Grape Seed Extract Attenuates Demyelination in Experimental Autoimmune Encephalomyelitis Mice by Inhibiting Inflammatory Response of Immune Cells

WANG Qing; CHEN Yang-yang; YANG Zhi-chao; YUAN Hai-jun; DONG Yi-wei; MIAO Qiang; LI Yan-qing; WANG Jing; YU Jie-zhong; XIAO Bao-guo; MA Cun-gen

doi:10.1007/s11655-022-3587-7

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Grape Seed Extract Attenuates Demyelination in Experimental Autoimmune Encephalomyelitis Mice by Inhibiting Inflammatory Response of Immune Cells

Original Article | Updated：2023-04-27

- Grape Seed Extract Attenuates Demyelination in Experimental Autoimmune Encephalomyelitis Mice by Inhibiting Inflammatory Response of Immune Cells
- Grape Seed Extract Attenuates Demyelination in Experimental Autoimmune Encephalomyelitis Mice by Inhibiting Inflammatory Response of Immune Cells
- 中国结合医学杂志（英文版） 2023年29卷第5期页码：394-404
- Affiliations：
  
  1.Research Center of Neurobiology, the Key Research Laboratory of Benefiting Qi for Acting Blood Circulation Method to Treat Multiple Sclerosis of State Administration of Traditional Chinese Medicine, Shanxi University of Chinese Medicine, Jinzhong, Shanxi Province (030619), China
  2.Department of Neurology, the First Affiliated Hospital, Shanxi Medical University, Taiyuan (030001), China
  3.Institute of Brain Science, Shanxi Key Laboratory of Inflammatory Neurodegenerative Diseases, Shanxi Datong University, Datong, Shanxi Province (037009), China
  4.Department of Neurology, Datong Fifth People's Hospital, Datong, Shanxi Province (037009), China
  5.Institute of Neurology, Huashan Hospital, Institutes of Brain Science and State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai (200000), China
- Author bio：
  
  Prof. MA Cun-gen, E-mail: macungen@sxtcm.edu.cn
- Funds：
  
  the National Natural Science Foundation of China(81903596);Science and Technology Innovation Project of Shanxi Colleges(2019L0728);Leading Team of Medical Science and Technology of Shanxi Province(2020TD05);Funds for Construction of Key Disciplines from Shanxi University of Chinese Medicine(030200117);Cultivation Project of Shanxi University of Chinese Medicine(2019PY130;2020PY-JC-14)
- DOI：10.1007/s11655-022-3587-7
  中图分类号：
- 纸质出版日期：2023-05，
  
  网络出版日期：2023-01-6，
  
  录用日期：2022-05-29
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Grape Seed Extract Attenuates Demyelination in Experimental Autoimmune Encephalomyelitis Mice by Inhibiting Inflammatory Response of Immune Cells[J]. 中国结合医学杂志（英文版）, 2023,29(5):394-404.

WANG Qing, CHEN Yang-yang, YANG Zhi-chao, et al. Grape Seed Extract Attenuates Demyelination in Experimental Autoimmune Encephalomyelitis Mice by Inhibiting Inflammatory Response of Immune Cells[J]. Chinese Journal of Integrative Medicine, 2023,29(5):394-404.
Grape Seed Extract Attenuates Demyelination in Experimental Autoimmune Encephalomyelitis Mice by Inhibiting Inflammatory Response of Immune Cells[J]. 中国结合医学杂志（英文版）, 2023,29(5):394-404. DOI： 10.1007/s11655-022-3587-7.

WANG Qing, CHEN Yang-yang, YANG Zhi-chao, et al. Grape Seed Extract Attenuates Demyelination in Experimental Autoimmune Encephalomyelitis Mice by Inhibiting Inflammatory Response of Immune Cells[J]. Chinese Journal of Integrative Medicine, 2023,29(5):394-404. DOI： 10.1007/s11655-022-3587-7.

摘要

Abstract

Objective:

To examine the anti-inflammatory effect of grape seed extract (GSE) in animal and cellular models and explore its mechanism of action.

Methods:

This study determined the inhibitory effect of GSE on macrophage inflammation and Th1 and Th17 polarization

in vitro

. Based on the

in vitro

results

the effects and mechanisms of GSE on multiple sclerosis (MS)-experimental autoimmune encephalomyelitis (EAE) mice model were further explored. The C57BL/6 mice were intragastrically administered with 50 mg/kg of GSE once a day from the 3rd day to the 27th day after immunization. The activation of microglia

the polarization of Th1 and Th17 and the inflammatory factors such as tumor necrosis factor-α (TNF-α)

interleukin-1β (IL-1β)

IL-6

IL-12

IL-17 and interferon-γ (IFN-γ) secreted by them were detected

in vitro

and

in vivo

by flow cytometry

enzyme linked immunosorbent assay (ELISA)

immunofluorescence staining and Western blot

respectively.

Results:

GSE reduced the secretion of TNF-α

IL-1β and IL-6 in bone marrow-derived macrophages stimulated by lipopolysaccharide (

0.01)

inhibited the secretion of TNF-α

IL-1β

IL-6

IL-12

IL-17 and IFN-γ in spleen cells of EAE mice immunized for 9 days (

0.05 or

0.01)

and reduced the differentiation of Th1 and Th17 mediated by CD3 and CD28 factors (

0.01). GSE significantly improved the clinical symptoms of EAE mice

and inhibited spinal cord demyelination and inflammatory cell infiltration. Peripherally

GSE downregulated the expression of toll-like-receptor 4 (TLR4) and Rho-associated kinase (ROCKⅡ

0.05 or

0.01)

and inhibited the secretion of inflammatory factors (

0.01 or

0.05). In the central nervous system

GSE inhibited the infiltration of CD45

CD11b

and CD45

CD4

cells

and weakened the differentiation of Th1 and Th17 (

0.05). Moreover

it reduced the secretion of inflammatory factors (

0.01)

and prevented the activation of microglia (

0.05).

Conclusion:

GSE hada beneficial effect on the pathogenesis and progression of EAE by inhibiting inflammatory response as a potential drug and strategy for the treatment of MS.

关键词

Keywords

grape seed extractexperimental autoimmune encephalomyelitisdemyelinationinflammatory cellinflammatory factor

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