Effects of Total Saponins from <italic style="font-style: italic">Dioscorea Nipponica</italic> Makino on Monosodium Urate-Induced M1-Polarized Macrophages through Arachidonic Acid Signaling Pathway: An <italic style="font-style: italic">in vitro</italic> Study

ZHOU Qi; SUN Hui-juan; LIU Shu-min

doi:10.1007/s11655-022-3721-6

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Effects of Total Saponins from Dioscorea Nipponica Makino on Monosodium Urate-Induced M1-Polarized Macrophages through Arachidonic Acid Signaling Pathway: An in vitro Study

Original Article | Updated：2023-01-05

- Effects of Total Saponins from Dioscorea Nipponica Makino on Monosodium Urate-Induced M1-Polarized Macrophages through Arachidonic Acid Signaling Pathway: An in vitro Study
- Effects of Total Saponins from Dioscorea Nipponica Makino on Monosodium Urate-Induced M1-Polarized Macrophages through Arachidonic Acid Signaling Pathway: An in vitro Study
- 中国结合医学杂志（英文版） 2023年29卷第1期页码：44-51
- Affiliations：
  
  1.Research Institute of Chinese Medicine, Heilongjiang University of Chinese Medicine, Harbin (150040), China
  2.Graduate School, Heilongjiang University of Chinese Medicine, Harbin (150040), China
  3.Technological Innovation Team of Basic Theory Study Research of Institution of Higher Education in Heilongjiang Province, Heilongjiang University of Chinese Medicine, Harbin (150040), China
- Author bio：
  
  Prof. LIU Shu-min, E-mail: keji-liu@163.com
- Funds：
  
  Joint Guidance Project of Heilongjiang Natural Science Foundation(LH2021H099);Start Fund of Postdoctoral Research in Heilongjiang Province(LBH-Q19188);Outstanding Youth Development Fund of Heilongjiang University of Chinese Medicine(2019JC06);Project of Heilongjiang Administration of Traditional Chinese Medicine(ZHY202094);Graduate Innovative Scientific Research Project of Heilongjiang University of Chinese Medicine(2020yjscx057);Natural Science Foundation of Heilongjiang Province (Key project)(ZD2020H006)
- DOI：10.1007/s11655-022-3721-6
  中图分类号：
- 纸质出版日期：2023-01-01，
  
  网络出版日期：2022-07-12，
  
  录用日期：2021-10-30
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Effects of Total Saponins from Dioscorea Nipponica Makino on Monosodium Urate-Induced M1-Polarized Macrophages through Arachidonic Acid Signaling Pathway: An in vitro Study[J]. 中国结合医学杂志（英文版）, 2023,29(1):44-51.

ZHOU Qi, SUN Hui-juan, LIU Shu-min. Effects of Total Saponins from Dioscorea Nipponica Makino on Monosodium Urate-Induced M1-Polarized Macrophages through Arachidonic Acid Signaling Pathway: An in vitro Study[J]. Chinese Journal of Integrative Medicine, 2023,29(1):44-51.
Effects of Total Saponins from Dioscorea Nipponica Makino on Monosodium Urate-Induced M1-Polarized Macrophages through Arachidonic Acid Signaling Pathway: An in vitro Study[J]. 中国结合医学杂志（英文版）, 2023,29(1):44-51. DOI： 10.1007/s11655-022-3721-6.

ZHOU Qi, SUN Hui-juan, LIU Shu-min. Effects of Total Saponins from Dioscorea Nipponica Makino on Monosodium Urate-Induced M1-Polarized Macrophages through Arachidonic Acid Signaling Pathway: An in vitro Study[J]. Chinese Journal of Integrative Medicine, 2023,29(1):44-51. DOI： 10.1007/s11655-022-3721-6.

摘要

Abstract

Objective:

To investigate and reveal the underlying mechanism of the effect of total saponins from

Dioscoreae nipponica

Makino (TSDN) on the arachidonic acid pathway in monosodium urate (MSU) crystal-induced M1-polarized macrophages.

Methods:

M1 polarization of RAW264.7 cells were induced by 1 μg/mL lipopolysaccharide (LPS). The methylthiazolyldiphenyl-tetrazolium bromide method was then used to screen the concentration of TSDN. MSU (500 μg/mL) was used to induce the gouty arthritis model. Afterwards

10 μg/L TSDN and 8 μmol/L celecoxib

which was used as a positive control

were added to the above LPS and MSU-induced cells for 24 h. The mRNA and protein expressions of cyclooxygenase (COX) 2

5-lipoxygenase (5-LOX)

microsomal prostaglandin E synthase derived eicosanoids (mPGES)-1

leukotriene B (LTB)4

cytochrome P450 (CYP) 4A

and prostaglandin E

(PGE

) were tested by real-time polymerase chain reaction and Western blotting

respectively. The enzyme-linked immunosorbent assay was used to test the contents of M1 markers

including inducible nitric oxid synthase (NOS) 2

CD80

and CD86.

Results:

TSDN inhibited the proliferation of M1 macrophages and decreased both the mRNA and protein expressions of COX2

5-LOX

CYP4A

LTB4

and PGE

(

0.01) while increased the mRNA and protein expression of mPGES-1 (

0.05 or

0.01). TSDN could also significantly decrease the contents of NOS2

CD80

and CD86 (

0.01).

Conclusion:

TSDN has an anti-inflammation effect on gouty arthritis in an

in vitro

model by regulating arachidonic acid signaling pathway.

关键词

Keywords

gouty arthritisDioscorea nipponica Makinoarachidonic acidsignaling pathwayM1/M2 polarization

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Effects of Total Saponins from Dioscorea Nipponica Makino on Monosodium Urate-Induced M1-Polarized Macrophages through Arachidonic Acid Signaling Pathway: An in vitro Study

Effects of Total Saponins from Dioscorea Nipponica Makino on Monosodium Urate-Induced M1-Polarized Macrophages through Arachidonic Acid Signaling Pathway: An in vitro Study

DOI：10.1007/s11655-022-3721-6

摘要

Abstract

关键词

Keywords

references