Protective Effect and Mechanism of Kuntai Capsule on Angiotensin Ⅱ-Induced Hypertension in Ovariectomized Rats

GE Xiao-fen; LI Sha-sha; LIU Yan-hua; LU Mei-qiu; SU Hui-na; YANG Xin; SUN Xiao-wan

doi:10.1007/s11655-022-3514-3

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Protective Effect and Mechanism of Kuntai Capsule on Angiotensin Ⅱ-Induced Hypertension in Ovariectomized Rats

Original Article | Updated：2023-05-25

- Protective Effect and Mechanism of Kuntai Capsule on Angiotensin Ⅱ-Induced Hypertension in Ovariectomized Rats
- Protective Effect and Mechanism of Kuntai Capsule on Angiotensin Ⅱ-Induced Hypertension in Ovariectomized Rats
- 中国结合医学杂志（英文版） 2023年29卷第6期页码：526-533
- Affiliations：
  
  1.Department of Obstetrics and Gynecology, Peking University People's Hospital, Beijing (100044), China
  2.Department of Obstetrics and Gynecology, Dongying People's Hospital, Dongying, Shandong Province (257000), China
- Author bio：
  
  Prof. YANG Xin, E-mail: xinyang_2003@sina.com
- Funds：
  
  2020 Bethune Excellent Talent Training Program for Young and Middle-Aged Doctors
- DOI：10.1007/s11655-022-3514-3
  中图分类号：
- 纸质出版日期：2023-06，
  
  网络出版日期：2022-11-03，
  
  录用日期：2020-07-13
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Protective Effect and Mechanism of Kuntai Capsule on Angiotensin Ⅱ-Induced Hypertension in Ovariectomized Rats[J]. 中国结合医学杂志（英文版）, 2023,29(6):526-533.

GE Xiao-fen, LI Sha-sha, LIU Yan-hua, et al. Protective Effect and Mechanism of Kuntai Capsule on Angiotensin Ⅱ-Induced Hypertension in Ovariectomized Rats[J]. Chinese Journal of Integrative Medicine, 2023,29(6):526-533.
Protective Effect and Mechanism of Kuntai Capsule on Angiotensin Ⅱ-Induced Hypertension in Ovariectomized Rats[J]. 中国结合医学杂志（英文版）, 2023,29(6):526-533. DOI： 10.1007/s11655-022-3514-3.

GE Xiao-fen, LI Sha-sha, LIU Yan-hua, et al. Protective Effect and Mechanism of Kuntai Capsule on Angiotensin Ⅱ-Induced Hypertension in Ovariectomized Rats[J]. Chinese Journal of Integrative Medicine, 2023,29(6):526-533. DOI： 10.1007/s11655-022-3514-3.

摘要

Abstract

Objective:

To explore the protective effect and mechanism of Kuntai (KT) Capsule on angiotensin Ⅱ (AngⅡ)-induced hypertension in ovariectomized (OVX) rats.

Methods:

Fifty-four rats were randomly divided into 6 groups according to a random number table

9 in each group: control

OVX sham+AngⅡ

OVX

OVX+AngⅡ

OVX+AngⅡ+E

and OVX+AngⅡ+KT. OVX rats model was constructed by retroperitoneal bilateral ovariectomy. After 4 weeks of pretreatment with KT Capsule [0.8 g/(kg•d) and 17-β-estradiol (E

1.2 mg/(kg•d)] respectively

AngⅡ was injected into a micro-osmotic pump with a syringe to establish a hypertensive rat model. Blood pressure of rat tail artery was measured in a wake state of rats using a non-invasive sphygmomanometer. Blood pressure changes were compared between the intervention groups (OVX+AngⅡ+KT

OVX+AngⅡ+E

) and the negative control group (OVX+AngⅡ). Serum malondialdehyde (MDA) level and superoxide dismutase (SOD) activity were detected respectively. The expressions of oxidative stress-related protein superoxide dismutase2 (SOD2) and anti-thioredoxin (TRX)

autophagy marker protein [beclin1

light chain (LC) 3Ⅱ/Ⅰ ratio and autophagy canonical pathway protein phosphatidylinositol 3-kinase (PI3K)/serine/threonine kinase (AKT)-mammalian target of rapamycin (mTOR)] were evaluated by Western blotting.

Results:

Compared with the OVX+AngⅡ group

the systolic blood pressure of OVX+AngⅡ+KT group was significantly lowered (

0.05) but not the diastolic blood pressure. Besides

SOD2 and TRX protein levels in mycardial tissues were significantly reduced in the OVX+AngⅡ+KT group compared with the OVX+AngⅡ group (

0.05). Oxidative stress serum markers MDA and SOD were down- and up-regulated in the OVX+AngⅡ+KT group

respectively (

0.05). Compared with OVX+AngⅡ group

the levels of cardiac proteins beclin-1 and LC3Ⅱ/LC3Ⅰ in OVX+AngⅡ+KT group were also up-regulated (

0.05)

and the expression levels of p-PI3K

p-AKT and mTOR protein were down-regulated (

0.05).

Conclusion:

KT could protect blood pressure of AngⅡ-induced OVX rats by inhibiting oxidative stress and up-regulating protective autophagy.

关键词

Keywords

Kuntai CapsuleChinese medicineperimenopausal hypertensionangiotensin Ⅱ

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