自血穴位注射对二硝基氯苯诱导特应性皮炎小鼠Th1/Th2免疫失衡的调节作用

Zhi-wen ZENG; Jin-quan HUANG; Yong CHEN; Xiao YU; Wei ZHU; Dong-shu ZHANG

doi:10.1007/s11655-022-3579-7

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自血穴位注射对二硝基氯苯诱导特应性皮炎小鼠Th1/Th2免疫失衡的调节作用

Original Article | Updated：2022-06-27

- 自血穴位注射对二硝基氯苯诱导特应性皮炎小鼠Th1/Th2免疫失衡的调节作用
- Acupoint Autohemotherapy Attenuates Atopic Dermatitis Lesions by Regulating Th1/Th2 Balance in DNCB-Induced BALB/c Mice
- Chinese Journal of Integrative Medicine 2022年28卷第7期页码：612-619
- Affiliations：
  
  1.School of Traditional Chinese Medicine, Southern Medical University, Guangzhou (510515), China
  2.Department of Traditional Chinese Medicine, Affiliated Dongguan People's Hospital, Southern Medical University, Dongguan, Guangdong Province (523058), China
  3.Department of Rheumatology and Immunology, Shenzhen People's Hospital, The Second Clinical Medical College of Jinan University, Shenzhen, Guangdong Province (518020), China
  4.School of Basic Medical Sciences,Southern Medical University, Guangzhou (510515), China
  5.Guangzhou Center for Disease Control and Prevention, Guangzhou (510515), China
- Author bio：
  
  Prof. ZHANG Dong-shu, E-mail: nyzds@sina.com
- Funds：
  
  the National Natural Science Foundation of China(81774390);the Traditional Chinese Medicine Bureau of Guangdong Province(20201022;20211258)
- DOI：10.1007/s11655-022-3579-7
  中图分类号：
- 纸质出版日期：2022-07-01，
  
  录用日期：2022-03-25
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Zhi-wen ZENG, Jin-quan HUANG, Yong CHEN, 等. 自血穴位注射对二硝基氯苯诱导特应性皮炎小鼠Th1/Th2免疫失衡的调节作用[J]. Chinese Journal of Integrative Medicine, 2022,28(7):612-619.

Zhi-wen ZENG, Jin-quan HUANG, Yong CHEN, et al. Acupoint Autohemotherapy Attenuates Atopic Dermatitis Lesions by Regulating Th1/Th2 Balance in DNCB-Induced BALB/c Mice[J]. Chinese Journal of Integrative Medicine, 2022,28(7):612-619.
Zhi-wen ZENG, Jin-quan HUANG, Yong CHEN, 等. 自血穴位注射对二硝基氯苯诱导特应性皮炎小鼠Th1/Th2免疫失衡的调节作用[J]. Chinese Journal of Integrative Medicine, 2022,28(7):612-619. DOI： 10.1007/s11655-022-3579-7.

Zhi-wen ZENG, Jin-quan HUANG, Yong CHEN, et al. Acupoint Autohemotherapy Attenuates Atopic Dermatitis Lesions by Regulating Th1/Th2 Balance in DNCB-Induced BALB/c Mice[J]. Chinese Journal of Integrative Medicine, 2022,28(7):612-619. DOI： 10.1007/s11655-022-3579-7.

摘要

目的:

评价自血穴位注射疗法 (Acupoint Autohemotherapy

A-AHT) 对1-氯-2

4-二硝基苯 (1-chloro-2

4-dinitrobenzene

DNCB) 诱导的特应性皮炎 (Atopic dermatitis

AD) 小鼠Th1/Th2免疫失衡的调节作用.

方法:

将30只BALB/c小鼠随机分为5组

包括正常对照组 (Normal control

NC) 、AD模型组 (AD) 、自血穴位注射组 (A-AHT) 、自血非穴注射组 (Sham acupoint Autohemotherapy

sA-AHT) 、生理盐水穴位注射组 (Acupoint injection of normal saline

A-NS)

每组6只小鼠. 采用DNCB 致敏小鼠建立实验性AD小鼠模型. 实验第8天起

除NC组和AD组外

其余各组小鼠隔日干预一次

共28天. A-AHT组小鼠抽取自体全血 (autologous whole blood

AWB) 注入双侧足三里 (ST 36) 、曲池 (LI 11) 穴

每穴0.05 mL; sA-AHT组小鼠则将AWB注入对应的非穴 (双侧足三里、曲池穴外旁开5 mm) . A-NS组小鼠于双侧足三里、曲池穴注射生理盐水0.05mL. 采用特应性皮炎严重程度评分 (SCORAD)评价小鼠背部皮损严重程度

每周一次; 运用酶联免疫吸附试验 (ELISA) 检测小鼠血清总免疫球蛋白E (Ig-E) 、干扰素γ (IFN-γ) 和白细胞介素4 (IL-4) 表达水平

流式细胞术 (FCM) 分析小鼠脾细胞Th1/Th2比值

免疫组织化学法 (IHC) 评估皮损组织T-bet和GATA-3的表达.

