针灸抑制TGF-β1/Smad3/Snail通路诱导的克罗恩病患者肠上皮间质转化:一项临床试验研究

Sen GUO; Jing ZHOU; Liang ZHANG; Chun-hui BAO; Ji-meng ZHAO; Yan-ling GAO; Pin WU; Zhi-jun WENG; Yin SHI

doi:10.1007/s11655-022-2888-1

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针灸抑制TGF-β1/Smad3/Snail通路诱导的克罗恩病患者肠上皮间质转化:一项临床试验研究

Acupuncture Research | Updated：2022-09-08

- 针灸抑制TGF-β1/Smad3/Snail通路诱导的克罗恩病患者肠上皮间质转化:一项临床试验研究
- Acupuncture and Moxibustion Inhibited Intestinal Epithelial-Mesenchymal Transition in Patients with Crohn's Disease Induced by TGF-β1/Smad3/Snail Pathway: A Clinical Trial Study
- Chinese Journal of Integrative Medicine 2022年28卷第9期页码：823-832
- Affiliations：
  
  1.Graduate School, Shanghai University of Traditional Chinese Medicine, Shanghai (201203), China
  2.Department of Acupuncture and Moxibustion, Shanghai Traditional Chinese Medicine Integrated Hospital, Shanghai University of Traditional Chinese Medicine,Shanghai (200082), China
  3.Department of Acupuncture and Moxibustion, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai (200437), China
  4.Key Laboratory of Acupuncture and Immunological Effects, Shanghai Research Institute of Acupuncture and Meridian, Shanghai (200030),China
  5.Outpatient Department, Shanghai Research Institute of Acupuncture and Meridian, Shanghai (200030), China
- Author bio：
  
  Prof. SHI Yin, E-mail: flysy0636@163.com
- Funds：
  
  the National Natural Science Foundation of China(81674069;81473757)
- DOI：10.1007/s11655-022-2888-1
  中图分类号：
- 纸质出版日期：2022-09，
  
  网络出版日期：2022-04-13，
  
  录用日期：2021-09-05
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Sen GUO, Jing ZHOU, Liang ZHANG, 等. 针灸抑制TGF-β1/Smad3/Snail通路诱导的克罗恩病患者肠上皮间质转化:一项临床试验研究[J]. Chinese Journal of Integrative Medicine, 2022,28(9):823-832.

Sen GUO, Jing ZHOU, Liang ZHANG, et al. Acupuncture and Moxibustion Inhibited Intestinal Epithelial-Mesenchymal Transition in Patients with Crohn's Disease Induced by TGF-β1/Smad3/Snail Pathway: A Clinical Trial Study[J]. Chinese Journal of Integrative Medicine, 2022,28(9):823-832.
Sen GUO, Jing ZHOU, Liang ZHANG, 等. 针灸抑制TGF-β1/Smad3/Snail通路诱导的克罗恩病患者肠上皮间质转化:一项临床试验研究[J]. Chinese Journal of Integrative Medicine, 2022,28(9):823-832. DOI： 10.1007/s11655-022-2888-1.

Sen GUO, Jing ZHOU, Liang ZHANG, et al. Acupuncture and Moxibustion Inhibited Intestinal Epithelial-Mesenchymal Transition in Patients with Crohn's Disease Induced by TGF-β1/Smad3/Snail Pathway: A Clinical Trial Study[J]. Chinese Journal of Integrative Medicine, 2022,28(9):823-832. DOI： 10.1007/s11655-022-2888-1.

摘要

目的:

探究针灸疗法是否通过影响转化生长因子β1 (TGF-β1) /Smad3/Snail通路阻抑克罗恩病上皮间质转化.

方法:

通过随机数字表将63例轻、中度CD患者随机分为观察组 (31例) 和对照组 (32例)

观察组接受43℃灸结合针刺治疗

对照组采用37℃灸结合假针刺治疗

连续治疗12周. 克罗恩病活动指数 (CDAI) 用于评估疾病活动度. 苏木精-伊红采用染色和透射电镜观察细胞形态和超微结构变化. 免疫组织化学技术检测肠粘膜组织中转化生长因子β1 (TGF-β1)、TβR1、TβR2、Smad3、Snail、E-钙粘蛋白和纤维连接蛋白的表达.

结果:

观察组CDAI评分下降、形态学和超微结构改善更为明显. 观察组TGF-β1、TβR2、Smad3、Snail治疗后的表达水平均显著低于治疗前 (

0.05或

0.01) . 观察组治疗后TGFβ1、TβR2和Snail的表达水平均显著低于对照组治疗后 (

均

0.05) ; 与对照组治疗后比较

观察组治疗后纤维连接蛋白的表达显著降低

E-钙粘蛋白的表达显著升高 (

均

0.05) .

结论:

43℃灸结合针刺治疗可能通过抑制TGF-β1、TβR2、Smad3和snail的表达水平阻抑TGF-β1/Smad3/Snail通路介导的克罗恩患者的肠上皮间质转化.

Abstract

Objective:

To explore whether acupuncture combined with moxibustion could inhibit epithelial-mesenchymal transition in Crohn's disease by affecting the transforming growth factor β1 (TGF-β1)/Smad3/Snail pathway.

Methods:

Sixty-three patients with Crohn's disease were randomly divided into an observation group (31 cases) receiving moxibustion at 43 ℃ combined with acupuncture

and a control group (32 cases) receiving moxibustion at 37 ℃ combined with sham acupuncture using a random number table. Patients were treated for 12 weeks. Crohn's Disease Activity Index (CDAI) was used to evaluate disease activity. Hematoxylin-eosin staining and transmission electron microscopy were utilized to observe the morphological and ultrastructural changes. Immunohistochemistry was used to detect the expression of transforming growth factor β1 (TGF-β1)

TβR1

TβR2

Smad3

Snail

E-cadherin and fibronectin in intestinal mucosal tissues.

Results:

The decrease of the CDAI score

morphological and ultrastructural changes were more significant in observation group. The expression levels of TGF-β1

TβR2

Smad3

and Snail in the observation group were significantly lower than those before the treatment (

0.05 or

0.01). After treatment

the expression levels of TGF-β1

TβR2

and Snail in the observation group were significantly lower than those in the control group (all

0.05); compared with the control group

the expression of fibronectin in the observation group was significantly decreased

and the expression of E-cadherin was significantly increased (all

0.05).

Conclusions:

Moxibustion at 43 ℃ combined with acupuncture may suppress TGF-β1/Smad3/Snail pathway-mediated epithelial-mesenchymal transition of intestinal epithelial cells in Crohn's disease patients by inhibiting the expression levels of TGF-β1

TβR2

Smad3

and Snail. (Registration No. ChiCTR-IIR-16007751)

关键词

克罗恩病艾灸针刺转化生长因子-β1/Smad3/Snail肠上皮-间质转化通路

Keywords

Crohn's diseasemoxibustionacupuncturetransforming growth factor β1/Smad3/Snail pathwayintestinal epithelial-mesenchymal transition

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