白细胞介素-1β/胰岛素样生长因子1介导电针改善原发性高血压所致心肌纤维化的机制研究

XIN Juan-juan; GAO Jun-hong; LIU Qun; ZHAO Yu-xue; ZHOU Chen; YU Xiao-chun

doi:10.1007/s11655-022-2897-0

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白细胞介素-1β/胰岛素样生长因子1介导电针改善原发性高血压所致心肌纤维化的机制研究

Acupuncture Research | Updated：2023-02-01

- 白细胞介素-1β/胰岛素样生长因子1介导电针改善原发性高血压所致心肌纤维化的机制研究
- Involvement of Interleukin-1β/Insulin-Like Growth Factor 1 in Ameliorating Effects of Electroacupuncture on Myocardial Fibrosis Induced by Essential Hypertension
- 中国结合医学杂志（英文版） 2023年29卷第2期页码：162-169
- Affiliations：
  
  Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing (100700), China
- Author bio：
  
  Prof. YU Xiao-chun, E-mail: yuxc@sina.com
- Funds：
  
  the National Natural Science Foundation of China(81804211;81774439);China Academy of Chinese Medical Sciences Foundation(ZZ13-YQ-065)
- DOI：10.1007/s11655-022-2897-0
  中图分类号：
- 纸质出版日期：2023-02，
  
  网络出版日期：2022-07-15，
  
  录用日期：2022-03-21
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白细胞介素-1β/胰岛素样生长因子1介导电针改善原发性高血压所致心肌纤维化的机制研究[J]. 中国结合医学杂志（英文版）, 2023,29(2):162-169.

XIN Juan-juan, GAO Jun-hong, LIU Qun, et al. Involvement of Interleukin-1β/Insulin-Like Growth Factor 1 in Ameliorating Effects of Electroacupuncture on Myocardial Fibrosis Induced by Essential Hypertension[J]. Chinese Journal of Integrative Medicine, 2023,29(2):162-169.
白细胞介素-1β/胰岛素样生长因子1介导电针改善原发性高血压所致心肌纤维化的机制研究[J]. 中国结合医学杂志（英文版）, 2023,29(2):162-169. DOI： 10.1007/s11655-022-2897-0.

XIN Juan-juan, GAO Jun-hong, LIU Qun, et al. Involvement of Interleukin-1β/Insulin-Like Growth Factor 1 in Ameliorating Effects of Electroacupuncture on Myocardial Fibrosis Induced by Essential Hypertension[J]. Chinese Journal of Integrative Medicine, 2023,29(2):162-169. DOI： 10.1007/s11655-022-2897-0.

摘要

目的:

观察电针 "内关" 对自发性高血压大鼠心肌纤维化的影响

并初步探究白细胞介素1β (IL-1β) 、胰岛素样生长因子1 (IGF-1) 和转化生长因子β1 (TGF-β1) 的介导作用.

方法:

选用12周龄雄性Wistar Kyoto (WKY) 大鼠9只为正常组; 另选12周龄自发性高血压大鼠 (SHR) 27只

随机分为SHR组、SHR+EA组和SHR+Sham组

每组9只. 其中

电针组选取 "内关" 进行干预 (2 Hz/15 Hz

1 mA

持续30 min)

每日1次

共8周. 假电针组仅于 "内关" 浅刺

不予电刺激. 电针干预结束后

采用形态学

生化检测等方法分别检测心肌包膜中I型胶原 (Col I) 、Ⅲ型胶原 (Col Ⅲ) 和IGF-1、1L-1β、TGF-β1、基质金属蛋白酶 (MMP) -2和MMP-9水平的组织病理学变化.

结果:

与正常组比较

模型组大鼠反应心肌纤维化的Col I和Col Ⅲ的平均荧光强度升高 (

0.01) 与模型组比较

电针干预8周后Col I和Col Ⅲ的平均荧光强度均降低 (

0.01) ; 与正常组比较

模型组大鼠血清及心肌组织IGF-1、1L-1β、TGF-β1升高 (

0.05)

而心肌组织基质金属蛋白酶 (MMP) -9 mRNA表达降低 (

0.05)

电针干预可明显逆转上述指标的异常 (

0.05

0.01) . 此外

心肌组织及血清中IL-1β、IGF-1、TGF-β1的含量分别与收缩压和羟脯氨酸呈正相关 (

0.01) .

结论:

电针干预可能是通过调控1L-1β/IGF-1-TGF-β1-MMP9通路实现对高血压性心肌纤维化的缓解作用.

Abstract

Objective:

To investigate the effect of electroacupuncture (EA) at Neiguan (PC 6) on myocardial fibrosis in spontaneously hypertensive rats (SHRs)

and to explore the contribution of interleukin-1β (IL-1β)

insulin-like growth factor 1 (IGF-1)

and transforming growth factor β1 (TGF-β1) to the effects.

Methods:

Nine 12-weeks-old Wistar Kyoto (WKY) male rats were employed as the normal group. Twenty-seven SHRs were equally randomized into SHR

SHR+EA

and SHR + sham groups. EA was applied at bilateral PC 6 once a day 30 min per day in 8 consecutive weeks. After 8-weeks EA treatment at PC 6

histopathologic changes of collagen type Ⅰ (Col Ⅰ)

collagen type Ⅲ (Col Ⅲ) and the levels of IGF-1

1L-1β

TGF-β1

matrix metalloproteinase (MMP)-2 and MMP-9 were examined in myocardial tissure respectively.

Results:

After 8-weeks EA treatment at PC 6

the enhanced myocardial fibrosis in SHRs were characterized by the increased mean fluorescence intensity of Col Ⅰ and Col Ⅲ in myocardium tissue (

0.01). All these abnormal alterations above in SHR + EA group was significantly lower compared with the SHR group (

0.01). Meanwhile

the increased levels of IL-1β

IGF-1

TGF-β1 in serum or myocardial tissue of SHRs

diminished MMP 9 mRNA expression in SHRs were also markedly inhibited after 8 weeks of EA treatment (

0.05 or

0.01). Furthermore

the contents of IL-1β

IGF-1

TGF-β1 in myocardial tissue were positively correlated with the systolic blood pressure and hydroxyproline respectively (

0.01).

Conclusion:

EA at bilateral PC 6 could ameliorate cardiac fibrosis in SHRs

which might be mediated by regulation of 1L-1β/IGF-1-TGF-β1-MMP9 pathway.

关键词

电针原发性高血压心肌纤维化白细胞介素-1β胰岛素样生长因子1

Keywords

electroacupuncturespontaneously hypertensive ratsmyocardial fibrosisinterleukin-1βinsulin-like growth factor 1

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