结果:

与AD组相比

A-AHT和sA-AHT两治疗组小鼠SCORAD评分与血清IgE水平下降 (

0.05或

0.01) ; A-AHT、sA-AHT和A-NS三组小鼠血清IL-4表达下降

IFN-γ/IL-4比值上升 (

0.05或

0.01) ; 而只有A-AHT治疗能够调节AD小鼠Th1/Th2失衡

增加其转录因子T-bet表达并上调T-bet/GATA3比值 (

0.05) .

结论:

自血穴位注射可以通过调节Th1/Th2免疫失衡改善AD小鼠症状.

Abstract

Objective:

To evaluate the therapeutic effects of acupoint autohemotherapy (A-AHT) on 1-chloro- 2

4-dinitrobenzene (DNCB)-induced atopic dermatitis (AD) in mice focusing on regulating T helper 1/T helper 2 (Th1/Th2) immune responses.

Methods:

Thirty BALB/c mice were divided into 5 groups by a random number table

including normal control (NC)

AD model (AD)

A-AHT

sham A-AHT (sA-AHT)

and acupoint injection of normal saline (A-NS) groups

6 mice per group. Mice were challenged by DNCB for the establishment of experimental AD model. On the 8th day

except for the NC and AD groups

the mice in the other groups received management once every other day for a total of 28 days. For the A-AHT and sA-AHT groups

0.05 mL of autologous whole blood (AWB) was injected into bilateral Zusanli (ST 36) and Quchi (LI 11) and sham-acupoints (5 mm lateral to ST 36 and LI 11)

respectively. The A-NS group was administrated with 0.05 mL of normal saline by acupoint injection into ST 36 and LI 11. Dermatitis severity for dorsal skin of mice was determined using the Severity Scoring of Atopic Dermatitis (SCORAD) every week. The total immunoglobulin E (IgE)

interleukin-4 (IL-4)

and interferon-gamma (IFN-γ) cytokine levels in serum were examined by enzyme-linked immunosorbent assay (ELISA). Spleen Th1/Th2 expression were analyzed via flow cytometry and immunohistochemical assay was used to detect T-box expressed in T cell (T-bet) and GATA-binding protein 3 (GATA3) expressions in skin lesions of mice.

Results:

Compared with the AD group

both A-AHT and sA-AHT reduced the SCORAD index and serum IgE level (

0.05 or

0.01); A-AHT

sA-AHT and A-NS down-regulated serum IL-4 level and upregulated IFN-γ/IL-4 ratio (

0.05 or

0.01); A-AHT regulated the Th1/Th2 shift specifically and increased the related transcription factors such as T-bet expression and T-bet/GATA3 ratio (

0.05).

Conclusion:

A-AHT showed significant effectiveness on the AD model mice

through regulating Th1/Th2 immune responses.

关键词

Keywords

acupoint autohemotherapyatopic dermatitisTh1/Th2immunoglobulin E

references

Rizk P, Rodenas M, De Benedetto A. Allergen immunotherapy and atopic dermatitis: the good, the bad,and the unknown. Curr Allergy Asthma Rep 2019;19:57.

Bieber T. Atopic dermatitis. Ann Dermatol 2010;22:125-137.

Malik K, Heitmiller KD, Czarnowicki T. An update on the pathophysiology of atopic dermatitis. Dermatol Clin 2017;35:317-326.

Akdis CA, Akdis M, Bieber T, Bindslev-Jensen C, Boguniewicz M, Eigenmann P, et al. Diagnosis and treatment of atopic dermatitis in children and adults: European Academy of Allergology and Clinical Immunology/American Academy of Allergy, Asthma and Immunology/PRACTALL Consensus Report. J Allergy Clin Immunol 2006;118:152-169.

Ravaut P. Essay on autohemotherapy in some dermatoses.Ann Dermatol Syphiligr 1913;4:292-296.

Behl PN, ed. Practice of dermatology. 7th ed. New Delhi:Oxford Blackwell Scientific Publications;1990:76.

Pittler MH, Armstrong NC, Cox A, Collier PM, Hart A,Ernst E. Randomized, double-blind, placebo-controlled trial of autologous blood therapy for atopic dermatitis. Br J Dermatol 2003;148:307-313.

Huang C, Chen C, Zhou R, Liang Y, Zhang J, Hong H,et al. A systematic review and meta-analysis of acupoint autohemotherapy and Western medicine therapy in treating chronic obstructive pulmonary disease. Complement Ther Clin Pract 2021;43:101336.

He CB, Li P, Luo NY. Clinical study on chronic eczema treated by interventional autohemotherapy of acupoints. J Pract Tradit Chin Med (Chin) 2011;27:372-373.

Zhao B, Chen Y, Liao SM, Zheng JY, Yan SH, Zhang DS. Successfully treated recalcitrant atopic eczema with acupoint autohemotherapy: a case report and hypothesized mechanism of the therapy. J Integr Med 2022;20:182-186.

