Objective:

2

To investigate the anti-inflammatory activity of

Radix Panacis quinquefolii

extract (RPQE) and its therapeutic effects on inflammatory bowel disease (IBD).

Methods:

2

The 72-hour post-fertilization zebrafish was used to generate the local and systematic inflammation models through tail-amputation and lipopolysaccharide (LPS)-induction (100 μg/mL)

respectively. The Tg(zlyz:EGFP) zebrafish was induced with 75 μg/mL 2

4

6-trinitrobenzene sulfonic acid (TNBS) for establishing the IBD model. The tail-amputated

LPS-

and TNBS-induced models were subjected to RPQE (ethanol fraction

10–20 μg/mL) administration for 12 and 24 h

respectively. Anti-inflammatory activity of RPQE was evaluated by detecting migration and aggregation of leukocytes and expression of inflammation-related genes. Meanwhile

TNBS-induced fish were immersed in 0.2% (W/V) calcein for 1.5 h and RPQE for 12 h before photographing to analyze the intestinal efflux efficiency (IEE). Moreover

the expression of inflammation-related genes in these fish was detected by quantitative polymerase chain reaction.

Results:

2

Subject to RPQE administration

the migration and aggregation of leukocytes were significantly alleviated in 3 zebrafish models (

P

<

0.01). Herein

RPQE ameliorated TNBS-induced IBD with respect to a significantly reduced number of leukocytes

improved IEE

and inhibited gene expression of pro-inflammatory factors (

P

<

0.05 or

P

<

0.01).

Conclusion:

2

RPQE exhibited therapeutic effects on IBD by inhibiting inflammation.