Effects of Buyang Huanwu Decoction on Intestinal Barrier, Intestinal Flora, and Trimethylamine Oxide in Rats with Heart Failure

WENG Jie-qiong; LI Jie-bai; YUAN Meng-fei; YAO Ting-ting; ZHANG Jing-fang; ZENG Yuan-yuan; ZHAO Jing; LI Ying; XU Ke; SHEN Xiao-xu

doi:10.1007/s11655-022-2898-z

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Effects of Buyang Huanwu Decoction on Intestinal Barrier, Intestinal Flora, and Trimethylamine Oxide in Rats with Heart Failure

Original Article | Updated：2023-02-01

- Effects of Buyang Huanwu Decoction on Intestinal Barrier, Intestinal Flora, and Trimethylamine Oxide in Rats with Heart Failure
- Effects of Buyang Huanwu Decoction on Intestinal Barrier, Intestinal Flora, and Trimethylamine Oxide in Rats with Heart Failure
- 中国结合医学杂志（英文版） 2023年29卷第2期页码：155-161
- Affiliations：
  
  1.Department of Cardiology, Dongzhimen Hospital of Beijing University of Chinese Medicine, Beijing (100700), China
  2.Department of Nuclear Medicine, Dongzhimen Hospital of Beijing University of Chinese Medicine, Beijing (100700), China
  3.Peking University Sixth Hospital, Beijing (100700), China
- Author bio：
  
  Prof. SHEN Xiao-xu, E-mail: doctorshen_paper@sina.com.
- Funds：
  
  China Key R&D Program of the Ministry of Science and Technology(2017YFC1700206)
- DOI：10.1007/s11655-022-2898-z
  中图分类号：
- 纸质出版日期：2023-02，
  
  网络出版日期：2022-11-12，
  
  录用日期：2022-05-13
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Effects of Buyang Huanwu Decoction on Intestinal Barrier, Intestinal Flora, and Trimethylamine Oxide in Rats with Heart Failure[J]. 中国结合医学杂志（英文版）, 2023,29(2):155-161.

WENG Jie-qiong, LI Jie-bai, YUAN Meng-fei, et al. Effects of Buyang Huanwu Decoction on Intestinal Barrier, Intestinal Flora, and Trimethylamine Oxide in Rats with Heart Failure[J]. Chinese Journal of Integrative Medicine, 2023,29(2):155-161.
Effects of Buyang Huanwu Decoction on Intestinal Barrier, Intestinal Flora, and Trimethylamine Oxide in Rats with Heart Failure[J]. 中国结合医学杂志（英文版）, 2023,29(2):155-161. DOI： 10.1007/s11655-022-2898-z.

WENG Jie-qiong, LI Jie-bai, YUAN Meng-fei, et al. Effects of Buyang Huanwu Decoction on Intestinal Barrier, Intestinal Flora, and Trimethylamine Oxide in Rats with Heart Failure[J]. Chinese Journal of Integrative Medicine, 2023,29(2):155-161. DOI： 10.1007/s11655-022-2898-z.

摘要

Abstract

Objective:

To explore the mechanisms of Buyang Huanwu Decoction (BYHWD) modulating the gut microbiome and trimethylamine oxide (TAMO) to exert cardioprotective effects.

Methods:

Ligation of the left anterior descending coronary artery was performed in rats to induce heart failure (HF). Except for the sham-operation group (

=10)

36 operation-induced models were randomized into 3 groups using a random number table (

=12 in each group): the model group

the BYHWD group (15.02 g/kg BYHWD)

and the positive group (4.99 g/kg metoprolol succinate). After 4-week treatment (once daily by gavage)

echocardiography was applied to evaluate the cardiac function and the Tei index (the ratio of ventricular isovolumic contraction time (IVCT) and isovolumic diastolic time (IVRT) to ejection time (ET)) was calculated; hematoxylin-eosin (HE) staining was observed to characterize the pathology of the myocardium and small intestinal villi. D-lactic acid was detected by an enzyme-linked immunosorbent assay (ELISA). Expressions of occludin

claudin-1

and zonula occludens (ZO-1) were detected by Western blot. 16S ribosomal ribonucleic acid (16S rRNA) sequencing was used to explore the changes in the intestinal flora. TMAO was detected via liquid chromatography-tandem mass spectrometry (LC-MS/MS).

Results:

In the echocardiography

the Tei index was considerably lower in the positive and BYHWD groups compared with the model group (

0.05). Besides

BYHWD improved the pathology of myocardium and small intestine of HF rats and lowered the D-lactic acid content in the serum

when compared with the model group (

0.05). BYHWD also improved the expression of occludin and claudin-1 (

0.05); in the gut microbiota analysis

BYHWD slowed down modifications in the structure distribution of gut microbiota and regulated the diversity of intestinal flora in HF rats. The content of TMAO in the serum was significantly lowered by BYWHT compared with the model group (

0.05).

Conclusion:

BYHWD may delay progression of HF by enhancing the intestinal barrier structure

and regulating intestinal flora and TAMO.

关键词

Keywords

Buyang Huanwu Decoctionheart failureintestinal floratrimethylamine oxide

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