Li CC, Yin YH, Yang HS, Wang ZY, Wang SJ. A systematic review of curative effect and safety of autohemotherapy for chronic eczema. Chin J Basic Med Tradit Chin Med (Chin)2018;24:1597-1599,1606.

Huang JQ, Zeng ZW, Chen Y, Zhang DS. Effect of acupoint autohemotherapy on DNCB-induced atopic dermatitis-like symptoms in BALB/c mice. Chin J Integr Tradit West Med(Chin) 2021 Dec 2. [Epub ahead of print]

Demers G, Griffin G, De Vroey G, Haywood JR, Zurlo J,Bédard M. Animal research. Harmonization of animal care and use guidance. Science 2006;312:700-701.

Lee Y, Choi HK, N'deh KPU, Choi YJ, Fan M, Kim EK,et al. Inhibitory effect of Centella asiatica extract on DNCB-induced atopic dermatitis in HaCaT cells and BALB/c mice.Nutrients 2020;12:411.

Yang HR, Lee H, Kim JH, Hong IH, Hwang DH, Rho IR,et al. Therapeutic effect of Rumex japonicus Houtt. on DNCB-induced atopic dermatitis-like skin lesions in BALB/c mice and human keratinocyte HaCaT cells. Nutrients 2019;11:573.

Park JY, Park HJ, Choi YY, Kim MH, Kim SN,Yang WM. Effects of acupuncture on 1-chloro-2,4-dinitrochlorobenzene-induced atopic dermatitis. Evid Based Complement Alternat Med 2013;2013:831539.

Wang Z, Yi T, Long M, Ding F, Ouyang L, Chen Z.Involvement of the negative feedback of IL-33 signaling in the anti-inflammatory effect of electro-acupuncture on allergic contact dermatitis via targeting microRNA-155 in mast cells. Inflammation 2018;41:859-869.

Meng X, Guo X, Zhang J, Moriya J, Kobayashi J,Yamaguchi R, et al. Acupuncture on ST36, CV4 and KI1 suppresses the progression of methionine- and choline-deficient diet-induced nonalcoholic fatty liver disease in mice. Metabolites 2019;9:299.

Tsai KS, Chen YH, Chen HY, Shen EY, Lee YC, Shen JL,et al. Antipruritic effect of cold stimulation at the Quchi acupoint(LI11) in mice. BMC Complement Altern Med 2014;14:341.

Severity scoring of atopic dermatitis: the SCORAD index.Consensus Report of the European Task Force on Atopic Dermatitis. Dermatology 1993;186:23-31.

Lu X, Cui J, Cui L, Luo Q, Cao Q, Yuan W, et al. The effects of human umbilical cord-derived mesenchymal stem cell transplantation on endometrial receptivity are associated with Th1/Th2 balance change and uNK cell expression of uterine in autoimmune premature ovarian failure mice.Stem Cell Res Ther 2019;10:214.

de Kouchkovsky DA, Ghosh S, Rothlin CV. Negative regulation of type 2 immunity. Trends Immunol 2017;38:154-167.

Osinka K, Dumycz K, Kwiek B, Feleszko W. Novel therapeutic approaches to atopic dermatitis. Arch Immunol Ther Exp (Warsz) 2018;66:171-181.

Lazarevic V, Glimcher LH, Lord GM. T-bet: a bridge between innate and adaptive immunity. Nat Rev Immunol 2013;13:777-789.

Chen Y, Liu YP, Ban SF, Tang Y, Qiu FJ, Xiao CH. What happened in the acupoint area of mouse after blood injection? J South Med Univ (Chin) 2020;40:1225-1229.

Nahm DH, Ye YM, Shin YS, Park HS, Kim ME, Kwon B, et al. Efficacy, safety, and immunomodulatory effect of the intramuscular administration of autologous total immunoglobulin G for atopic dermatitis: a randomized clinical trial. Allergy Asthma Immunol Res 2020;12:949-963.

Huang R, Li X, Xu S, Li D, Yan P, Liu B, et al. Acupoint injection treatment for primary osteoporosis: a systematic review and meta-analysis of randomized controlled trials.Ann Palliat Med 2019;8:586-595.

Jin H, Xu Y, Shi F, Hu S. Vaccination at different anatomic sites induces different levels of the immune responses. Res Vet Sci 2019;122:50-55.

Liu S, Wang Z, Su Y, Qi L, Yang W, Fu M, et al. A neuroanatomical basis for electroacupuncture to drive the vagal-adrenal axis. Nature 2021;598:641-645.

Kim SK, Bae H. Acupuncture and immune modulation.Auton Neurosci 2010;157:38-41.

Qin XY, Zhan XQ, Han JT, Ye L. Effect of acupuncture at Quchi point on erythrocyte immunity in mice. Modern J Integr Tradit Chin West Med (Chin) 2000;9:283-284.

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