingredient of Cyathula Officinalis
- Chinese Journal of Integrative Medicine 2021年27卷第10期页码：767-773
- Affiliations：
  
  1.Department of Medical Laboratory, Luoyang Orthopedic Hospital of Henan Province, Orthopedic Institute of Henan Province, Luoyang, Henan Province (471002), China
  2.Miller School of Medicine, University of Miami (33136), Miami, FL,USA
  3.Institute of Technical Biology & Agriculture Engineering,Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei (230031), China
  4.Eatobe Pte. Limited(573969), Singapore
- Author bio：
  
  Prof. WANG Lei, E-mail: lxw561@miami.edu
- Funds：
- DOI：10.1007/s11655-021-3492-5
  中图分类号：
- 纸质出版日期：2021-10-01，
  
  网络出版日期：2021-08-25，
  
  录用日期：2020-05-11
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Ji-hao MO, Han-kun XIE, Ye-mian ZHOU, 等. Inokosterone Is a Potential Drug Target of Estrogen Receptor 1 in Rheumatoid Arthritis Patients:Analysis from Active Ingredient of Cyathula Officinalis[J]. Chinese Journal of Integrative Medicine, 2021,27(10):767-773.

Ji-hao MO, Han-kun XIE, Ye-mian ZHOU, et al. Inokosterone Is a Potential Drug Target of Estrogen Receptor 1 in Rheumatoid Arthritis Patients:Analysis from Active Ingredient of Cyathula Officinalis[J]. Chinese Journal of Integrative Medicine, 2021,27(10):767-773.
Ji-hao MO, Han-kun XIE, Ye-mian ZHOU, 等. Inokosterone Is a Potential Drug Target of Estrogen Receptor 1 in Rheumatoid Arthritis Patients:Analysis from Active Ingredient of Cyathula Officinalis[J]. Chinese Journal of Integrative Medicine, 2021,27(10):767-773. DOI： 10.1007/s11655-021-3492-5.

Ji-hao MO, Han-kun XIE, Ye-mian ZHOU, et al. Inokosterone Is a Potential Drug Target of Estrogen Receptor 1 in Rheumatoid Arthritis Patients:Analysis from Active Ingredient of Cyathula Officinalis[J]. Chinese Journal of Integrative Medicine, 2021,27(10):767-773. DOI： 10.1007/s11655-021-3492-5.

摘要

Abstract

Objective:

To elucidate the active compounds and the molecular mechanism of

Cyathula Officinalis

as a drug treatment for rheumatoid arthritis (RA).

Methods:

The target genes of active ingredients from

Cyathula Officinalis

were obtained from bioinformatics analysis tool for the molecular mechanism of traditional Chinese medicine. The protein-protein interaction between the target genes were analyzed using STRING and Genemania. The transcriptome of RA patients compared to healthy people (GSE121894) were analyzed using R program package Limma. The relative expression of the target genes was obtained from the RNA-seq datasets. The molecular docking analyses were processed based on the molecular model of estrogen receptor 1 (ESR1) binding with estradiol (PDB ID:1A52). The binding details were analyzed by SYBYL.

Results:

Inokosterone

ecdysterone

and cyaterone were the 3 active ingredients from

Cyathula Officinalis

that bind to target genes. Of all the significantly changed genes from RA patients

ESR1

ADORA1

and ANXA1 were significantly increased in mRNA samples of RA patients.

Conclusion:

ESR1

the transcription factor that binds inokosterone in the molecular binding analysis

is the target protein of

Cyathula Officinalis

关键词

Keywords

rheumatoid arthritisestrogen receptor 1inokosteroneCyathula OfficinalisChinese medicine

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Inokosterone Is a Potential Drug Target of Estrogen Receptor 1 in Rheumatoid Arthritis Patients:Analysis from Active Ingredient of Cyathula Officinalis

Inokosterone Is a Potential Drug Target of Estrogen Receptor 1 in Rheumatoid Arthritis Patients:Analysis from Active Ingredient of Cyathula Officinalis

DOI：10.1007/s11655-021-3492-5

摘要

Abstract

关键词

Keywords

references